Role of the two-component system AmgRS in early resistance of Pseudomonas aeruginosa to cinnamaldehyde
ABSTRACT Exposure of Pseudomonas aeruginosa to cinnamaldehyde (CNA), a natural electrophilic antimicrobial often used as self-medication to treat mild infections, triggers overproduction of the MexAB-OprM efflux system, leading to multidrug resistance. In this study, we demonstrate that CNA exposure...
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American Society for Microbiology
2025-01-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.01699-24 |
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| author | Eline Dubois Vladimir Spasovski Patrick Plésiat Catherine Llanes |
| author_facet | Eline Dubois Vladimir Spasovski Patrick Plésiat Catherine Llanes |
| author_sort | Eline Dubois |
| collection | DOAJ |
| description | ABSTRACT Exposure of Pseudomonas aeruginosa to cinnamaldehyde (CNA), a natural electrophilic antimicrobial often used as self-medication to treat mild infections, triggers overproduction of the MexAB-OprM efflux system, leading to multidrug resistance. In this study, we demonstrate that CNA exposure induces expression of genes regulated by the two-component system AmgRS. AmgRS activates MexAB-OprM production, independent of repressors MexR and NalD. In addition to the essential role played by the NalC-ArmR pathway in this adaptive process, AmgRS is critical for the survival of P. aeruginosa challenged with CNA. Altogether, these data suggest that efflux-dependent and -independent mechanisms are activated in the early phase of CNA exposure, allowing for progressive enzymatic reduction of the biocide to non-toxic cinnamic alcohol.IMPORTANCEExposure of Pseudomonas aeruginosa to cinnamaldehyde (CNA), an antimicrobial used in self-medication, induces overproduction of the MexAB-OprM efflux system, leading to multidrug resistance. Our study demonstrates that the AmgRS two-component system aids in the survival of P. aeruginosa strain PA14 under CNA exposure through both MexAB-OprM-dependent and -independent mechanisms until the enzymatic reduction of CNA into the less toxic cinnamic alcohol. This discovery highlights the pivotal role of AmgRS in mediating defense against aldehyde biocides, emphasizing its significance in the persistence of P. aeruginosa, a pathogen associated with hospital-acquired infections and cystic fibrosis, and underscores the potential impact on clinical treatment strategies. |
| format | Article |
| id | doaj-art-0d7b2b683fee4c7891c69ee72ebbd523 |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | American Society for Microbiology |
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| series | Microbiology Spectrum |
| spelling | doaj-art-0d7b2b683fee4c7891c69ee72ebbd5232025-01-07T14:05:18ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-01-0113110.1128/spectrum.01699-24Role of the two-component system AmgRS in early resistance of Pseudomonas aeruginosa to cinnamaldehydeEline Dubois0Vladimir Spasovski1Patrick Plésiat2Catherine Llanes3UMR CNRS 6249 Chrono-Environnement, UFR Santé, Université Bourgogne Franche-Comté, Besançon, FranceUMR CNRS 6249 Chrono-Environnement, UFR Santé, Université Bourgogne Franche-Comté, Besançon, FranceUMR CNRS 6249 Chrono-Environnement, UFR Santé, Université Bourgogne Franche-Comté, Besançon, FranceUMR CNRS 6249 Chrono-Environnement, UFR Santé, Université Bourgogne Franche-Comté, Besançon, FranceABSTRACT Exposure of Pseudomonas aeruginosa to cinnamaldehyde (CNA), a natural electrophilic antimicrobial often used as self-medication to treat mild infections, triggers overproduction of the MexAB-OprM efflux system, leading to multidrug resistance. In this study, we demonstrate that CNA exposure induces expression of genes regulated by the two-component system AmgRS. AmgRS activates MexAB-OprM production, independent of repressors MexR and NalD. In addition to the essential role played by the NalC-ArmR pathway in this adaptive process, AmgRS is critical for the survival of P. aeruginosa challenged with CNA. Altogether, these data suggest that efflux-dependent and -independent mechanisms are activated in the early phase of CNA exposure, allowing for progressive enzymatic reduction of the biocide to non-toxic cinnamic alcohol.IMPORTANCEExposure of Pseudomonas aeruginosa to cinnamaldehyde (CNA), an antimicrobial used in self-medication, induces overproduction of the MexAB-OprM efflux system, leading to multidrug resistance. Our study demonstrates that the AmgRS two-component system aids in the survival of P. aeruginosa strain PA14 under CNA exposure through both MexAB-OprM-dependent and -independent mechanisms until the enzymatic reduction of CNA into the less toxic cinnamic alcohol. This discovery highlights the pivotal role of AmgRS in mediating defense against aldehyde biocides, emphasizing its significance in the persistence of P. aeruginosa, a pathogen associated with hospital-acquired infections and cystic fibrosis, and underscores the potential impact on clinical treatment strategies.https://journals.asm.org/doi/10.1128/spectrum.01699-24Pseudomonas aeruginosacinnamaldehydetwo-component regulatory systemsefflux pumpsantibiotic resistancecross resistance antibiotics essential oils |
| spellingShingle | Eline Dubois Vladimir Spasovski Patrick Plésiat Catherine Llanes Role of the two-component system AmgRS in early resistance of Pseudomonas aeruginosa to cinnamaldehyde Microbiology Spectrum Pseudomonas aeruginosa cinnamaldehyde two-component regulatory systems efflux pumps antibiotic resistance cross resistance antibiotics essential oils |
| title | Role of the two-component system AmgRS in early resistance of Pseudomonas aeruginosa to cinnamaldehyde |
| title_full | Role of the two-component system AmgRS in early resistance of Pseudomonas aeruginosa to cinnamaldehyde |
| title_fullStr | Role of the two-component system AmgRS in early resistance of Pseudomonas aeruginosa to cinnamaldehyde |
| title_full_unstemmed | Role of the two-component system AmgRS in early resistance of Pseudomonas aeruginosa to cinnamaldehyde |
| title_short | Role of the two-component system AmgRS in early resistance of Pseudomonas aeruginosa to cinnamaldehyde |
| title_sort | role of the two component system amgrs in early resistance of pseudomonas aeruginosa to cinnamaldehyde |
| topic | Pseudomonas aeruginosa cinnamaldehyde two-component regulatory systems efflux pumps antibiotic resistance cross resistance antibiotics essential oils |
| url | https://journals.asm.org/doi/10.1128/spectrum.01699-24 |
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