Galectin 9 rescues the inducibility of IL-10 expression in regulatory B cells of patients with food allergy
Abstract The deregulation of immune responses is what causes food allergy (FA) to occur. FA’s cause is still unknown. The goal of this study is to investigate the mechanism how the impaired production of IL-10 occurs in peripheral naive B cells of patients with FA. Samples from patients with FA and...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-84079-8 |
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| author | Huang Huang Minyao Li Shuo Song Shiyu Feng Xiaoyang Feng Yu Liu Pingchang Yang Pengyuan Zheng |
| author_facet | Huang Huang Minyao Li Shuo Song Shiyu Feng Xiaoyang Feng Yu Liu Pingchang Yang Pengyuan Zheng |
| author_sort | Huang Huang |
| collection | DOAJ |
| description | Abstract The deregulation of immune responses is what causes food allergy (FA) to occur. FA’s cause is still unknown. The goal of this study is to investigate the mechanism how the impaired production of IL-10 occurs in peripheral naive B cells of patients with FA. Samples from patients with FA and healthy controls (HC) were used to isolate CD19+ CD45R+ naive B cells from peripheral blood mononuclear cells (PBMC). Lipopolysaccharide (LPS) exposure was used to assess the expression of interleukin-10 (IL-10) in B cells. Although the FA and HC groups had similar total B cell counts, the FA patients had fewer IL-10+ B cell counts than the HC group. In peripheral B cells, the concentrations of IL-10 were inversely related to the concentrations of specific IgE, Th2 and Th1 cytokines in the serum. In patients with FA, peripheral B cells experienced impaired immune-suppressive functions. Galectin-9 could restore the defective induction of IL-10 expression in naive B cells of FA patients. In conclusion, FA patients with naive B cells experience impaired IL-10 induction. The induction of IL-10 in naive B cells of FA patients can be restored by galectin-9 treatment, which triggers B cells to differentiate into immune regulatory B cells. |
| format | Article |
| id | doaj-art-10354f20b39c474b8fccdd2517f62931 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-10354f20b39c474b8fccdd2517f629312025-01-05T12:14:36ZengNature PortfolioScientific Reports2045-23222025-01-0115111110.1038/s41598-024-84079-8Galectin 9 rescues the inducibility of IL-10 expression in regulatory B cells of patients with food allergyHuang Huang0Minyao Li1Shuo Song2Shiyu Feng3Xiaoyang Feng4Yu Liu5Pingchang Yang6Pengyuan Zheng7Department of Gastroenterology, Fifth Affiliated Hospital, Zhengzhou UniversityDepartment of General Practice Medicine, Third Affiliated Hospital of Shenzhen UniversityDepartment of General Practice Medicine, Third Affiliated Hospital of Shenzhen UniversityDepartment of Traditional Chinese Medicine, Affiliated Shenzhen Maternity & Child Healthcare Hospital, Southern Medical UniversityDepartment of Traditional Chinese Medicine, Affiliated Shenzhen Maternity & Child Healthcare Hospital, Southern Medical UniversityDepartment of General Practice Medicine, Third Affiliated Hospital of Shenzhen UniversityInstitute of Allergy & Immunology of Shenzhen University, State Key Laboratory of Respiratory Diseases Allergy Division, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen UniversityDepartment of Gastroenterology, Fifth Affiliated Hospital, Zhengzhou UniversityAbstract The deregulation of immune responses is what causes food allergy (FA) to occur. FA’s cause is still unknown. The goal of this study is to investigate the mechanism how the impaired production of IL-10 occurs in peripheral naive B cells of patients with FA. Samples from patients with FA and healthy controls (HC) were used to isolate CD19+ CD45R+ naive B cells from peripheral blood mononuclear cells (PBMC). Lipopolysaccharide (LPS) exposure was used to assess the expression of interleukin-10 (IL-10) in B cells. Although the FA and HC groups had similar total B cell counts, the FA patients had fewer IL-10+ B cell counts than the HC group. In peripheral B cells, the concentrations of IL-10 were inversely related to the concentrations of specific IgE, Th2 and Th1 cytokines in the serum. In patients with FA, peripheral B cells experienced impaired immune-suppressive functions. Galectin-9 could restore the defective induction of IL-10 expression in naive B cells of FA patients. In conclusion, FA patients with naive B cells experience impaired IL-10 induction. The induction of IL-10 in naive B cells of FA patients can be restored by galectin-9 treatment, which triggers B cells to differentiate into immune regulatory B cells.https://doi.org/10.1038/s41598-024-84079-8Food allergyB cellIL-10ImmunityImmune regulation |
| spellingShingle | Huang Huang Minyao Li Shuo Song Shiyu Feng Xiaoyang Feng Yu Liu Pingchang Yang Pengyuan Zheng Galectin 9 rescues the inducibility of IL-10 expression in regulatory B cells of patients with food allergy Scientific Reports Food allergy B cell IL-10 Immunity Immune regulation |
| title | Galectin 9 rescues the inducibility of IL-10 expression in regulatory B cells of patients with food allergy |
| title_full | Galectin 9 rescues the inducibility of IL-10 expression in regulatory B cells of patients with food allergy |
| title_fullStr | Galectin 9 rescues the inducibility of IL-10 expression in regulatory B cells of patients with food allergy |
| title_full_unstemmed | Galectin 9 rescues the inducibility of IL-10 expression in regulatory B cells of patients with food allergy |
| title_short | Galectin 9 rescues the inducibility of IL-10 expression in regulatory B cells of patients with food allergy |
| title_sort | galectin 9 rescues the inducibility of il 10 expression in regulatory b cells of patients with food allergy |
| topic | Food allergy B cell IL-10 Immunity Immune regulation |
| url | https://doi.org/10.1038/s41598-024-84079-8 |
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