Detection and quantification of Brucella abortus DNA in water buffaloes (bubalus bubalis) using droplet digital polymerase chain reaction

Detection and quantification of Brucella abortus DNA in water buffaloes (bubalus bubalis) using droplet digital polymerase chain reaction

Brucellosis represents a major public health concern worldwide. Human transmission is mainly due to the consumption of unpasteurized milk and dairy products of infected animals. The gold standard for the diagnosis of Brucella spp in ruminants is the bacterial isolation, but it is time-consuming. Pol...

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Bibliographic Details
Main Authors: Giovanna Fusco, Lorena Cardillo, Ornella Valvini, Alessia Pucciarelli, Gerardo Picazio, Anna Cerrone, Michele Napoletano, Roberta Pellicanò, Maria Ottaiano, Claudio de Martinis, Francesca De Falco, Anna Cutarelli, Emanuela Sannino, Giorgia Borriello, Manuela Tittarelli, Sante Roperto, Esterina De Carlo
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Veterinary Quarterly
Subjects:
Brucella abortus
droplet digital-PCR
real-time PCR
bacterial culture
water buffalo
Sensitivity
Online Access:https://www.tandfonline.com/doi/10.1080/01652176.2024.2390944
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