A review of 3D bioprinting for organoids
Current two-dimensional (2D) cell models for effective drug screening suffer from significant limitations imposed by the lack of realism in the physiological environment. Three-dimensional (3D) organoids models hold immense potential in mimicking the key functions of human organs by overcoming the l...
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| Format: | Article |
| Language: | English |
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De Gruyter
2025-01-01
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| Series: | Medical Review |
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| Online Access: | https://doi.org/10.1515/mr-2024-0089 |
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| author | Li Zeqing Chen Long Wu Jialin Chen Yikang Zhu Yizhun Li Gang Xie Guoxi Tang Guosheng Xie Maobin |
| author_facet | Li Zeqing Chen Long Wu Jialin Chen Yikang Zhu Yizhun Li Gang Xie Guoxi Tang Guosheng Xie Maobin |
| author_sort | Li Zeqing |
| collection | DOAJ |
| description | Current two-dimensional (2D) cell models for effective drug screening suffer from significant limitations imposed by the lack of realism in the physiological environment. Three-dimensional (3D) organoids models hold immense potential in mimicking the key functions of human organs by overcoming the limitations of traditional 2D cell models. However, current techniques for preparation of 3D organoids models had limitations in reproducibility, scalability, and the ability to closely replicate the complex microenvironment found in vivo. Additionally, traditional 3D cell culture systems often involve lengthy and labor-intensive processes that hinder high-throughput applications necessary for a large-scale drug screening. Advancements in 3D bioprinting technologies offer promising solutions to these challenges by enabling precise spatial control over cell placement and material composition, thereby facilitating the creation of more physiologically relevant organoids than current techniques. This review provides a comprehensive summary of recent advances in 3D bioprinting technologies for creating organoids models, which begins with an introduction to different types of 3D bioprinting techniques (especially focus on volumetric bioprinting (VBP) technique), followed by an overview of bioinks utilized for organoids bioprinting. Moreover, we also introduce the applications of 3D bioprinting organoids in disease models, drug efficiency evaluation and regenerative medicine. Finally, the challenges and possible strategies for the development and clinical translation of 3D bioprinting organoids are concluded. |
| format | Article |
| id | doaj-art-0f6b75f6feba4e5b804b851a620b3c82 |
| institution | Kabale University |
| issn | 2749-9642 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Medical Review |
| spelling | doaj-art-0f6b75f6feba4e5b804b851a620b3c822025-08-25T06:10:55ZengDe GruyterMedical Review2749-96422025-01-015431833810.1515/mr-2024-0089A review of 3D bioprinting for organoidsLi Zeqing0Chen Long1Wu Jialin2Chen Yikang3Zhu Yizhun4Li Gang5Xie Guoxi6Tang Guosheng7Xie Maobin8The Fourth Affiliated Hospital of Guangzhou Medical University, School of Biomedical Engineering, Guangzhou Medical University, Guangzhou, Guangdong, ChinaThe Fourth Affiliated Hospital of Guangzhou Medical University, School of Biomedical Engineering, Guangzhou Medical University, Guangzhou, Guangdong, ChinaGuangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, The NMPA and State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital, Guangzhou Medical University26468, Guangzhou, Guangdong, ChinaSchool of Pharmacy & State Key Laboratory of Quality Research in Chinese Medicine and Laboratory of Drug Discovery from Natural Resources and Industrialization, Macau University of Science and Technology, Macau SAR, ChinaSchool of Pharmacy & State Key Laboratory of Quality Research in Chinese Medicine and Laboratory of Drug Discovery from Natural Resources and Industrialization, Macau University of Science and Technology, Macau SAR, ChinaNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu, ChinaThe Fourth Affiliated Hospital of Guangzhou Medical University, School of Biomedical Engineering, Guangzhou Medical University, Guangzhou, Guangdong, ChinaGuangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, The NMPA and State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital, Guangzhou Medical University26468, Guangzhou, Guangdong, ChinaThe Fourth Affiliated Hospital of Guangzhou Medical University, School of Biomedical Engineering, Guangzhou Medical University, Guangzhou, Guangdong, ChinaCurrent two-dimensional (2D) cell models for effective drug screening suffer from significant limitations imposed by the lack of realism in the physiological environment. Three-dimensional (3D) organoids models hold immense potential in mimicking the key functions of human organs by overcoming the limitations of traditional 2D cell models. However, current techniques for preparation of 3D organoids models had limitations in reproducibility, scalability, and the ability to closely replicate the complex microenvironment found in vivo. Additionally, traditional 3D cell culture systems often involve lengthy and labor-intensive processes that hinder high-throughput applications necessary for a large-scale drug screening. Advancements in 3D bioprinting technologies offer promising solutions to these challenges by enabling precise spatial control over cell placement and material composition, thereby facilitating the creation of more physiologically relevant organoids than current techniques. This review provides a comprehensive summary of recent advances in 3D bioprinting technologies for creating organoids models, which begins with an introduction to different types of 3D bioprinting techniques (especially focus on volumetric bioprinting (VBP) technique), followed by an overview of bioinks utilized for organoids bioprinting. Moreover, we also introduce the applications of 3D bioprinting organoids in disease models, drug efficiency evaluation and regenerative medicine. Finally, the challenges and possible strategies for the development and clinical translation of 3D bioprinting organoids are concluded.https://doi.org/10.1515/mr-2024-00893d bioprintingvolumetric bioprintingorganoidsbioinksregenerative medicine |
| spellingShingle | Li Zeqing Chen Long Wu Jialin Chen Yikang Zhu Yizhun Li Gang Xie Guoxi Tang Guosheng Xie Maobin A review of 3D bioprinting for organoids Medical Review 3d bioprinting volumetric bioprinting organoids bioinks regenerative medicine |
| title | A review of 3D bioprinting for organoids |
| title_full | A review of 3D bioprinting for organoids |
| title_fullStr | A review of 3D bioprinting for organoids |
| title_full_unstemmed | A review of 3D bioprinting for organoids |
| title_short | A review of 3D bioprinting for organoids |
| title_sort | review of 3d bioprinting for organoids |
| topic | 3d bioprinting volumetric bioprinting organoids bioinks regenerative medicine |
| url | https://doi.org/10.1515/mr-2024-0089 |
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