Protocol for isolating antigen-specific monoclonal antibodies from immunized mice utilizing the Beacon platform

Protocol for isolating antigen-specific monoclonal antibodies from immunized mice utilizing the Beacon platform

Summary: Isolation of antigen-specific plasma B cells had been challenging until the recent arrival of the Beacon platform. Leveraging light-sorting technology, Beacon can perform high-throughput screening of plasma B cells on a chip to sort single cells with the desired antigen specificity. Here, w...

Full description

Saved in:
Bibliographic Details
Main Authors: Meilan Fu, Xiangwei Wang, Shubing Tang, Shimeng Bai, Longfei Ding, Yangyang Hu, Kangli Cao, Tianhan Yang, Chen Zhao, Xiaoyan Zhang, Jianqing Xu
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
Cell isolation
Single Cell
Sequencing
Antibody
Protein expression and purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724005549
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

http://www.sciencedirect.com/science/article/pii/S2666166724005549

Similar Items