Facile fabrication of chitosan/hyaluronic acid hydrogel-based wound closure material Co-loaded with gold nanoparticles and fibroblast growth factor to improve anti-microbial and healing efficiency in diabetic wound healing and nursing care
Generally, diabetic wounds heal very slowly and inefficiently with an increasing risk of infections. Recent nanotechnology and biomaterial advances elaborate developed multi-functional hydrogels and nanoparticles offer promising solutions to accelerate wound healing for diabetic patients. This resea...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
|
| Series: | Regenerative Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320424001810 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846148860535111680 |
|---|---|
| author | Xin Liu Shengwei Peng Yongju Pei Yuanyuan Huo Yadi Zong Jianwei Ren Jing Zhao |
| author_facet | Xin Liu Shengwei Peng Yongju Pei Yuanyuan Huo Yadi Zong Jianwei Ren Jing Zhao |
| author_sort | Xin Liu |
| collection | DOAJ |
| description | Generally, diabetic wounds heal very slowly and inefficiently with an increasing risk of infections. Recent nanotechnology and biomaterial advances elaborate developed multi-functional hydrogels and nanoparticles offer promising solutions to accelerate wound healing for diabetic patients. This research work demonstrates to use of solvent diffusion method to develop hydrogel nanocomposites composed of chitosan (CS), hyaluronic acid (HA), gold (Au), and fibroblast growth factors (FGF). The biological analysis of nanocomposites exhibited enhanced wound healing efficiency by incorporating bioactive molecules like FGF and bioactive Au nanoparticles. In vitro, cell compatibility analysis (MTT assay) of prepared hydrogel nanocomposites was studied on fibroblast cell lines NIH-3T3-L1 and L929 and exhibited greater cell survival ability (>90 %), cell proliferation and migration ability, which demonstrated the suitability of nanocomposite for wound healing treatment. In vitro, anti-bacterial analyses established that FGF-Au@CS/HA has strong antibacterial effectiveness against gram-positive and gram-negative pathogens. The observation of the present research revealed that prepared FGF-Au@CS/HA hydrogel composites could be a suitable biomaterial for diabetic wound care, potentially improving its antibacterial and healing efficacies. |
| format | Article |
| id | doaj-art-0dc6505ac2e847ed9d7825479dab8baf |
| institution | Kabale University |
| issn | 2352-3204 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj-art-0dc6505ac2e847ed9d7825479dab8baf2024-11-30T07:11:06ZengElsevierRegenerative Therapy2352-32042024-06-012610181029Facile fabrication of chitosan/hyaluronic acid hydrogel-based wound closure material Co-loaded with gold nanoparticles and fibroblast growth factor to improve anti-microbial and healing efficiency in diabetic wound healing and nursing careXin Liu0Shengwei Peng1Yongju Pei2Yuanyuan Huo3Yadi Zong4Jianwei Ren5Jing Zhao6Department of Respiratory Intensive Care Unit, Henan Provincial Key Medicine Laboratory of Nursing, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan, 450003, ChinaDepartment of Respiratory Intensive Care Unit, Henan Provincial Key Medicine Laboratory of Nursing, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan, 450003, ChinaDepartment of Respiratory Intensive Care Unit, Henan Provincial Key Medicine Laboratory of Nursing, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan, 450003, ChinaDepartment of Respiratory Intensive Care Unit, Henan Provincial Key Medicine Laboratory of Nursing, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan, 450003, ChinaDepartment of Pediatric Surgery, Henan Provincial Key Medicine Laboratory of Nursing, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan, 450003, ChinaDepartment of Respiratory Intensive Care Unit, Henan Provincial Key Medicine Laboratory of Nursing, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan, 450003, ChinaDepartment of Respiratory Intensive Care Unit, Henan Provincial Key Medicine Laboratory of Nursing, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan, 450003, China; Corresponding author. NO.7, Jingwu Road, Zhengzhou 450003, China.Generally, diabetic wounds heal very slowly and inefficiently with an increasing risk of infections. Recent nanotechnology and biomaterial advances elaborate developed multi-functional hydrogels and nanoparticles offer promising solutions to accelerate wound healing for diabetic patients. This research work demonstrates to use of solvent diffusion method to develop hydrogel nanocomposites composed of chitosan (CS), hyaluronic acid (HA), gold (Au), and fibroblast growth factors (FGF). The biological analysis of nanocomposites exhibited enhanced wound healing efficiency by incorporating bioactive molecules like FGF and bioactive Au nanoparticles. In vitro, cell compatibility analysis (MTT assay) of prepared hydrogel nanocomposites was studied on fibroblast cell lines NIH-3T3-L1 and L929 and exhibited greater cell survival ability (>90 %), cell proliferation and migration ability, which demonstrated the suitability of nanocomposite for wound healing treatment. In vitro, anti-bacterial analyses established that FGF-Au@CS/HA has strong antibacterial effectiveness against gram-positive and gram-negative pathogens. The observation of the present research revealed that prepared FGF-Au@CS/HA hydrogel composites could be a suitable biomaterial for diabetic wound care, potentially improving its antibacterial and healing efficacies.http://www.sciencedirect.com/science/article/pii/S2352320424001810ChitosanDiabetic wound healingFibroblast growth factorsGold nanoparticlesHyaluronic acid |
| spellingShingle | Xin Liu Shengwei Peng Yongju Pei Yuanyuan Huo Yadi Zong Jianwei Ren Jing Zhao Facile fabrication of chitosan/hyaluronic acid hydrogel-based wound closure material Co-loaded with gold nanoparticles and fibroblast growth factor to improve anti-microbial and healing efficiency in diabetic wound healing and nursing care Regenerative Therapy Chitosan Diabetic wound healing Fibroblast growth factors Gold nanoparticles Hyaluronic acid |
| title | Facile fabrication of chitosan/hyaluronic acid hydrogel-based wound closure material Co-loaded with gold nanoparticles and fibroblast growth factor to improve anti-microbial and healing efficiency in diabetic wound healing and nursing care |
| title_full | Facile fabrication of chitosan/hyaluronic acid hydrogel-based wound closure material Co-loaded with gold nanoparticles and fibroblast growth factor to improve anti-microbial and healing efficiency in diabetic wound healing and nursing care |
| title_fullStr | Facile fabrication of chitosan/hyaluronic acid hydrogel-based wound closure material Co-loaded with gold nanoparticles and fibroblast growth factor to improve anti-microbial and healing efficiency in diabetic wound healing and nursing care |
| title_full_unstemmed | Facile fabrication of chitosan/hyaluronic acid hydrogel-based wound closure material Co-loaded with gold nanoparticles and fibroblast growth factor to improve anti-microbial and healing efficiency in diabetic wound healing and nursing care |
| title_short | Facile fabrication of chitosan/hyaluronic acid hydrogel-based wound closure material Co-loaded with gold nanoparticles and fibroblast growth factor to improve anti-microbial and healing efficiency in diabetic wound healing and nursing care |
| title_sort | facile fabrication of chitosan hyaluronic acid hydrogel based wound closure material co loaded with gold nanoparticles and fibroblast growth factor to improve anti microbial and healing efficiency in diabetic wound healing and nursing care |
| topic | Chitosan Diabetic wound healing Fibroblast growth factors Gold nanoparticles Hyaluronic acid |
| url | http://www.sciencedirect.com/science/article/pii/S2352320424001810 |
| work_keys_str_mv | AT xinliu facilefabricationofchitosanhyaluronicacidhydrogelbasedwoundclosurematerialcoloadedwithgoldnanoparticlesandfibroblastgrowthfactortoimproveantimicrobialandhealingefficiencyindiabeticwoundhealingandnursingcare AT shengweipeng facilefabricationofchitosanhyaluronicacidhydrogelbasedwoundclosurematerialcoloadedwithgoldnanoparticlesandfibroblastgrowthfactortoimproveantimicrobialandhealingefficiencyindiabeticwoundhealingandnursingcare AT yongjupei facilefabricationofchitosanhyaluronicacidhydrogelbasedwoundclosurematerialcoloadedwithgoldnanoparticlesandfibroblastgrowthfactortoimproveantimicrobialandhealingefficiencyindiabeticwoundhealingandnursingcare AT yuanyuanhuo facilefabricationofchitosanhyaluronicacidhydrogelbasedwoundclosurematerialcoloadedwithgoldnanoparticlesandfibroblastgrowthfactortoimproveantimicrobialandhealingefficiencyindiabeticwoundhealingandnursingcare AT yadizong facilefabricationofchitosanhyaluronicacidhydrogelbasedwoundclosurematerialcoloadedwithgoldnanoparticlesandfibroblastgrowthfactortoimproveantimicrobialandhealingefficiencyindiabeticwoundhealingandnursingcare AT jianweiren facilefabricationofchitosanhyaluronicacidhydrogelbasedwoundclosurematerialcoloadedwithgoldnanoparticlesandfibroblastgrowthfactortoimproveantimicrobialandhealingefficiencyindiabeticwoundhealingandnursingcare AT jingzhao facilefabricationofchitosanhyaluronicacidhydrogelbasedwoundclosurematerialcoloadedwithgoldnanoparticlesandfibroblastgrowthfactortoimproveantimicrobialandhealingefficiencyindiabeticwoundhealingandnursingcare |