Endoscopic surgery affects the gut microbiota and its metabolism in breast cancer patients
BackgroundDespite the advantages of endoscopic surgery in reducing trauma and enhancing recovery for breast cancer patients, its impact on gut microbiota, which is crucial for health and estrogen metabolism, remains unclear. Further investigation is necessary to fully understand this impact and its...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1481582/full |
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| author | Jingtai Li Fangfang Gao Runwei Li Zhilin Chen Guoping Chen Pingming Fan Guankui Du Guankui Du Guankui Du |
| author_facet | Jingtai Li Fangfang Gao Runwei Li Zhilin Chen Guoping Chen Pingming Fan Guankui Du Guankui Du Guankui Du |
| author_sort | Jingtai Li |
| collection | DOAJ |
| description | BackgroundDespite the advantages of endoscopic surgery in reducing trauma and enhancing recovery for breast cancer patients, its impact on gut microbiota, which is crucial for health and estrogen metabolism, remains unclear. Further investigation is necessary to fully understand this impact and its implications.Materials and methodsBetween June and December 2022, fecal samples were collected from 20 patients who underwent endoscopic surgery. The gut microbiota composition was determined using 16S rRNA sequencing, while the metabolites were analyzed through liquid chromatography-tandem mass spectrometry (LC-MS/MS). Bioinformatics and statistical analyses were employed to identify significant alterations in microbial taxa abundance and to assess intergroup differences. These analyses included t-tests for pairwise comparisons, one-way ANOVA for multiple group comparisons, and chi-square tests for categorical data analysis.ResultsEndoscopic surgery in breast cancer patients subtly changed gut microbiota diversity and composition. Post-surgery, there was a reduction in Lachnospiraceae, Monoglobaceae and Firmicutes to Bacteroides ratios. Shifts in metabolites were also observed, the changed metabolites impacted pathways such as primary bile biosynthesis and Ascorbate and aldarate metabolism, with PE(PGD1/18:1(9Z)) identified as a key differential metabolite that increased post-surgery. Azasetron, tyramine glucuronide, DL-DOPA, phthalide, acetophenazine, aciclovir, creatinine bicarbonate, and 4-oxo-L-proline being associated with distinct bacterial taxa.ConclusionBreast cancer patients undergoing endoscopic surgery experience a shift in their gut microbiota and metabolic profiles. Therefore, postoperative management, with a particular focus on the adjustment of the gut microbiota, is crucial for enhancing patient recovery and health outcomes. |
| format | Article |
| id | doaj-art-0ed76c9f6db74081ba4376e81b40822c |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-0ed76c9f6db74081ba4376e81b40822c2025-01-07T06:49:33ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.14815821481582Endoscopic surgery affects the gut microbiota and its metabolism in breast cancer patientsJingtai Li0Fangfang Gao1Runwei Li2Zhilin Chen3Guoping Chen4Pingming Fan5Guankui Du6Guankui Du7Guankui Du8The First Clinical School of Hainan Medical University, Department of Breast Surgery, The First Affiliated Hospital of Hainan Medical University, Haikou, ChinaThe First Clinical School of Hainan Medical University, Department of Breast Surgery, The First Affiliated Hospital of Hainan Medical University, Haikou, ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaThe First Clinical School of Hainan Medical University, Department of Breast Surgery, The First Affiliated Hospital of Hainan Medical University, Haikou, ChinaThe First Clinical School of Hainan Medical University, Department of Breast Surgery, The First Affiliated Hospital of Hainan Medical University, Haikou, ChinaThe First Clinical School of Hainan Medical University, Department of Breast Surgery, The First Affiliated Hospital of Hainan Medical University, Haikou, ChinaThe First Clinical School of Hainan Medical University, Department of Breast Surgery, The First Affiliated Hospital of Hainan Medical University, Haikou, ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, ChinaDepartment of Biochemistry and Molecular Biology, Hainan Medical University, Haikou, ChinaBackgroundDespite the advantages of endoscopic surgery in reducing trauma and enhancing recovery for breast cancer patients, its impact on gut microbiota, which is crucial for health and estrogen metabolism, remains unclear. Further investigation is necessary to fully understand this impact and its implications.Materials and methodsBetween June and December 2022, fecal samples were collected from 20 patients who underwent endoscopic surgery. The gut microbiota composition was determined using 16S rRNA sequencing, while the metabolites were analyzed through liquid chromatography-tandem mass spectrometry (LC-MS/MS). Bioinformatics and statistical analyses were employed to identify significant alterations in microbial taxa abundance and to assess intergroup differences. These analyses included t-tests for pairwise comparisons, one-way ANOVA for multiple group comparisons, and chi-square tests for categorical data analysis.ResultsEndoscopic surgery in breast cancer patients subtly changed gut microbiota diversity and composition. Post-surgery, there was a reduction in Lachnospiraceae, Monoglobaceae and Firmicutes to Bacteroides ratios. Shifts in metabolites were also observed, the changed metabolites impacted pathways such as primary bile biosynthesis and Ascorbate and aldarate metabolism, with PE(PGD1/18:1(9Z)) identified as a key differential metabolite that increased post-surgery. Azasetron, tyramine glucuronide, DL-DOPA, phthalide, acetophenazine, aciclovir, creatinine bicarbonate, and 4-oxo-L-proline being associated with distinct bacterial taxa.ConclusionBreast cancer patients undergoing endoscopic surgery experience a shift in their gut microbiota and metabolic profiles. Therefore, postoperative management, with a particular focus on the adjustment of the gut microbiota, is crucial for enhancing patient recovery and health outcomes.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1481582/fullbreast cancerendoscopic surgerygut microbiotametabolites16S rRNA |
| spellingShingle | Jingtai Li Fangfang Gao Runwei Li Zhilin Chen Guoping Chen Pingming Fan Guankui Du Guankui Du Guankui Du Endoscopic surgery affects the gut microbiota and its metabolism in breast cancer patients Frontiers in Microbiology breast cancer endoscopic surgery gut microbiota metabolites 16S rRNA |
| title | Endoscopic surgery affects the gut microbiota and its metabolism in breast cancer patients |
| title_full | Endoscopic surgery affects the gut microbiota and its metabolism in breast cancer patients |
| title_fullStr | Endoscopic surgery affects the gut microbiota and its metabolism in breast cancer patients |
| title_full_unstemmed | Endoscopic surgery affects the gut microbiota and its metabolism in breast cancer patients |
| title_short | Endoscopic surgery affects the gut microbiota and its metabolism in breast cancer patients |
| title_sort | endoscopic surgery affects the gut microbiota and its metabolism in breast cancer patients |
| topic | breast cancer endoscopic surgery gut microbiota metabolites 16S rRNA |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1481582/full |
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