In-Vitro Effectiveness of Ethanol Extract of Permot Leaf (Passiflora foetida Linn.) Towards Mortality of Rhipicephalus sanguineus Larvae

This study had three fold objective which were finding the effectiveness of ethanol extract of permot leaf (Passiflora foetida Linn.) towards mortality of Rhpicephalus sanguineus (R. sanguineus) larvae in vitro and also identifying lethal concentration and lethal time. Completely randomized design w...

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Main Authors: Intan Nurcahya, Poedji Hastutiek, Ratna Damayanti, Iwan Sahrial Hamid, Agus Wijaya
Format: Article
Language:English
Published: Universitas Airlangga 2020-09-01
Series:Journal of Parasite Science
Subjects:
Online Access:https://e-journal.unair.ac.id/JoPS/article/view/29155
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author Intan Nurcahya
Poedji Hastutiek
Ratna Damayanti
Iwan Sahrial Hamid
Agus Wijaya
author_facet Intan Nurcahya
Poedji Hastutiek
Ratna Damayanti
Iwan Sahrial Hamid
Agus Wijaya
author_sort Intan Nurcahya
collection DOAJ
description This study had three fold objective which were finding the effectiveness of ethanol extract of permot leaf (Passiflora foetida Linn.) towards mortality of Rhpicephalus sanguineus (R. sanguineus) larvae in vitro and also identifying lethal concentration and lethal time. Completely randomized design was used with six treatments and four replications. Five R. sanguineus were used in the treatment for all replication. The observation of mortality was done every ten minutes in five hours. Rhpicephalus sanguineus larvae were dead if there was no movement. The collected data was analyzed using ANOVA factorial and followed by Duncan's test. The obtained data showed that ethanol extract of permot's leaf ethanol extract was effective toward mortality of R. sanguineus larvae in vitro. The suspension of 10% extract concentration of permot leaf ethanol extract has a similar effectiveness as Noticks®. Probit analysis stated that LC50 can be reached by using the 4% concentration in two hours and 2.4% concentration in five hours. LC95 can be reached by using the 6.5% concentration in two hours and 3.93% concentration in five hours. The quickest LT50 can be reached in 0.91 hours and LT95 ­2.37 hours after treatment with 10% concentration.
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id doaj-art-0fa6b91351e84d6fb6fb9b17db9de4f8
institution Kabale University
issn 2599-0993
2656-5331
language English
publishDate 2020-09-01
publisher Universitas Airlangga
record_format Article
series Journal of Parasite Science
spelling doaj-art-0fa6b91351e84d6fb6fb9b17db9de4f82025-01-09T04:38:14ZengUniversitas AirlanggaJournal of Parasite Science2599-09932656-53312020-09-0142616410.20473/jops.v4i2.2915523862In-Vitro Effectiveness of Ethanol Extract of Permot Leaf (Passiflora foetida Linn.) Towards Mortality of Rhipicephalus sanguineus LarvaeIntan NurcahyaPoedji HastutiekRatna DamayantiIwan Sahrial HamidAgus WijayaThis study had three fold objective which were finding the effectiveness of ethanol extract of permot leaf (Passiflora foetida Linn.) towards mortality of Rhpicephalus sanguineus (R. sanguineus) larvae in vitro and also identifying lethal concentration and lethal time. Completely randomized design was used with six treatments and four replications. Five R. sanguineus were used in the treatment for all replication. The observation of mortality was done every ten minutes in five hours. Rhpicephalus sanguineus larvae were dead if there was no movement. The collected data was analyzed using ANOVA factorial and followed by Duncan's test. The obtained data showed that ethanol extract of permot's leaf ethanol extract was effective toward mortality of R. sanguineus larvae in vitro. The suspension of 10% extract concentration of permot leaf ethanol extract has a similar effectiveness as Noticks®. Probit analysis stated that LC50 can be reached by using the 4% concentration in two hours and 2.4% concentration in five hours. LC95 can be reached by using the 6.5% concentration in two hours and 3.93% concentration in five hours. The quickest LT50 can be reached in 0.91 hours and LT95 ­2.37 hours after treatment with 10% concentration.https://e-journal.unair.ac.id/JoPS/article/view/29155ethanol extractpermot leafin vitrorhipicephalus sanguineuspassiflora foetida
spellingShingle Intan Nurcahya
Poedji Hastutiek
Ratna Damayanti
Iwan Sahrial Hamid
Agus Wijaya
In-Vitro Effectiveness of Ethanol Extract of Permot Leaf (Passiflora foetida Linn.) Towards Mortality of Rhipicephalus sanguineus Larvae
Journal of Parasite Science
ethanol extract
permot leaf
in vitro
rhipicephalus sanguineus
passiflora foetida
title In-Vitro Effectiveness of Ethanol Extract of Permot Leaf (Passiflora foetida Linn.) Towards Mortality of Rhipicephalus sanguineus Larvae
title_full In-Vitro Effectiveness of Ethanol Extract of Permot Leaf (Passiflora foetida Linn.) Towards Mortality of Rhipicephalus sanguineus Larvae
title_fullStr In-Vitro Effectiveness of Ethanol Extract of Permot Leaf (Passiflora foetida Linn.) Towards Mortality of Rhipicephalus sanguineus Larvae
title_full_unstemmed In-Vitro Effectiveness of Ethanol Extract of Permot Leaf (Passiflora foetida Linn.) Towards Mortality of Rhipicephalus sanguineus Larvae
title_short In-Vitro Effectiveness of Ethanol Extract of Permot Leaf (Passiflora foetida Linn.) Towards Mortality of Rhipicephalus sanguineus Larvae
title_sort in vitro effectiveness of ethanol extract of permot leaf passiflora foetida linn towards mortality of rhipicephalus sanguineus larvae
topic ethanol extract
permot leaf
in vitro
rhipicephalus sanguineus
passiflora foetida
url https://e-journal.unair.ac.id/JoPS/article/view/29155
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