A Tensioned Human Skin Explant Model Used for Preliminary Assessment of Chemexfoliant-Stimulated Bioeffects
A tensioned ex vivo full-thickness human skin explant platform was used to assess the bioeffects arising from application of several commercial chemexfoliation agents. Although such treatments are well-established, and improved understanding of the underlying mechanistic processes continues to emerg...
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Elsevier
2025-01-01
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| Series: | JID Innovations |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667026724000523 |
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| author | Michael J. Conneely Jin Namkoong Francis Allison S. Kyoko Hirata Tsutsumi Dominic Grussu Ryan Willis Kyle Henderson Paul A. Campbell Melissa Moy Ewelina Lesniak Joanna Wu Robyn P. Hickerson |
| author_facet | Michael J. Conneely Jin Namkoong Francis Allison S. Kyoko Hirata Tsutsumi Dominic Grussu Ryan Willis Kyle Henderson Paul A. Campbell Melissa Moy Ewelina Lesniak Joanna Wu Robyn P. Hickerson |
| author_sort | Michael J. Conneely |
| collection | DOAJ |
| description | A tensioned ex vivo full-thickness human skin explant platform was used to assess the bioeffects arising from application of several commercial chemexfoliation agents. Although such treatments are well-established, and improved understanding of the underlying mechanistic processes continues to emerge, research into the optimum treatments for specific skin types/conditions is still needed for enhanced efficacy while minimizing recovery time. The 3 commercial chemexfoliation agents employed all contained trichloroacetic acid at well-defined concentrations (6, 10, and 20%) and were applied to the explants’ stratum corneum. Subsequently, measurements of dermal remodeling factors (COL1A1, ELN, HAS2, HAS3, and procollagen type I) and inflammatory marker (IL-1b) were undertaken using qPCR and immunofluorescent analyses. Statistical analysis of these data facilitated the establishment of benchmarking biological responses to these trichloroacetic acid–containing agents against untreated controls. The performance of an innovative trichloroacetic acid–free chemexfoliation agent was then measured and, upon comparison with the previous benchmarking data, indicated that dermal remodeling factors could be upregulated in fashion comparable with that of the trichloroacetic acid–containing agents but with significant suppression of inflammatory response. Our measurements thus underscore the promise of the tensioned explant over prolonged study periods and also that potentially valuable insights to guide preclinical strategies may be forthcoming from the protocol developed. |
| format | Article |
| id | doaj-art-10602dd15b5d4b14b936a972b7a54786 |
| institution | Kabale University |
| issn | 2667-0267 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JID Innovations |
| spelling | doaj-art-10602dd15b5d4b14b936a972b7a547862025-01-11T06:42:08ZengElsevierJID Innovations2667-02672025-01-0151100305A Tensioned Human Skin Explant Model Used for Preliminary Assessment of Chemexfoliant-Stimulated BioeffectsMichael J. Conneely0Jin Namkoong1Francis Allison2S. Kyoko Hirata Tsutsumi3Dominic Grussu4Ryan Willis5Kyle Henderson6Paul A. Campbell7Melissa Moy8Ewelina Lesniak9Joanna Wu10Robyn P. Hickerson11Ten Bio, Dundee, Scotland; Correspondence: Michael J. Conneely, Ten Bio, 30-34 Reform Street, Dundee DD1 RJ, Scotland.Research and Innovation, Department of Global Personal Care and Skin Health R&D, Colgate-Palmolive, Piscataway, New Jersey, USABiological Chemistry and Drug Discovery, School of Life Sciences, University of Dundee, Dundee, ScotlandTen Bio, Dundee, ScotlandTen Bio, Dundee, ScotlandTen Bio, Dundee, ScotlandTen Bio, Dundee, ScotlandTen Bio, Dundee, Scotland; PAUL, Carnegie Physics Laboratory, University of Dundee, Dundee, ScotlandPersonal Care Product Development, Skin Health R&D, Colgate-Palmolive, Piscataway, New Jersey, USAPersonal Care Product Development, Skin Health R&D, Colgate-Palmolive, Piscataway, New Jersey, USAResearch and Innovation, Department of Global Personal Care and Skin Health R&D, Colgate-Palmolive, Piscataway, New Jersey, USATen Bio, Dundee, Scotland; Biological Chemistry and Drug Discovery, School of Life Sciences, University of Dundee, Dundee, ScotlandA tensioned ex vivo full-thickness human skin explant platform was used to assess the bioeffects arising from application of several commercial chemexfoliation agents. Although such treatments are well-established, and improved understanding of the underlying mechanistic processes continues to emerge, research into the optimum treatments for specific skin types/conditions is still needed for enhanced efficacy while minimizing recovery time. The 3 commercial chemexfoliation agents employed all contained trichloroacetic acid at well-defined concentrations (6, 10, and 20%) and were applied to the explants’ stratum corneum. Subsequently, measurements of dermal remodeling factors (COL1A1, ELN, HAS2, HAS3, and procollagen type I) and inflammatory marker (IL-1b) were undertaken using qPCR and immunofluorescent analyses. Statistical analysis of these data facilitated the establishment of benchmarking biological responses to these trichloroacetic acid–containing agents against untreated controls. The performance of an innovative trichloroacetic acid–free chemexfoliation agent was then measured and, upon comparison with the previous benchmarking data, indicated that dermal remodeling factors could be upregulated in fashion comparable with that of the trichloroacetic acid–containing agents but with significant suppression of inflammatory response. Our measurements thus underscore the promise of the tensioned explant over prolonged study periods and also that potentially valuable insights to guide preclinical strategies may be forthcoming from the protocol developed.http://www.sciencedirect.com/science/article/pii/S2667026724000523ChemexfoliationChemical peelingEx vivo skin explantSkin rejuvenationTension |
| spellingShingle | Michael J. Conneely Jin Namkoong Francis Allison S. Kyoko Hirata Tsutsumi Dominic Grussu Ryan Willis Kyle Henderson Paul A. Campbell Melissa Moy Ewelina Lesniak Joanna Wu Robyn P. Hickerson A Tensioned Human Skin Explant Model Used for Preliminary Assessment of Chemexfoliant-Stimulated Bioeffects JID Innovations Chemexfoliation Chemical peeling Ex vivo skin explant Skin rejuvenation Tension |
| title | A Tensioned Human Skin Explant Model Used for Preliminary Assessment of Chemexfoliant-Stimulated Bioeffects |
| title_full | A Tensioned Human Skin Explant Model Used for Preliminary Assessment of Chemexfoliant-Stimulated Bioeffects |
| title_fullStr | A Tensioned Human Skin Explant Model Used for Preliminary Assessment of Chemexfoliant-Stimulated Bioeffects |
| title_full_unstemmed | A Tensioned Human Skin Explant Model Used for Preliminary Assessment of Chemexfoliant-Stimulated Bioeffects |
| title_short | A Tensioned Human Skin Explant Model Used for Preliminary Assessment of Chemexfoliant-Stimulated Bioeffects |
| title_sort | tensioned human skin explant model used for preliminary assessment of chemexfoliant stimulated bioeffects |
| topic | Chemexfoliation Chemical peeling Ex vivo skin explant Skin rejuvenation Tension |
| url | http://www.sciencedirect.com/science/article/pii/S2667026724000523 |
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