NMR metabolomics as a complementary tool to brix-acid tests for navel orange quality control of long-term cold storage
Abstract Quality control plays a crucial role in maintaining the reputation of agricultural organizations by ensuring that their products meet the expected standards and preventing any loss during the packaging process. A significant responsibility of quality control is conducting periodic product a...
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Nature Portfolio
2024-12-01
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| Online Access: | https://doi.org/10.1038/s41598-024-77871-z |
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| author | Keeton H. Montgomery Aya Elhabashy Maria Del Carmen Reynoso Rivas Gurreet Brar V. V. Krishnan |
| author_facet | Keeton H. Montgomery Aya Elhabashy Maria Del Carmen Reynoso Rivas Gurreet Brar V. V. Krishnan |
| author_sort | Keeton H. Montgomery |
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| description | Abstract Quality control plays a crucial role in maintaining the reputation of agricultural organizations by ensuring that their products meet the expected standards and preventing any loss during the packaging process. A significant responsibility of quality control is conducting periodic product assessments. However, subjective interpretation during physical inspections of fruits can lead to variability in reporting. To counter this, assessing total soluble solids (Brix) and percent acidity (Acid) can provide a more objective approach. Nevertheless, it is essential to note that many fruit metabolites can impact these parameters. Nuclear magnetic resonance (NMR) spectroscopy, particularly 1H-NMR, has become a popular tool for quality control in recent years due to its precision, sample preservation, and high throughput analysis. This manuscript investigates if the standard Brix/Acid tests are directly related to the levels of metabolites during cold storage. Using citrus as the model system, a metabolomics analysis was conducted to identify patterns in the cold storage metabolite profiles of the juice, albedo, and flavedo tissues. The results show that Brix (or total dissolved solids) correlates well with sucrose, glucose, and fructose levels and moderately with choline levels. Acid (percent acidity) levels displayed a negative correlation with both fructose and choline levels. Interestingly, the formate levels were susceptible to storage time and directly related to Acid measurements. This study suggests metabolomics could be a complementary technique to quality control of fruits in cold storage, especially with cost-effective desktop NMR spectrometers. |
| format | Article |
| id | doaj-art-0cce8dd95a064d979008a161795b8db2 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-0cce8dd95a064d979008a161795b8db22024-12-08T12:24:49ZengNature PortfolioScientific Reports2045-23222024-12-0114111010.1038/s41598-024-77871-zNMR metabolomics as a complementary tool to brix-acid tests for navel orange quality control of long-term cold storageKeeton H. Montgomery0Aya Elhabashy1Maria Del Carmen Reynoso Rivas2Gurreet Brar3V. V. Krishnan4Department of Chemistry and Biochemistry, California State University FresnoDepartment of Chemistry and Biochemistry, California State University FresnoDepartment of Chemistry and Biochemistry, California State University FresnoDepartment of Plant Science, California State University FresnoDepartment of Chemistry and Biochemistry, California State University FresnoAbstract Quality control plays a crucial role in maintaining the reputation of agricultural organizations by ensuring that their products meet the expected standards and preventing any loss during the packaging process. A significant responsibility of quality control is conducting periodic product assessments. However, subjective interpretation during physical inspections of fruits can lead to variability in reporting. To counter this, assessing total soluble solids (Brix) and percent acidity (Acid) can provide a more objective approach. Nevertheless, it is essential to note that many fruit metabolites can impact these parameters. Nuclear magnetic resonance (NMR) spectroscopy, particularly 1H-NMR, has become a popular tool for quality control in recent years due to its precision, sample preservation, and high throughput analysis. This manuscript investigates if the standard Brix/Acid tests are directly related to the levels of metabolites during cold storage. Using citrus as the model system, a metabolomics analysis was conducted to identify patterns in the cold storage metabolite profiles of the juice, albedo, and flavedo tissues. The results show that Brix (or total dissolved solids) correlates well with sucrose, glucose, and fructose levels and moderately with choline levels. Acid (percent acidity) levels displayed a negative correlation with both fructose and choline levels. Interestingly, the formate levels were susceptible to storage time and directly related to Acid measurements. This study suggests metabolomics could be a complementary technique to quality control of fruits in cold storage, especially with cost-effective desktop NMR spectrometers.https://doi.org/10.1038/s41598-024-77871-zNuclear magnetic resonance (NMR)MetabolomicsBrix/Acid testsCold-storageCitrus |
| spellingShingle | Keeton H. Montgomery Aya Elhabashy Maria Del Carmen Reynoso Rivas Gurreet Brar V. V. Krishnan NMR metabolomics as a complementary tool to brix-acid tests for navel orange quality control of long-term cold storage Scientific Reports Nuclear magnetic resonance (NMR) Metabolomics Brix/Acid tests Cold-storage Citrus |
| title | NMR metabolomics as a complementary tool to brix-acid tests for navel orange quality control of long-term cold storage |
| title_full | NMR metabolomics as a complementary tool to brix-acid tests for navel orange quality control of long-term cold storage |
| title_fullStr | NMR metabolomics as a complementary tool to brix-acid tests for navel orange quality control of long-term cold storage |
| title_full_unstemmed | NMR metabolomics as a complementary tool to brix-acid tests for navel orange quality control of long-term cold storage |
| title_short | NMR metabolomics as a complementary tool to brix-acid tests for navel orange quality control of long-term cold storage |
| title_sort | nmr metabolomics as a complementary tool to brix acid tests for navel orange quality control of long term cold storage |
| topic | Nuclear magnetic resonance (NMR) Metabolomics Brix/Acid tests Cold-storage Citrus |
| url | https://doi.org/10.1038/s41598-024-77871-z |
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