Whole genome sequencing, assembly and annotation of the Southern Ground Hornbill – Bucorvus leadbeateri

Abstract The Southern Ground Hornbill (SGH – Bucorvus leadbeateri) is one of the largest hornbill species worldwide, known for its complex social structures and breeding behaviours. This bird has been of great interest due to its declining population and disappearance from historic ranges in souther...

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Main Authors: Jasmin Patel, Angela Botes, Jean Mollett, Pieter De Maayer
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-04412-2
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author Jasmin Patel
Angela Botes
Jean Mollett
Pieter De Maayer
author_facet Jasmin Patel
Angela Botes
Jean Mollett
Pieter De Maayer
author_sort Jasmin Patel
collection DOAJ
description Abstract The Southern Ground Hornbill (SGH – Bucorvus leadbeateri) is one of the largest hornbill species worldwide, known for its complex social structures and breeding behaviours. This bird has been of great interest due to its declining population and disappearance from historic ranges in southern Africa. Despite being the focus of numerous conservation efforts, with research forming an integral part of these initiatives, there is still a substantial lack of knowledge regarding the molecular biology aspects of this bird species. In this study, whole genome sequencing of the SGH was achieved using Illumina short-read (NovaSeq 6000) and Pacific Biosciences long-read technologies. A hybrid de novo genome assembly followed by reference-based refinement produced a 1.16 Gb high-quality draft genome assembly of the SGH comprised of 1,672 contigs (N50 value of 40.45 Mb). The availability of this genome will aid in improving our current understanding of this bird at a genomic level and provide a pivotal foundation for comparative genomic analysis with other hornbill species.
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spelling doaj-art-0fb2cdbd92d649fcafa43f06fe330bfa2025-01-12T12:07:36ZengNature PortfolioScientific Data2052-44632025-01-011211710.1038/s41597-025-04412-2Whole genome sequencing, assembly and annotation of the Southern Ground Hornbill – Bucorvus leadbeateriJasmin Patel0Angela Botes1Jean Mollett2Pieter De Maayer3School of Molecular and Cell Biology, University of the WitwatersrandSchool of Molecular and Cell Biology, University of the WitwatersrandSchool of Molecular and Cell Biology, University of the WitwatersrandSchool of Molecular and Cell Biology, University of the WitwatersrandAbstract The Southern Ground Hornbill (SGH – Bucorvus leadbeateri) is one of the largest hornbill species worldwide, known for its complex social structures and breeding behaviours. This bird has been of great interest due to its declining population and disappearance from historic ranges in southern Africa. Despite being the focus of numerous conservation efforts, with research forming an integral part of these initiatives, there is still a substantial lack of knowledge regarding the molecular biology aspects of this bird species. In this study, whole genome sequencing of the SGH was achieved using Illumina short-read (NovaSeq 6000) and Pacific Biosciences long-read technologies. A hybrid de novo genome assembly followed by reference-based refinement produced a 1.16 Gb high-quality draft genome assembly of the SGH comprised of 1,672 contigs (N50 value of 40.45 Mb). The availability of this genome will aid in improving our current understanding of this bird at a genomic level and provide a pivotal foundation for comparative genomic analysis with other hornbill species.https://doi.org/10.1038/s41597-025-04412-2
spellingShingle Jasmin Patel
Angela Botes
Jean Mollett
Pieter De Maayer
Whole genome sequencing, assembly and annotation of the Southern Ground Hornbill – Bucorvus leadbeateri
Scientific Data
title Whole genome sequencing, assembly and annotation of the Southern Ground Hornbill – Bucorvus leadbeateri
title_full Whole genome sequencing, assembly and annotation of the Southern Ground Hornbill – Bucorvus leadbeateri
title_fullStr Whole genome sequencing, assembly and annotation of the Southern Ground Hornbill – Bucorvus leadbeateri
title_full_unstemmed Whole genome sequencing, assembly and annotation of the Southern Ground Hornbill – Bucorvus leadbeateri
title_short Whole genome sequencing, assembly and annotation of the Southern Ground Hornbill – Bucorvus leadbeateri
title_sort whole genome sequencing assembly and annotation of the southern ground hornbill bucorvus leadbeateri
url https://doi.org/10.1038/s41597-025-04412-2
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