Comparative Analysis of Infection Strategies of <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> and <i>P. syringae</i> pv. <i>tomato</i> in Different Host Plants
Plant pathogenic <i>Pseudomonas</i> species cause a variety of diseases in plants. Each <i>Pseudomonas</i> species employs different virulence factors and strategies for successful infection. Moreover, even the same bacterial pathogens can differentially utilize virulence fac...
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MDPI AG
2024-11-01
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| Series: | Bacteria |
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| Online Access: | https://www.mdpi.com/2674-1334/3/4/26 |
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| author | Nanami Sakata Giyu Usuki Kanon Yamamoto Yasuhiro Ishiga |
| author_facet | Nanami Sakata Giyu Usuki Kanon Yamamoto Yasuhiro Ishiga |
| author_sort | Nanami Sakata |
| collection | DOAJ |
| description | Plant pathogenic <i>Pseudomonas</i> species cause a variety of diseases in plants. Each <i>Pseudomonas</i> species employs different virulence factors and strategies for successful infection. Moreover, even the same bacterial pathogens can differentially utilize virulence factors against various host plants. However, there has been relatively less emphasis on comparing the infection strategies of a single bacterial pathogen on different hosts and different bacterial pathogens on a single host. Here, we investigated plant–pathogen interactions using two <i>Pseudomonas</i> species, <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> (<i>Pcal</i>) KB211 and <i>Pseudomonas syringae</i> pv. <i>tomato</i> (<i>Pst</i>) DC3000, and their host plants, cabbage and tomato. Our findings reveal distinct behaviors and virulence patterns across different host plants. <i>Pcal</i> multiplies to greater levels in cabbage compared to tomato, suggesting that <i>Pcal</i> is more adaptive in cabbage than tomato. Conversely, <i>Pst</i> showed robust multiplication in tomato even at lesser inoculum levels, indicating its aggressiveness in the apoplastic space. Gene expression analyses indicate that these pathogens utilize distinct virulence-related gene expression profiles depending on the host plant. These insights highlight the importance of revealing the spatiotemporal regulation mechanisms of virulence factors. |
| format | Article |
| id | doaj-art-0cbfbe62cba74038a9f8b83fcb138cd5 |
| institution | Kabale University |
| issn | 2674-1334 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Bacteria |
| spelling | doaj-art-0cbfbe62cba74038a9f8b83fcb138cd52024-12-27T14:10:33ZengMDPI AGBacteria2674-13342024-11-013437938910.3390/bacteria3040026Comparative Analysis of Infection Strategies of <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> and <i>P. syringae</i> pv. <i>tomato</i> in Different Host PlantsNanami Sakata0Giyu Usuki1Kanon Yamamoto2Yasuhiro Ishiga3Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8572, Ibaraki, JapanFaculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8572, Ibaraki, JapanFaculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8572, Ibaraki, JapanFaculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8572, Ibaraki, JapanPlant pathogenic <i>Pseudomonas</i> species cause a variety of diseases in plants. Each <i>Pseudomonas</i> species employs different virulence factors and strategies for successful infection. Moreover, even the same bacterial pathogens can differentially utilize virulence factors against various host plants. However, there has been relatively less emphasis on comparing the infection strategies of a single bacterial pathogen on different hosts and different bacterial pathogens on a single host. Here, we investigated plant–pathogen interactions using two <i>Pseudomonas</i> species, <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> (<i>Pcal</i>) KB211 and <i>Pseudomonas syringae</i> pv. <i>tomato</i> (<i>Pst</i>) DC3000, and their host plants, cabbage and tomato. Our findings reveal distinct behaviors and virulence patterns across different host plants. <i>Pcal</i> multiplies to greater levels in cabbage compared to tomato, suggesting that <i>Pcal</i> is more adaptive in cabbage than tomato. Conversely, <i>Pst</i> showed robust multiplication in tomato even at lesser inoculum levels, indicating its aggressiveness in the apoplastic space. Gene expression analyses indicate that these pathogens utilize distinct virulence-related gene expression profiles depending on the host plant. These insights highlight the importance of revealing the spatiotemporal regulation mechanisms of virulence factors.https://www.mdpi.com/2674-1334/3/4/26plant–pathogen interactions<i>Pseudomonas cannabina</i> pv. <i>alisalensis</i><i>Pseudomonas syringae</i> pv. <i>tomato</i>cabbagetomato |
| spellingShingle | Nanami Sakata Giyu Usuki Kanon Yamamoto Yasuhiro Ishiga Comparative Analysis of Infection Strategies of <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> and <i>P. syringae</i> pv. <i>tomato</i> in Different Host Plants Bacteria plant–pathogen interactions <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> <i>Pseudomonas syringae</i> pv. <i>tomato</i> cabbage tomato |
| title | Comparative Analysis of Infection Strategies of <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> and <i>P. syringae</i> pv. <i>tomato</i> in Different Host Plants |
| title_full | Comparative Analysis of Infection Strategies of <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> and <i>P. syringae</i> pv. <i>tomato</i> in Different Host Plants |
| title_fullStr | Comparative Analysis of Infection Strategies of <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> and <i>P. syringae</i> pv. <i>tomato</i> in Different Host Plants |
| title_full_unstemmed | Comparative Analysis of Infection Strategies of <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> and <i>P. syringae</i> pv. <i>tomato</i> in Different Host Plants |
| title_short | Comparative Analysis of Infection Strategies of <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> and <i>P. syringae</i> pv. <i>tomato</i> in Different Host Plants |
| title_sort | comparative analysis of infection strategies of i pseudomonas cannabina i pv i alisalensis i and i p syringae i pv i tomato i in different host plants |
| topic | plant–pathogen interactions <i>Pseudomonas cannabina</i> pv. <i>alisalensis</i> <i>Pseudomonas syringae</i> pv. <i>tomato</i> cabbage tomato |
| url | https://www.mdpi.com/2674-1334/3/4/26 |
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