Fast and Simple Protocol for N-Glycome Analysis of Human Blood Plasma Proteome
N-glycome analysis of individual proteins and tissues is crucial for fundamental and applied biomedical research and medical diagnosis and plays an important role in the evaluation of the quality of biopharmaceutical and biotechnological products. The interest in this research area continues to grow...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/14/12/1551 |
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| author | Denis E. Maslov Anna N. Timoshchuk Alexander A. Bondar Maxim P. Golubev Anna G. Soplenkova Maja Hanic Sodbo Z. Sharapov Olga N. Leonova Yurii S. Aulchenko Tatiana S. Golubeva |
| author_facet | Denis E. Maslov Anna N. Timoshchuk Alexander A. Bondar Maxim P. Golubev Anna G. Soplenkova Maja Hanic Sodbo Z. Sharapov Olga N. Leonova Yurii S. Aulchenko Tatiana S. Golubeva |
| author_sort | Denis E. Maslov |
| collection | DOAJ |
| description | N-glycome analysis of individual proteins and tissues is crucial for fundamental and applied biomedical research and medical diagnosis and plays an important role in the evaluation of the quality of biopharmaceutical and biotechnological products. The interest in this research area continues to grow annually, thereby increasing the demand for the high-throughput profiling of human blood plasma N-glycome. In response to this need, we have developed an optimized, simple, and rapid protocol for the N-glycome profiling of human plasma proteins. This protocol encompasses the entire analysis cycle, from plasma isolation to N-glycan spectrum quantification. While the proposed method may have lower efficiency compared to already published high-throughput methods, its adaptability makes it suitable for implementation in virtually any molecular biological laboratory. |
| format | Article |
| id | doaj-art-0cd3a56b143c40cc88f3b3f145b01bc4 |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-0cd3a56b143c40cc88f3b3f145b01bc42024-12-27T14:13:46ZengMDPI AGBiomolecules2218-273X2024-12-011412155110.3390/biom14121551Fast and Simple Protocol for N-Glycome Analysis of Human Blood Plasma ProteomeDenis E. Maslov0Anna N. Timoshchuk1Alexander A. Bondar2Maxim P. Golubev3Anna G. Soplenkova4Maja Hanic5Sodbo Z. Sharapov6Olga N. Leonova7Yurii S. Aulchenko8Tatiana S. Golubeva9MSU Institute for Artificial Intelligence, Lomonosov Moscow State University, Moscow 119192, RussiaMSU Institute for Artificial Intelligence, Lomonosov Moscow State University, Moscow 119192, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk 630090, RussiaMSU Institute for Artificial Intelligence, Lomonosov Moscow State University, Moscow 119192, RussiaMSU Institute for Artificial Intelligence, Lomonosov Moscow State University, Moscow 119192, RussiaGenos Glycoscience Research Laboratory, 10000 Zagreb, CroatiaMSU Institute for Artificial Intelligence, Lomonosov Moscow State University, Moscow 119192, RussiaPriorov Central Institute of Traumatology and Orthopedic, Moscow 127299, RussiaMSU Institute for Artificial Intelligence, Lomonosov Moscow State University, Moscow 119192, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk 630090, RussiaN-glycome analysis of individual proteins and tissues is crucial for fundamental and applied biomedical research and medical diagnosis and plays an important role in the evaluation of the quality of biopharmaceutical and biotechnological products. The interest in this research area continues to grow annually, thereby increasing the demand for the high-throughput profiling of human blood plasma N-glycome. In response to this need, we have developed an optimized, simple, and rapid protocol for the N-glycome profiling of human plasma proteins. This protocol encompasses the entire analysis cycle, from plasma isolation to N-glycan spectrum quantification. While the proposed method may have lower efficiency compared to already published high-throughput methods, its adaptability makes it suitable for implementation in virtually any molecular biological laboratory.https://www.mdpi.com/2218-273X/14/12/1551N-glycosylationN-glycansxCGE-LIF analysishuman blood plasmapost-translational modificationsAPTS labeling |
| spellingShingle | Denis E. Maslov Anna N. Timoshchuk Alexander A. Bondar Maxim P. Golubev Anna G. Soplenkova Maja Hanic Sodbo Z. Sharapov Olga N. Leonova Yurii S. Aulchenko Tatiana S. Golubeva Fast and Simple Protocol for N-Glycome Analysis of Human Blood Plasma Proteome Biomolecules N-glycosylation N-glycans xCGE-LIF analysis human blood plasma post-translational modifications APTS labeling |
| title | Fast and Simple Protocol for N-Glycome Analysis of Human Blood Plasma Proteome |
| title_full | Fast and Simple Protocol for N-Glycome Analysis of Human Blood Plasma Proteome |
| title_fullStr | Fast and Simple Protocol for N-Glycome Analysis of Human Blood Plasma Proteome |
| title_full_unstemmed | Fast and Simple Protocol for N-Glycome Analysis of Human Blood Plasma Proteome |
| title_short | Fast and Simple Protocol for N-Glycome Analysis of Human Blood Plasma Proteome |
| title_sort | fast and simple protocol for n glycome analysis of human blood plasma proteome |
| topic | N-glycosylation N-glycans xCGE-LIF analysis human blood plasma post-translational modifications APTS labeling |
| url | https://www.mdpi.com/2218-273X/14/12/1551 |
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