Isolation and genetic characterization of Toxoplasma gondii from chickens from public markets in Pernambuco State, Brazil
This study aimed to evaluate the presence and viability of Toxoplasma gondii in chickens intended for human consumption in the Pernambuco State, Brazil. Blood and tissue samples were collected from 25 chickens sold in markets in Recife, Pernambuco. Samples were evaluated by indirect immunofluorescen...
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Elsevier
2024-01-01
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| Series: | Current Research in Parasitology and Vector-Borne Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667114X24000384 |
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| author | Renato Amorim da Silva Raissa Santana Renovato Hannah Tsuruzaki Kirzner de Barros e Silva Maria Luiza Didier Marques Pollyanne Raysa Fernandes Oliveira Jéssica de Crasto Souza Carvalho-Reis Paul M. Bartley Frank Katzer Érika Fernanda Torres Samico-Fernandes Renata Pimentel Bandeira de Melo Rinaldo Aparecido Mota |
| author_facet | Renato Amorim da Silva Raissa Santana Renovato Hannah Tsuruzaki Kirzner de Barros e Silva Maria Luiza Didier Marques Pollyanne Raysa Fernandes Oliveira Jéssica de Crasto Souza Carvalho-Reis Paul M. Bartley Frank Katzer Érika Fernanda Torres Samico-Fernandes Renata Pimentel Bandeira de Melo Rinaldo Aparecido Mota |
| author_sort | Renato Amorim da Silva |
| collection | DOAJ |
| description | This study aimed to evaluate the presence and viability of Toxoplasma gondii in chickens intended for human consumption in the Pernambuco State, Brazil. Blood and tissue samples were collected from 25 chickens sold in markets in Recife, Pernambuco. Samples were evaluated by indirect immunofluorescence assay (IFA) to detect antibodies to T. gondii. Pools of brain and heart of seropositive chickens were subjected to bioassay in two Swiss Webster mice, which were evaluated for 45 days then tested by IFA to detect seroconversion. The mice were euthanized, and their brains were evaluated for cysts. Peritoneal lavage was also conducted in mice that exhibited clinical signs. Brains containing cysts or peritoneal lavage with tachyzoites were inoculated into MA-104 cells. Brains of mice inoculated with the same tissue were pooled and analysed by ITS1-PCR. We obtained a frequency of antibodies to T. gondii of 68.00% (17/25) in chickens, and a seroconversion rate of 70.58% (24/34) in mice. Detection of Toxoplasma ITS1 DNA confirmed an isolation rate of 41.1% (7/17). Three isolates were characterized by mnPCR-RFLP as genotypes ToxoDB#36 and ToxoDB#114. We highlight the occurrence of ToxoDB#36 in chickens in Pernambuco State and the parasitesʼ viability in chickens intended for human consumption. |
| format | Article |
| id | doaj-art-10293ee3bae844e891a0a03b89c73bc0 |
| institution | Kabale University |
| issn | 2667-114X |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
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| series | Current Research in Parasitology and Vector-Borne Diseases |
| spelling | doaj-art-10293ee3bae844e891a0a03b89c73bc02024-11-30T07:14:29ZengElsevierCurrent Research in Parasitology and Vector-Borne Diseases2667-114X2024-01-016100207Isolation and genetic characterization of Toxoplasma gondii from chickens from public markets in Pernambuco State, BrazilRenato Amorim da Silva0Raissa Santana Renovato1Hannah Tsuruzaki Kirzner de Barros e Silva2Maria Luiza Didier Marques3Pollyanne Raysa Fernandes Oliveira4Jéssica de Crasto Souza Carvalho-Reis5Paul M. Bartley6Frank Katzer7Érika Fernanda Torres Samico-Fernandes8Renata Pimentel Bandeira de Melo9Rinaldo Aparecido Mota10Department of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, ZC 52171-900, BrazilDepartment of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, ZC 52171-900, BrazilDepartment of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, ZC 52171-900, BrazilDepartment of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, ZC 52171-900, BrazilDepartment of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, ZC 52171-900, BrazilDepartment of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, ZC 52171-900, BrazilMoredun Research Institute, Pentlands Science Park, Penicuik, EH26 0PZ, Scotland, United KingdomMoredun Research Institute, Pentlands Science Park, Penicuik, EH26 0PZ, Scotland, United KingdomDepartment of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, ZC 52171-900, BrazilDepartment of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, ZC 52171-900, Brazil; Corresponding author.Department of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, ZC 52171-900, BrazilThis study aimed to evaluate the presence and viability of Toxoplasma gondii in chickens intended for human consumption in the Pernambuco State, Brazil. Blood and tissue samples were collected from 25 chickens sold in markets in Recife, Pernambuco. Samples were evaluated by indirect immunofluorescence assay (IFA) to detect antibodies to T. gondii. Pools of brain and heart of seropositive chickens were subjected to bioassay in two Swiss Webster mice, which were evaluated for 45 days then tested by IFA to detect seroconversion. The mice were euthanized, and their brains were evaluated for cysts. Peritoneal lavage was also conducted in mice that exhibited clinical signs. Brains containing cysts or peritoneal lavage with tachyzoites were inoculated into MA-104 cells. Brains of mice inoculated with the same tissue were pooled and analysed by ITS1-PCR. We obtained a frequency of antibodies to T. gondii of 68.00% (17/25) in chickens, and a seroconversion rate of 70.58% (24/34) in mice. Detection of Toxoplasma ITS1 DNA confirmed an isolation rate of 41.1% (7/17). Three isolates were characterized by mnPCR-RFLP as genotypes ToxoDB#36 and ToxoDB#114. We highlight the occurrence of ToxoDB#36 in chickens in Pernambuco State and the parasitesʼ viability in chickens intended for human consumption.http://www.sciencedirect.com/science/article/pii/S2667114X24000384Toxoplasma gondiiChickenMouse bioassayPCR-RFLPPublic healthZoonosis |
| spellingShingle | Renato Amorim da Silva Raissa Santana Renovato Hannah Tsuruzaki Kirzner de Barros e Silva Maria Luiza Didier Marques Pollyanne Raysa Fernandes Oliveira Jéssica de Crasto Souza Carvalho-Reis Paul M. Bartley Frank Katzer Érika Fernanda Torres Samico-Fernandes Renata Pimentel Bandeira de Melo Rinaldo Aparecido Mota Isolation and genetic characterization of Toxoplasma gondii from chickens from public markets in Pernambuco State, Brazil Current Research in Parasitology and Vector-Borne Diseases Toxoplasma gondii Chicken Mouse bioassay PCR-RFLP Public health Zoonosis |
| title | Isolation and genetic characterization of Toxoplasma gondii from chickens from public markets in Pernambuco State, Brazil |
| title_full | Isolation and genetic characterization of Toxoplasma gondii from chickens from public markets in Pernambuco State, Brazil |
| title_fullStr | Isolation and genetic characterization of Toxoplasma gondii from chickens from public markets in Pernambuco State, Brazil |
| title_full_unstemmed | Isolation and genetic characterization of Toxoplasma gondii from chickens from public markets in Pernambuco State, Brazil |
| title_short | Isolation and genetic characterization of Toxoplasma gondii from chickens from public markets in Pernambuco State, Brazil |
| title_sort | isolation and genetic characterization of toxoplasma gondii from chickens from public markets in pernambuco state brazil |
| topic | Toxoplasma gondii Chicken Mouse bioassay PCR-RFLP Public health Zoonosis |
| url | http://www.sciencedirect.com/science/article/pii/S2667114X24000384 |
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