Influence of nutrient medium and time on lipopeptide synthesis in Bacillus subtilis GM5
The level of expression of srfAD gene, a subunit AD of surfactin synthetase, and the yield of the total lipopeptide fraction were studied in relation to the composition of nutrient medium and the growth stage of the probiotic strain Bacillus subtilis GM5. The maximum concentration of the total lipo...
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| Language: | English |
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EDP Sciences
2024-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2024/68/bioconf_geneticresources2024_01062.pdf |
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| author | Lutfullina Guzel Abubakirova Adela Lutfullin Marat Mardanova Ayslu |
| author_facet | Lutfullina Guzel Abubakirova Adela Lutfullin Marat Mardanova Ayslu |
| author_sort | Lutfullina Guzel |
| collection | DOAJ |
| description | The level of expression of
srfAD gene, a subunit AD of surfactin synthetase, and the yield of the total lipopeptide fraction were studied in relation to the composition of nutrient medium and the growth stage of the probiotic strain Bacillus subtilis GM5. The maximum concentration of the total lipopeptide fraction was observed during the growth of strain GM5 on SMN medium with soybean flour. The relative expression of the srfAD gene of B. subtilis GM5 was quantified by RT- qPCR. We conclude that the increase in the concentration of the total lipopeptide fraction on SMN medium correlates with the expression of the srfAD gene during 96-hour cultivation of GM5, which may indicate the production of mainly lipopeptide from the surfactin group by under these conditions. |
| format | Article |
| id | doaj-art-100dbd32a2214d4d8098a0f542f4de9c |
| institution | Kabale University |
| issn | 2117-4458 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-100dbd32a2214d4d8098a0f542f4de9c2024-12-06T09:34:14ZengEDP SciencesBIO Web of Conferences2117-44582024-01-011490106210.1051/bioconf/202414901062bioconf_geneticresources2024_01062Influence of nutrient medium and time on lipopeptide synthesis in Bacillus subtilis GM5Lutfullina Guzel0Abubakirova Adela1Lutfullin Marat2Mardanova Ayslu3Kazan (Volga Region), Institute of Fundamental Medicine and Biology, Federal UniversityKazan (Volga Region), Institute of Fundamental Medicine and Biology, Federal UniversityKazan (Volga Region), Institute of Fundamental Medicine and Biology, Federal UniversityKazan (Volga Region), Institute of Fundamental Medicine and Biology, Federal UniversityThe level of expression of srfAD gene, a subunit AD of surfactin synthetase, and the yield of the total lipopeptide fraction were studied in relation to the composition of nutrient medium and the growth stage of the probiotic strain Bacillus subtilis GM5. The maximum concentration of the total lipopeptide fraction was observed during the growth of strain GM5 on SMN medium with soybean flour. The relative expression of the srfAD gene of B. subtilis GM5 was quantified by RT- qPCR. We conclude that the increase in the concentration of the total lipopeptide fraction on SMN medium correlates with the expression of the srfAD gene during 96-hour cultivation of GM5, which may indicate the production of mainly lipopeptide from the surfactin group by under these conditions.https://www.bio-conferences.org/articles/bioconf/pdf/2024/68/bioconf_geneticresources2024_01062.pdf |
| spellingShingle | Lutfullina Guzel Abubakirova Adela Lutfullin Marat Mardanova Ayslu Influence of nutrient medium and time on lipopeptide synthesis in Bacillus subtilis GM5 BIO Web of Conferences |
| title | Influence of nutrient medium and time on lipopeptide synthesis in Bacillus subtilis GM5 |
| title_full | Influence of nutrient medium and time on lipopeptide synthesis in Bacillus subtilis GM5 |
| title_fullStr | Influence of nutrient medium and time on lipopeptide synthesis in Bacillus subtilis GM5 |
| title_full_unstemmed | Influence of nutrient medium and time on lipopeptide synthesis in Bacillus subtilis GM5 |
| title_short | Influence of nutrient medium and time on lipopeptide synthesis in Bacillus subtilis GM5 |
| title_sort | influence of nutrient medium and time on lipopeptide synthesis in bacillus subtilis gm5 |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2024/68/bioconf_geneticresources2024_01062.pdf |
| work_keys_str_mv | AT lutfullinaguzel influenceofnutrientmediumandtimeonlipopeptidesynthesisinbacillussubtilisgm5 AT abubakirovaadela influenceofnutrientmediumandtimeonlipopeptidesynthesisinbacillussubtilisgm5 AT lutfullinmarat influenceofnutrientmediumandtimeonlipopeptidesynthesisinbacillussubtilisgm5 AT mardanovaayslu influenceofnutrientmediumandtimeonlipopeptidesynthesisinbacillussubtilisgm5 |