Genome-Wide Identification and Analysis of GATA Gene Family in <i>Dendrobium officinale</i> Under Methyl Jasmonate and Salt Stress
<i>Dendrobium officinale,</i> which was rich in bioactive compounds such as polysaccharides, alkaloids, amino acids, and flavonoids, had significant medicinal value and ability to resist stresses. Studies had demonstrated that <i>GATA</i> genes were one of the crucial regulat...
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2025-05-01
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| author | Zhangting Xu Feixiong Zheng Xiaoji Deng Yiming Sun Zhenming Yu Xiaoxia Shen |
| author_facet | Zhangting Xu Feixiong Zheng Xiaoji Deng Yiming Sun Zhenming Yu Xiaoxia Shen |
| author_sort | Zhangting Xu |
| collection | DOAJ |
| description | <i>Dendrobium officinale,</i> which was rich in bioactive compounds such as polysaccharides, alkaloids, amino acids, and flavonoids, had significant medicinal value and ability to resist stresses. Studies had demonstrated that <i>GATA</i> genes were one of the crucial regulators in controlling plant development and growth and stress response. Genome-wide identification and characterization of the 18 <i>DoGATA</i> genes were displayed. According to phylogenetic relationships, the <i>DoGATA</i> family genes were divided into 4 groups and the conserved motifs of <i>DoGATA1</i>–<i>DoGATA18</i> within the same group were similar. All <i>DoGATA</i> genes were localized in the nucleus and randomly mapped on 10 chromosomes. The GATA genes in <i>D. officinale</i> experienced one pair of tandem duplication and 4 pairs of segment duplications to expand the family genes. Additionally, we found that the 2000 bp upstream promoter region of the <i>DoGATA</i> genes harbored 23 types of <i>cis</i>-acting elements that were categorized into plant growth and development, phytohormone responsiveness, and stress responsiveness. <i>DoGATA1</i>–<i>DoGATA18</i> were diversely expressed across different tissues (root, leaf, stem, flower), exposed to salt stress, and following MeJA treatment. Co-expression analysis between <i>DoGATA</i> and enzyme-encoding genes involved in the biosynthesis of flavone showed that <i>DoCHI</i> (LOC110104562) and <i>DoGTMT</i> (LOC110098370) may be potential downstream targets of <i>DoGATA16</i> to regulate flavonoid biosynthesis to adapt to salt stress. Furthermore, we confirmed that <i>DoGATA16</i> may act as a key member to resist stress. The collective findings of this study shed light on the function of <i>GATA</i> genes and molecular breeding of <i>D. officinale</i>. |
| format | Article |
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| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-0e3e7ceb185f43edb7d89a8f955f7cdd2025-08-20T03:46:49ZengMDPI AGPlants2223-77472025-05-011411157610.3390/plants14111576Genome-Wide Identification and Analysis of GATA Gene Family in <i>Dendrobium officinale</i> Under Methyl Jasmonate and Salt StressZhangting Xu0Feixiong Zheng1Xiaoji Deng2Yiming Sun3Zhenming Yu4Xiaoxia Shen5School of Pharmaceutical Sciences, Zhejiang Engineering Research Center for Chinese Medicinal Plant Essential Oils, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Engineering Research Center for Chinese Medicinal Plant Essential Oils, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Engineering Research Center for Chinese Medicinal Plant Essential Oils, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Engineering Research Center for Chinese Medicinal Plant Essential Oils, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Engineering Research Center for Chinese Medicinal Plant Essential Oils, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Engineering Research Center for Chinese Medicinal Plant Essential Oils, Zhejiang Chinese Medical University, Hangzhou 310053, China<i>Dendrobium officinale,</i> which was rich in bioactive compounds such as polysaccharides, alkaloids, amino acids, and flavonoids, had significant medicinal value and ability to resist stresses. Studies had demonstrated that <i>GATA</i> genes were one of the crucial regulators in controlling plant development and growth and stress response. Genome-wide identification and characterization of the 18 <i>DoGATA</i> genes were displayed. According to phylogenetic relationships, the <i>DoGATA</i> family genes were divided into 4 groups and the conserved motifs of <i>DoGATA1</i>–<i>DoGATA18</i> within the same group were similar. All <i>DoGATA</i> genes were localized in the nucleus and randomly mapped on 10 chromosomes. The GATA genes in <i>D. officinale</i> experienced one pair of tandem duplication and 4 pairs of segment duplications to expand the family genes. Additionally, we found that the 2000 bp upstream promoter region of the <i>DoGATA</i> genes harbored 23 types of <i>cis</i>-acting elements that were categorized into plant growth and development, phytohormone responsiveness, and stress responsiveness. <i>DoGATA1</i>–<i>DoGATA18</i> were diversely expressed across different tissues (root, leaf, stem, flower), exposed to salt stress, and following MeJA treatment. Co-expression analysis between <i>DoGATA</i> and enzyme-encoding genes involved in the biosynthesis of flavone showed that <i>DoCHI</i> (LOC110104562) and <i>DoGTMT</i> (LOC110098370) may be potential downstream targets of <i>DoGATA16</i> to regulate flavonoid biosynthesis to adapt to salt stress. Furthermore, we confirmed that <i>DoGATA16</i> may act as a key member to resist stress. The collective findings of this study shed light on the function of <i>GATA</i> genes and molecular breeding of <i>D. officinale</i>.https://www.mdpi.com/2223-7747/14/11/1576<i>Dendrobium officinale</i>GATAexpression analysisflavonoidstress |
| spellingShingle | Zhangting Xu Feixiong Zheng Xiaoji Deng Yiming Sun Zhenming Yu Xiaoxia Shen Genome-Wide Identification and Analysis of GATA Gene Family in <i>Dendrobium officinale</i> Under Methyl Jasmonate and Salt Stress Plants <i>Dendrobium officinale</i> GATA expression analysis flavonoid stress |
| title | Genome-Wide Identification and Analysis of GATA Gene Family in <i>Dendrobium officinale</i> Under Methyl Jasmonate and Salt Stress |
| title_full | Genome-Wide Identification and Analysis of GATA Gene Family in <i>Dendrobium officinale</i> Under Methyl Jasmonate and Salt Stress |
| title_fullStr | Genome-Wide Identification and Analysis of GATA Gene Family in <i>Dendrobium officinale</i> Under Methyl Jasmonate and Salt Stress |
| title_full_unstemmed | Genome-Wide Identification and Analysis of GATA Gene Family in <i>Dendrobium officinale</i> Under Methyl Jasmonate and Salt Stress |
| title_short | Genome-Wide Identification and Analysis of GATA Gene Family in <i>Dendrobium officinale</i> Under Methyl Jasmonate and Salt Stress |
| title_sort | genome wide identification and analysis of gata gene family in i dendrobium officinale i under methyl jasmonate and salt stress |
| topic | <i>Dendrobium officinale</i> GATA expression analysis flavonoid stress |
| url | https://www.mdpi.com/2223-7747/14/11/1576 |
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