The Potential of some Plant Extracts as Radical Scavengers and Dipeptidyl Peptidase-4 Inhibitors

Seeds, beans, leaves, fruit peel and seeds of five plants (Ferula assa-foetida, Coffea robusta, Olea europaea, Punica granatum and Vitis vinifera, respectively) were extracted with four solvents (distilled water, 80% methanol, 80% acetone and a mixed solvent that included methanol, ethanol, acetone...

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Main Author: Malik et al.
Format: Article
Language:English
Published: University of Baghdad, College of Science for Women 2019-03-01
Series:مجلة بغداد للعلوم
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Online Access:http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3229
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author Malik et al.
author_facet Malik et al.
author_sort Malik et al.
collection DOAJ
description Seeds, beans, leaves, fruit peel and seeds of five plants (Ferula assa-foetida, Coffea robusta, Olea europaea, Punica granatum and Vitis vinifera, respectively) were extracted with four solvents (distilled water, 80% methanol, 80% acetone and a mixed solvent that included methanol, ethanol, acetone and n-butanol at proportions 7:1:1:1). Such manipulation yielded 20 extracts, which were phytochemically analyzed for total polyphenols (TP) and flavonoids (TF). The DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity (RSA) and DPP-4 (dipeptidyl peptidase-4) relative inhibition activity (RIA) were also assessed for each extract. The results revealed that mixed solvent extract of V. vinifera seeds showed the highest content of TP (194.8 ± 2.5 mg Gallic acid equivalent/g dry mass), while methanol extract of V. vinifera seeds recorded the highest content of TF (75.9 ± 0.3 mg catechin equivalent/g dry mass). The C. robusta bean aqueous extract was remarkable in scoring the highest DPPH RSA (87.2 ± 1.2%), while O. europaea leaf methanol extract had the highest DPP-4 RIA (86.4 ± 0.8%). In conclusion, the importance of natural products as radical scavengers and DPP-4 inhibitors is encouraged, and such biological effects were dependent on the plant species and the solvent of extraction. C. robusta beans are suggested to have a prominent RSA, while O. europaea leaves are recommended to be a target for investigations involved in the development of anti-T2DM therapies.
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spelling doaj-art-0da511f821814959a43cde4083ee91272025-08-20T03:58:10ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862019-03-0116110.21123/bsj.16.1.(suppl.).0162The Potential of some Plant Extracts as Radical Scavengers and Dipeptidyl Peptidase-4 InhibitorsMalik et al.Seeds, beans, leaves, fruit peel and seeds of five plants (Ferula assa-foetida, Coffea robusta, Olea europaea, Punica granatum and Vitis vinifera, respectively) were extracted with four solvents (distilled water, 80% methanol, 80% acetone and a mixed solvent that included methanol, ethanol, acetone and n-butanol at proportions 7:1:1:1). Such manipulation yielded 20 extracts, which were phytochemically analyzed for total polyphenols (TP) and flavonoids (TF). The DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity (RSA) and DPP-4 (dipeptidyl peptidase-4) relative inhibition activity (RIA) were also assessed for each extract. The results revealed that mixed solvent extract of V. vinifera seeds showed the highest content of TP (194.8 ± 2.5 mg Gallic acid equivalent/g dry mass), while methanol extract of V. vinifera seeds recorded the highest content of TF (75.9 ± 0.3 mg catechin equivalent/g dry mass). The C. robusta bean aqueous extract was remarkable in scoring the highest DPPH RSA (87.2 ± 1.2%), while O. europaea leaf methanol extract had the highest DPP-4 RIA (86.4 ± 0.8%). In conclusion, the importance of natural products as radical scavengers and DPP-4 inhibitors is encouraged, and such biological effects were dependent on the plant species and the solvent of extraction. C. robusta beans are suggested to have a prominent RSA, while O. europaea leaves are recommended to be a target for investigations involved in the development of anti-T2DM therapies.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3229Coffea robusta, DPPH, DPP-4 inhibitors, Ferula assa-foetida, Olea europaea, Punica granatum, Vitis vinifera.
spellingShingle Malik et al.
The Potential of some Plant Extracts as Radical Scavengers and Dipeptidyl Peptidase-4 Inhibitors
مجلة بغداد للعلوم
Coffea robusta, DPPH, DPP-4 inhibitors, Ferula assa-foetida, Olea europaea, Punica granatum, Vitis vinifera.
title The Potential of some Plant Extracts as Radical Scavengers and Dipeptidyl Peptidase-4 Inhibitors
title_full The Potential of some Plant Extracts as Radical Scavengers and Dipeptidyl Peptidase-4 Inhibitors
title_fullStr The Potential of some Plant Extracts as Radical Scavengers and Dipeptidyl Peptidase-4 Inhibitors
title_full_unstemmed The Potential of some Plant Extracts as Radical Scavengers and Dipeptidyl Peptidase-4 Inhibitors
title_short The Potential of some Plant Extracts as Radical Scavengers and Dipeptidyl Peptidase-4 Inhibitors
title_sort potential of some plant extracts as radical scavengers and dipeptidyl peptidase 4 inhibitors
topic Coffea robusta, DPPH, DPP-4 inhibitors, Ferula assa-foetida, Olea europaea, Punica granatum, Vitis vinifera.
url http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3229
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