Photopharmacological modulation of hippocampal local field potential by caged‐glutamate with MicroLED probe
Abstract Aim Photopharmacology is a new technique for modulating biological phenomena through the photoconversion of substances in a specific target region at precise times. Caged compounds are thought to be compatible with photopharmacology as uncaged ligands are released and function in a light ir...
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| Format: | Article |
| Language: | English |
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Wiley
2024-09-01
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| Series: | Neuropsychopharmacology Reports |
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| Online Access: | https://doi.org/10.1002/npr2.12472 |
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| author | Shogo Okada Noriaki Ohkawa Kazuki Moriya Yoshito Saitoh Mikiko Ishikawa Kakeru Oya Atsushi Nishikawa Hiroto Sekiguchi |
| author_facet | Shogo Okada Noriaki Ohkawa Kazuki Moriya Yoshito Saitoh Mikiko Ishikawa Kakeru Oya Atsushi Nishikawa Hiroto Sekiguchi |
| author_sort | Shogo Okada |
| collection | DOAJ |
| description | Abstract Aim Photopharmacology is a new technique for modulating biological phenomena through the photoconversion of substances in a specific target region at precise times. Caged compounds are thought to be compatible with photopharmacology as uncaged ligands are released and function in a light irradiation‐dependent manner. Here, we investigated whether a microscale light‐emitting diode (MicroLED) probe is applicable for the photoconversion of caged‐glutamate (caged‐Glu) in vivo. Methods A needle‐shaped MicroLED probe was fabricated and inserted into the mouse hippocampal dentate gyrus (DG) with a cannula for drug injection and a recording electrode for measuring the local field potential (LFP). Artificial cerebrospinal fluid (ACSF) or caged‐Glu was infused into the DG and illuminated with light from a MicroLED probe. Results In the caged‐Glu‐injected DG, the LFP changed in the 10–20 Hz frequency ranges after light illumination, whereas there was no change in the ACSF control condition. Conclusion The MicroLED probe is applicable for photopharmacological experiments to modulate LFP with caged‐Glu in vivo. |
| format | Article |
| id | doaj-art-105c94baef6b4708bda2a9be49a59714 |
| institution | Kabale University |
| issn | 2574-173X |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neuropsychopharmacology Reports |
| spelling | doaj-art-105c94baef6b4708bda2a9be49a597142024-11-08T11:46:40ZengWileyNeuropsychopharmacology Reports2574-173X2024-09-0144365866210.1002/npr2.12472Photopharmacological modulation of hippocampal local field potential by caged‐glutamate with MicroLED probeShogo Okada0Noriaki Ohkawa1Kazuki Moriya2Yoshito Saitoh3Mikiko Ishikawa4Kakeru Oya5Atsushi Nishikawa6Hiroto Sekiguchi7Department of Electrical and Electronic Information Engineering Toyohashi University of Technology Toyohashi Aichi JapanDivision for Memory and Cognitive Function, Research Center for Advanced Medical Science, Comprehensive Research Facilities for Advanced Medical Science Dokkyo Medical University Tochigi JapanDepartment of Electrical and Electronic Information Engineering Toyohashi University of Technology Toyohashi Aichi JapanDivision for Memory and Cognitive Function, Research Center for Advanced Medical Science, Comprehensive Research Facilities for Advanced Medical Science Dokkyo Medical University Tochigi JapanDivision for Memory and Cognitive Function, Research Center for Advanced Medical Science, Comprehensive Research Facilities for Advanced Medical Science Dokkyo Medical University Tochigi JapanDepartment of Electrical and Electronic Information Engineering Toyohashi University of Technology Toyohashi Aichi JapanALLOS Semiconductors GmbH Dresden Sachsen GermanyDepartment of Electrical and Electronic Information Engineering Toyohashi University of Technology Toyohashi Aichi JapanAbstract Aim Photopharmacology is a new technique for modulating biological phenomena through the photoconversion of substances in a specific target region at precise times. Caged compounds are thought to be compatible with photopharmacology as uncaged ligands are released and function in a light irradiation‐dependent manner. Here, we investigated whether a microscale light‐emitting diode (MicroLED) probe is applicable for the photoconversion of caged‐glutamate (caged‐Glu) in vivo. Methods A needle‐shaped MicroLED probe was fabricated and inserted into the mouse hippocampal dentate gyrus (DG) with a cannula for drug injection and a recording electrode for measuring the local field potential (LFP). Artificial cerebrospinal fluid (ACSF) or caged‐Glu was infused into the DG and illuminated with light from a MicroLED probe. Results In the caged‐Glu‐injected DG, the LFP changed in the 10–20 Hz frequency ranges after light illumination, whereas there was no change in the ACSF control condition. Conclusion The MicroLED probe is applicable for photopharmacological experiments to modulate LFP with caged‐Glu in vivo.https://doi.org/10.1002/npr2.12472caged‐glutamatehippocampuslocal field potential (LFP)microscale light‐emitting diode (MicroLED)photopharmacology |
| spellingShingle | Shogo Okada Noriaki Ohkawa Kazuki Moriya Yoshito Saitoh Mikiko Ishikawa Kakeru Oya Atsushi Nishikawa Hiroto Sekiguchi Photopharmacological modulation of hippocampal local field potential by caged‐glutamate with MicroLED probe Neuropsychopharmacology Reports caged‐glutamate hippocampus local field potential (LFP) microscale light‐emitting diode (MicroLED) photopharmacology |
| title | Photopharmacological modulation of hippocampal local field potential by caged‐glutamate with MicroLED probe |
| title_full | Photopharmacological modulation of hippocampal local field potential by caged‐glutamate with MicroLED probe |
| title_fullStr | Photopharmacological modulation of hippocampal local field potential by caged‐glutamate with MicroLED probe |
| title_full_unstemmed | Photopharmacological modulation of hippocampal local field potential by caged‐glutamate with MicroLED probe |
| title_short | Photopharmacological modulation of hippocampal local field potential by caged‐glutamate with MicroLED probe |
| title_sort | photopharmacological modulation of hippocampal local field potential by caged glutamate with microled probe |
| topic | caged‐glutamate hippocampus local field potential (LFP) microscale light‐emitting diode (MicroLED) photopharmacology |
| url | https://doi.org/10.1002/npr2.12472 |
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