A novel lncRNA-hidden polypeptide regulates malignant phenotypes and pemetrexed sensitivity in A549 pulmonary adenocarcinoma cells
Abstract The advance of high-throughput sequencing enhances the discovery of short ORFs embedded in long non-coding RNAs (lncRNAs). Here, we uncovered the production and biological activity of lncRNA-hidden polypeptides in lung adenocarcinoma (LUAD). In the present study, bioinformatics was used to...
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| Format: | Article |
| Language: | English |
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Springer
2024-02-01
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| Series: | Amino Acids |
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| Online Access: | https://doi.org/10.1007/s00726-023-03361-7 |
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| author | Xiaobing Han Liangxin Chen Peng Sun Xiuqing Wang Qian Zhao Lingfeng Liao Dejin Lou Nan Zhou Yujun Wang |
| author_facet | Xiaobing Han Liangxin Chen Peng Sun Xiuqing Wang Qian Zhao Lingfeng Liao Dejin Lou Nan Zhou Yujun Wang |
| author_sort | Xiaobing Han |
| collection | DOAJ |
| description | Abstract The advance of high-throughput sequencing enhances the discovery of short ORFs embedded in long non-coding RNAs (lncRNAs). Here, we uncovered the production and biological activity of lncRNA-hidden polypeptides in lung adenocarcinoma (LUAD). In the present study, bioinformatics was used to screen the lncRNA-hidden polypeptides in LUAD. Analysis of protein expression was done by western blot or immunofluorescence assay. The functions of the polypeptide were determined by detecting its effects on cell viability, proliferation, migration, invasion, and pemetrexed (PEM) sensitivity. The protein interactors of the polypeptide were analyzed by mass spectrometry after Co-immunoprecipitation (Co-IP) assay. The results showed that the lncRNA LINC00954 was confirmed to encode a novel polypeptide LINC00954-ORF. The polypeptide had tumor-suppressor features in A549 cells by repressing cell growth, motility and invasion. Moreover, the polypeptide enhanced PEM sensitivity and suppressed growth in A549/PEM cells. The protein interactors of this polypeptide had close correlations with RNA processing, amide metabolic process, translation, RNA binding, RNA transport, and DNA replication. As a conclusion, the LINC00954-ORF polypeptide embedded in lncRNA LINC00954 possesses tumor-suppressor features in A549 and PEM-resistant A549 cells and sensitizes PEM-resistant A549 cells to PEM, providing evidence that the LINC00954-ORF polypeptide is a potential anti-cancer agent in LUAD. |
| format | Article |
| id | doaj-art-0faef48a5f6e4ceaa2ee19dd330bd52e |
| institution | Kabale University |
| issn | 1438-2199 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Springer |
| record_format | Article |
| series | Amino Acids |
| spelling | doaj-art-0faef48a5f6e4ceaa2ee19dd330bd52e2024-12-22T12:34:48ZengSpringerAmino Acids1438-21992024-02-0156111010.1007/s00726-023-03361-7A novel lncRNA-hidden polypeptide regulates malignant phenotypes and pemetrexed sensitivity in A549 pulmonary adenocarcinoma cellsXiaobing Han0Liangxin Chen1Peng Sun2Xiuqing Wang3Qian Zhao4Lingfeng Liao5Dejin Lou6Nan Zhou7Yujun Wang8Department of Oncology, Xinyang Central HospitalDepartment of Oncology, Xinyang Central HospitalDepartment of Oncology, Xinyang Central HospitalDepartment of Oncology, Xinyang Central HospitalDepartment of Oncology, Xinyang Central HospitalDepartment of Oncology, Xinyang Central HospitalDepartment of Oncology, Xinyang Central HospitalDepartment of Oncology, Xinyang Central HospitalDepartment of Gastroenterology, Xinyang Central HospitalAbstract The advance of high-throughput sequencing enhances the discovery of short ORFs embedded in long non-coding RNAs (lncRNAs). Here, we uncovered the production and biological activity of lncRNA-hidden polypeptides in lung adenocarcinoma (LUAD). In the present study, bioinformatics was used to screen the lncRNA-hidden polypeptides in LUAD. Analysis of protein expression was done by western blot or immunofluorescence assay. The functions of the polypeptide were determined by detecting its effects on cell viability, proliferation, migration, invasion, and pemetrexed (PEM) sensitivity. The protein interactors of the polypeptide were analyzed by mass spectrometry after Co-immunoprecipitation (Co-IP) assay. The results showed that the lncRNA LINC00954 was confirmed to encode a novel polypeptide LINC00954-ORF. The polypeptide had tumor-suppressor features in A549 cells by repressing cell growth, motility and invasion. Moreover, the polypeptide enhanced PEM sensitivity and suppressed growth in A549/PEM cells. The protein interactors of this polypeptide had close correlations with RNA processing, amide metabolic process, translation, RNA binding, RNA transport, and DNA replication. As a conclusion, the LINC00954-ORF polypeptide embedded in lncRNA LINC00954 possesses tumor-suppressor features in A549 and PEM-resistant A549 cells and sensitizes PEM-resistant A549 cells to PEM, providing evidence that the LINC00954-ORF polypeptide is a potential anti-cancer agent in LUAD.https://doi.org/10.1007/s00726-023-03361-7NSCLClncRNA-hidden polypeptidesShort ORFsLINC00954-ORFPEM resistance |
| spellingShingle | Xiaobing Han Liangxin Chen Peng Sun Xiuqing Wang Qian Zhao Lingfeng Liao Dejin Lou Nan Zhou Yujun Wang A novel lncRNA-hidden polypeptide regulates malignant phenotypes and pemetrexed sensitivity in A549 pulmonary adenocarcinoma cells Amino Acids NSCLC lncRNA-hidden polypeptides Short ORFs LINC00954-ORF PEM resistance |
| title | A novel lncRNA-hidden polypeptide regulates malignant phenotypes and pemetrexed sensitivity in A549 pulmonary adenocarcinoma cells |
| title_full | A novel lncRNA-hidden polypeptide regulates malignant phenotypes and pemetrexed sensitivity in A549 pulmonary adenocarcinoma cells |
| title_fullStr | A novel lncRNA-hidden polypeptide regulates malignant phenotypes and pemetrexed sensitivity in A549 pulmonary adenocarcinoma cells |
| title_full_unstemmed | A novel lncRNA-hidden polypeptide regulates malignant phenotypes and pemetrexed sensitivity in A549 pulmonary adenocarcinoma cells |
| title_short | A novel lncRNA-hidden polypeptide regulates malignant phenotypes and pemetrexed sensitivity in A549 pulmonary adenocarcinoma cells |
| title_sort | novel lncrna hidden polypeptide regulates malignant phenotypes and pemetrexed sensitivity in a549 pulmonary adenocarcinoma cells |
| topic | NSCLC lncRNA-hidden polypeptides Short ORFs LINC00954-ORF PEM resistance |
| url | https://doi.org/10.1007/s00726-023-03361-7 |
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