Genetic relationship analysis and core collection construction of Eucalyptus grandis from Dongmen improved variety base: the largest eucalypt germplasm resource in China
Abstract Background Eucalyptus grandis, which was first comprehensively and systematically introduced to China in the 1980s, is one of the most important fast-growing tree species in the forestry industry. However, to date, no core collection has been selected from the germplasm resources of E. gran...
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BMC
2024-12-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-024-05970-0 |
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| author | Chenhe Li Lei Zhang Zhibang Peng Xia Li Zhao Liu Te Lu Xiangyang Kang Jun Yang |
| author_facet | Chenhe Li Lei Zhang Zhibang Peng Xia Li Zhao Liu Te Lu Xiangyang Kang Jun Yang |
| author_sort | Chenhe Li |
| collection | DOAJ |
| description | Abstract Background Eucalyptus grandis, which was first comprehensively and systematically introduced to China in the 1980s, is one of the most important fast-growing tree species in the forestry industry. However, to date, no core collection has been selected from the germplasm resources of E. grandis based on growth and genetic relationship analysis. Results In the present study, 545 individuals of E. grandis collected from 28 populations across 5 countries were selected for genetic diversity analysis using 16 selected SSR markers. The polymorphism information content (PIC) was employed to assess genetic diversity, yielding a mean value of 0.707. Genetic structure analysis was conducted on 492 individuals from 13 combined populations, revealing three clusters as the most suitable number. Principal coordinate analysis (PCoA) demonstrated that the populations were divided into three major clusters. Additionally, the analysis of molecular variance (AMOVA) indicated that the majority of variation occurred within populations. Conclusions Based on the criteria for screening the core collection, we constructed a population consisting of 158 individuals and created unique fingerprinting codes. These results provide a crucial theoretical foundation for the protection and utilization of germplasm resources of E. grandis in China, which will be helpful in the selection of genetically distant parents for future multigenerational hybridization programs. |
| format | Article |
| id | doaj-art-0f696496eeb14868ab3b9903bd5a760a |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-0f696496eeb14868ab3b9903bd5a760a2024-12-29T12:15:05ZengBMCBMC Plant Biology1471-22292024-12-0124111610.1186/s12870-024-05970-0Genetic relationship analysis and core collection construction of Eucalyptus grandis from Dongmen improved variety base: the largest eucalypt germplasm resource in ChinaChenhe Li0Lei Zhang1Zhibang Peng2Xia Li3Zhao Liu4Te Lu5Xiangyang Kang6Jun Yang7State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry UniversityGuangxi Dongmen Forest FarmGuangxi Dongmen Forest FarmGuangxi Dongmen Forest FarmState Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry UniversityScience and Technology Section, Chifeng Research Institute of Forestry ScienceState Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry UniversityAbstract Background Eucalyptus grandis, which was first comprehensively and systematically introduced to China in the 1980s, is one of the most important fast-growing tree species in the forestry industry. However, to date, no core collection has been selected from the germplasm resources of E. grandis based on growth and genetic relationship analysis. Results In the present study, 545 individuals of E. grandis collected from 28 populations across 5 countries were selected for genetic diversity analysis using 16 selected SSR markers. The polymorphism information content (PIC) was employed to assess genetic diversity, yielding a mean value of 0.707. Genetic structure analysis was conducted on 492 individuals from 13 combined populations, revealing three clusters as the most suitable number. Principal coordinate analysis (PCoA) demonstrated that the populations were divided into three major clusters. Additionally, the analysis of molecular variance (AMOVA) indicated that the majority of variation occurred within populations. Conclusions Based on the criteria for screening the core collection, we constructed a population consisting of 158 individuals and created unique fingerprinting codes. These results provide a crucial theoretical foundation for the protection and utilization of germplasm resources of E. grandis in China, which will be helpful in the selection of genetically distant parents for future multigenerational hybridization programs.https://doi.org/10.1186/s12870-024-05970-0Eucalyptus grandisGenetic diversityPopulation structureSSR markersMolecular fingerprintGuangxi |
| spellingShingle | Chenhe Li Lei Zhang Zhibang Peng Xia Li Zhao Liu Te Lu Xiangyang Kang Jun Yang Genetic relationship analysis and core collection construction of Eucalyptus grandis from Dongmen improved variety base: the largest eucalypt germplasm resource in China BMC Plant Biology Eucalyptus grandis Genetic diversity Population structure SSR markers Molecular fingerprint Guangxi |
| title | Genetic relationship analysis and core collection construction of Eucalyptus grandis from Dongmen improved variety base: the largest eucalypt germplasm resource in China |
| title_full | Genetic relationship analysis and core collection construction of Eucalyptus grandis from Dongmen improved variety base: the largest eucalypt germplasm resource in China |
| title_fullStr | Genetic relationship analysis and core collection construction of Eucalyptus grandis from Dongmen improved variety base: the largest eucalypt germplasm resource in China |
| title_full_unstemmed | Genetic relationship analysis and core collection construction of Eucalyptus grandis from Dongmen improved variety base: the largest eucalypt germplasm resource in China |
| title_short | Genetic relationship analysis and core collection construction of Eucalyptus grandis from Dongmen improved variety base: the largest eucalypt germplasm resource in China |
| title_sort | genetic relationship analysis and core collection construction of eucalyptus grandis from dongmen improved variety base the largest eucalypt germplasm resource in china |
| topic | Eucalyptus grandis Genetic diversity Population structure SSR markers Molecular fingerprint Guangxi |
| url | https://doi.org/10.1186/s12870-024-05970-0 |
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