First VIM-producing representative of Pseudomonas putida group from the largest Bulgarian hospital
Here we describe the first detected VIM-2-producing representative of Pseudomonas putida group – Pseudomonas kurunegalensis from the largest Bulgarian hospital – St George University Hospital in Plovdiv. A 59-year-old female patient with right-sided lung abscess was hospitalized in the Second Cl...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pensoft Publishers
2024-12-01
|
| Series: | Folia Medica |
| Online Access: | https://foliamedica.bg/article/129478/download/pdf/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841556472993939456 |
|---|---|
| author | Atanaska Petrova Ivan N. Ivanov Liubomir Paunov Angel Uchikov Ivan Stoikov Todor Kantardjiev Marianna Murdjeva |
| author_facet | Atanaska Petrova Ivan N. Ivanov Liubomir Paunov Angel Uchikov Ivan Stoikov Todor Kantardjiev Marianna Murdjeva |
| author_sort | Atanaska Petrova |
| collection | DOAJ |
| description | Here we describe the first detected VIM-2-producing representative of Pseudomonas putida group – Pseudomonas kurunegalensis from the largest Bulgarian hospital – St George University Hospital in Plovdiv. A 59-year-old female patient with right-sided lung abscess was hospitalized in the Second Clinic of Thoracoabdominal Surgery. She was repeatedly treated for pulmonary infections. Punctate from the abscess cavity was taken for microbiological investigation. Identification process and antimicrobial susceptibility were performed by Vitek 2. The species group P. putida was confirmed with MALDI-TOF system and whole genome sequencing defined it as P. kurunegalensis. Antibiotic susceptibility testing revealed susceptibility only to tobramycin and colistin. All phenotypic tests for carbapenemase and metallo-beta-lactamase (MBL) production were positive. Multiplex PCR was performed to search for nine common carbapenemase encoding genes whereas the variable region of the integron was determined by DNA sequencing. Molecular assays confirmed the presence of blaVIM-2 located within a typical Class I integron including also an aacA29b aminoglycoside N(6’)-acetyltransferase casette. Despite P. putida not being a common pathogen, it still could survive in hospital conditions causing difficult-to-treat infections and becoming a source of resistant genes, including MBL-encoding genes. |
| format | Article |
| id | doaj-art-0e699386dd434fad98cdb2ee4f15f00f |
| institution | Kabale University |
| issn | 1314-2143 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Folia Medica |
| spelling | doaj-art-0e699386dd434fad98cdb2ee4f15f00f2025-01-07T08:30:13ZengPensoft PublishersFolia Medica1314-21432024-12-0166690591010.3897/folmed.66.e129478129478First VIM-producing representative of Pseudomonas putida group from the largest Bulgarian hospitalAtanaska Petrova0Ivan N. Ivanov1Liubomir Paunov2Angel Uchikov3Ivan Stoikov4Todor Kantardjiev5Marianna Murdjeva6St George University HospitalNCIPD-SofiaMedical University of PlovdivMedical University of PlovdivNCIPD-SofiaNCIPD-SofiaSt George University HospitalHere we describe the first detected VIM-2-producing representative of Pseudomonas putida group – Pseudomonas kurunegalensis from the largest Bulgarian hospital – St George University Hospital in Plovdiv. A 59-year-old female patient with right-sided lung abscess was hospitalized in the Second Clinic of Thoracoabdominal Surgery. She was repeatedly treated for pulmonary infections. Punctate from the abscess cavity was taken for microbiological investigation. Identification process and antimicrobial susceptibility were performed by Vitek 2. The species group P. putida was confirmed with MALDI-TOF system and whole genome sequencing defined it as P. kurunegalensis. Antibiotic susceptibility testing revealed susceptibility only to tobramycin and colistin. All phenotypic tests for carbapenemase and metallo-beta-lactamase (MBL) production were positive. Multiplex PCR was performed to search for nine common carbapenemase encoding genes whereas the variable region of the integron was determined by DNA sequencing. Molecular assays confirmed the presence of blaVIM-2 located within a typical Class I integron including also an aacA29b aminoglycoside N(6’)-acetyltransferase casette. Despite P. putida not being a common pathogen, it still could survive in hospital conditions causing difficult-to-treat infections and becoming a source of resistant genes, including MBL-encoding genes.https://foliamedica.bg/article/129478/download/pdf/ |
| spellingShingle | Atanaska Petrova Ivan N. Ivanov Liubomir Paunov Angel Uchikov Ivan Stoikov Todor Kantardjiev Marianna Murdjeva First VIM-producing representative of Pseudomonas putida group from the largest Bulgarian hospital Folia Medica |
| title | First VIM-producing representative of Pseudomonas putida group from the largest Bulgarian hospital |
| title_full | First VIM-producing representative of Pseudomonas putida group from the largest Bulgarian hospital |
| title_fullStr | First VIM-producing representative of Pseudomonas putida group from the largest Bulgarian hospital |
| title_full_unstemmed | First VIM-producing representative of Pseudomonas putida group from the largest Bulgarian hospital |
| title_short | First VIM-producing representative of Pseudomonas putida group from the largest Bulgarian hospital |
| title_sort | first vim producing representative of pseudomonas putida group from the largest bulgarian hospital |
| url | https://foliamedica.bg/article/129478/download/pdf/ |
| work_keys_str_mv | AT atanaskapetrova firstvimproducingrepresentativeofpseudomonasputidagroupfromthelargestbulgarianhospital AT ivannivanov firstvimproducingrepresentativeofpseudomonasputidagroupfromthelargestbulgarianhospital AT liubomirpaunov firstvimproducingrepresentativeofpseudomonasputidagroupfromthelargestbulgarianhospital AT angeluchikov firstvimproducingrepresentativeofpseudomonasputidagroupfromthelargestbulgarianhospital AT ivanstoikov firstvimproducingrepresentativeofpseudomonasputidagroupfromthelargestbulgarianhospital AT todorkantardjiev firstvimproducingrepresentativeofpseudomonasputidagroupfromthelargestbulgarianhospital AT mariannamurdjeva firstvimproducingrepresentativeofpseudomonasputidagroupfromthelargestbulgarianhospital |