Intestinal microbiota development in the first week of life of preterm newborns
Objective: This study aimed to evaluate the intestinal microbiota development in the first week of life of preterm newborns (PTNB) treated at a public hospital in a municipality in the Brazilian Northeast. Methods: This is an observational, longitudinal, and descriptive study with 23 PTNBs. Two stoo...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-07-01
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| Series: | Jornal de Pediatria |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0021755725000555 |
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| author | Jessica Santos Passos Costa Heli Vieira Brandão Camilla da Cruz Martins Raquel Guimarães Benevides Jean Carlos Zambrano Contreras Luiz Gustavo Sparvoli Pedro Augusto Ramos Vanzele Carla Romano Taddei Tatiana de Oliveira Vieira Graciete Oliveira Vieira |
| author_facet | Jessica Santos Passos Costa Heli Vieira Brandão Camilla da Cruz Martins Raquel Guimarães Benevides Jean Carlos Zambrano Contreras Luiz Gustavo Sparvoli Pedro Augusto Ramos Vanzele Carla Romano Taddei Tatiana de Oliveira Vieira Graciete Oliveira Vieira |
| author_sort | Jessica Santos Passos Costa |
| collection | DOAJ |
| description | Objective: This study aimed to evaluate the intestinal microbiota development in the first week of life of preterm newborns (PTNB) treated at a public hospital in a municipality in the Brazilian Northeast. Methods: This is an observational, longitudinal, and descriptive study with 23 PTNBs. Two stool samples were collected from each neonate (fasting/meconium and seventh day of life) for stool microbiota analysis by 16S rRNA gene sequencing. The authors analyzed alpha diversity (Chao1, Shannon, and Simpson indices) and principal coordinates of beta diversity. Results: Forty-six stool samples from 23 PTNBs were analyzed at the taxonomic level. Microbiota's development was dynamic with low diversity. The authors observed a statistical association with the genera Enterobacterales, Streptococcus, Bacteroides, Clostridium_sensu_stricto_1, Enterococcus, and Bifidobacterium in the fasting samples when compared to the day-7 samples. The genus Staphylococcus also dominated at both times. Conclusion: Dynamics were observed in the intestinal microbiota development, with an alpha diversity decrease in the stool samples collected at fasting/meconium and on the seventh day of life. |
| format | Article |
| id | doaj-art-0f4a74e0cac84cc9afc3dc6a93e239e4 |
| institution | Kabale University |
| issn | 0021-7557 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Jornal de Pediatria |
| spelling | doaj-art-0f4a74e0cac84cc9afc3dc6a93e239e42025-08-20T03:49:56ZengElsevierJornal de Pediatria0021-75572025-07-01101455156010.1016/j.jped.2025.02.003Intestinal microbiota development in the first week of life of preterm newbornsJessica Santos Passos Costa0Heli Vieira Brandão1Camilla da Cruz Martins2Raquel Guimarães Benevides3Jean Carlos Zambrano Contreras4Luiz Gustavo Sparvoli5Pedro Augusto Ramos Vanzele6Carla Romano Taddei7Tatiana de Oliveira Vieira8Graciete Oliveira Vieira9Universidade Estadual de Feira de Santana (UEFS), Feira de Santana, BA, Brazil; Corresponding author.Universidade Estadual de Feira de Santana (UEFS), Feira de Santana, BA, BrazilUniversidade Estadual de Feira de Santana (UEFS), Feira de Santana, BA, BrazilUniversidade Federal do Ceará (UFC), Fortaleza, CE, BrazilUniversidad de Los Andes, Mérida, VenezuelaUniversidade de São Paulo (USP), São Paulo, SP, BrazilUniversidade de São Paulo (USP), São Paulo, SP, BrazilUniversidade de São Paulo (USP), São Paulo, SP, BrazilUniversidade Estadual de Feira de Santana (UEFS), Feira de Santana, BA, BrazilUniversidade Estadual de Feira de Santana (UEFS), Feira de Santana, BA, BrazilObjective: This study aimed to evaluate the intestinal microbiota development in the first week of life of preterm newborns (PTNB) treated at a public hospital in a municipality in the Brazilian Northeast. Methods: This is an observational, longitudinal, and descriptive study with 23 PTNBs. Two stool samples were collected from each neonate (fasting/meconium and seventh day of life) for stool microbiota analysis by 16S rRNA gene sequencing. The authors analyzed alpha diversity (Chao1, Shannon, and Simpson indices) and principal coordinates of beta diversity. Results: Forty-six stool samples from 23 PTNBs were analyzed at the taxonomic level. Microbiota's development was dynamic with low diversity. The authors observed a statistical association with the genera Enterobacterales, Streptococcus, Bacteroides, Clostridium_sensu_stricto_1, Enterococcus, and Bifidobacterium in the fasting samples when compared to the day-7 samples. The genus Staphylococcus also dominated at both times. Conclusion: Dynamics were observed in the intestinal microbiota development, with an alpha diversity decrease in the stool samples collected at fasting/meconium and on the seventh day of life.http://www.sciencedirect.com/science/article/pii/S0021755725000555Preterm newbornMicrobiota16S RRNA |
| spellingShingle | Jessica Santos Passos Costa Heli Vieira Brandão Camilla da Cruz Martins Raquel Guimarães Benevides Jean Carlos Zambrano Contreras Luiz Gustavo Sparvoli Pedro Augusto Ramos Vanzele Carla Romano Taddei Tatiana de Oliveira Vieira Graciete Oliveira Vieira Intestinal microbiota development in the first week of life of preterm newborns Jornal de Pediatria Preterm newborn Microbiota 16S RRNA |
| title | Intestinal microbiota development in the first week of life of preterm newborns |
| title_full | Intestinal microbiota development in the first week of life of preterm newborns |
| title_fullStr | Intestinal microbiota development in the first week of life of preterm newborns |
| title_full_unstemmed | Intestinal microbiota development in the first week of life of preterm newborns |
| title_short | Intestinal microbiota development in the first week of life of preterm newborns |
| title_sort | intestinal microbiota development in the first week of life of preterm newborns |
| topic | Preterm newborn Microbiota 16S RRNA |
| url | http://www.sciencedirect.com/science/article/pii/S0021755725000555 |
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