Molecular characteristics and antibiotic resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa in Nanning, China
Abstract Purpose This study analyzed antibiotic resistance mechanisms and molecular epidemiology of multidrug-resistant Pseudomonas aeruginosa (MDR-PA), aiming at providing clues for prevention and control of MDR-PA infections. Methods The carbapenemase resistance genes (VIM, IMP, NDM, KPC, GES, OXA...
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2024-11-01
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| Online Access: | https://doi.org/10.1186/s12866-024-03640-7 |
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| author | Xianzhen Wei Mingbo Liu Cuiju Mo Runxian Tan Shan Li Hongjie Liang Meng Li |
| author_facet | Xianzhen Wei Mingbo Liu Cuiju Mo Runxian Tan Shan Li Hongjie Liang Meng Li |
| author_sort | Xianzhen Wei |
| collection | DOAJ |
| description | Abstract Purpose This study analyzed antibiotic resistance mechanisms and molecular epidemiology of multidrug-resistant Pseudomonas aeruginosa (MDR-PA), aiming at providing clues for prevention and control of MDR-PA infections. Methods The carbapenemase resistance genes (VIM, IMP, NDM, KPC, GES, OXA-40) of MDR-PA strains were detected by polymerase chain reaction (PCR) and sequencing. The efflux pump system (MexA, MexC, MexE, MexX), AmpC and OprD2 were detected by real-time fluorescent quantitative PCR (qPCR) in MDR-PA group and sensitive-Pseudomonas aeruginosa (S-PA) group. The homology analysis of MDR-PA strains was performed by multilocus sequence typing (MLST). Results A total of 81 MDR-PA strains were collected from the First Affiliated Hospital of Guangxi Medical University from October 2022 to October 2023. Among the carbapenemase detected, the detection rate of NDM-1 was the highest, with a rate of 34.57% (28/81). MexA had a higher expression in MDR-PA group than that in S-PA group (P<0.0001). 81 MDR-PA strains belonged to 40 different ST types, mainly including ST1971, ST244, ST357 and ST308, and the predominant ST type was ST1971 (34.57%, 28/81). Conclusion The mechanisms of antibiotic resistance of MDR-PA strains mainly were the production of MBLS and higher expression of MexA in our study, and ST1971 was the predominant ST type of MDR-PA strains in our hospital, our findings may assist in prevention and control of MDR-PA infections. |
| format | Article |
| id | doaj-art-0f19091d501c4a5fa14d7cf87551b3b4 |
| institution | Kabale University |
| issn | 1471-2180 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj-art-0f19091d501c4a5fa14d7cf87551b3b42024-11-17T12:12:52ZengBMCBMC Microbiology1471-21802024-11-012411810.1186/s12866-024-03640-7Molecular characteristics and antibiotic resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa in Nanning, ChinaXianzhen Wei0Mingbo Liu1Cuiju Mo2Runxian Tan3Shan Li4Hongjie Liang5Meng Li6Department of Clinical Laboratory, The First People’s Hospital of QinzhouDepartment of Clinical Laboratory, The First People’s Hospital of QinzhouDepartment of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Clinical Laboratory, The First People’s Hospital of QinzhouDepartment of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical UniversityAbstract Purpose This study analyzed antibiotic resistance mechanisms and molecular epidemiology of multidrug-resistant Pseudomonas aeruginosa (MDR-PA), aiming at providing clues for prevention and control of MDR-PA infections. Methods The carbapenemase resistance genes (VIM, IMP, NDM, KPC, GES, OXA-40) of MDR-PA strains were detected by polymerase chain reaction (PCR) and sequencing. The efflux pump system (MexA, MexC, MexE, MexX), AmpC and OprD2 were detected by real-time fluorescent quantitative PCR (qPCR) in MDR-PA group and sensitive-Pseudomonas aeruginosa (S-PA) group. The homology analysis of MDR-PA strains was performed by multilocus sequence typing (MLST). Results A total of 81 MDR-PA strains were collected from the First Affiliated Hospital of Guangxi Medical University from October 2022 to October 2023. Among the carbapenemase detected, the detection rate of NDM-1 was the highest, with a rate of 34.57% (28/81). MexA had a higher expression in MDR-PA group than that in S-PA group (P<0.0001). 81 MDR-PA strains belonged to 40 different ST types, mainly including ST1971, ST244, ST357 and ST308, and the predominant ST type was ST1971 (34.57%, 28/81). Conclusion The mechanisms of antibiotic resistance of MDR-PA strains mainly were the production of MBLS and higher expression of MexA in our study, and ST1971 was the predominant ST type of MDR-PA strains in our hospital, our findings may assist in prevention and control of MDR-PA infections.https://doi.org/10.1186/s12866-024-03640-7Pseudomonas aeruginosaMultidrug-resistantMechanisms of antibiotic resistanceMLST |
| spellingShingle | Xianzhen Wei Mingbo Liu Cuiju Mo Runxian Tan Shan Li Hongjie Liang Meng Li Molecular characteristics and antibiotic resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa in Nanning, China BMC Microbiology Pseudomonas aeruginosa Multidrug-resistant Mechanisms of antibiotic resistance MLST |
| title | Molecular characteristics and antibiotic resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa in Nanning, China |
| title_full | Molecular characteristics and antibiotic resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa in Nanning, China |
| title_fullStr | Molecular characteristics and antibiotic resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa in Nanning, China |
| title_full_unstemmed | Molecular characteristics and antibiotic resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa in Nanning, China |
| title_short | Molecular characteristics and antibiotic resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa in Nanning, China |
| title_sort | molecular characteristics and antibiotic resistance mechanisms of multidrug resistant pseudomonas aeruginosa in nanning china |
| topic | Pseudomonas aeruginosa Multidrug-resistant Mechanisms of antibiotic resistance MLST |
| url | https://doi.org/10.1186/s12866-024-03640-7 |
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