Development of a simple parametric approach to correct fluorescence signal for reabsorption and its implications for GPP estimates: a case study for alfalfa crop

Solar-induced chlorophyll Fluorescence (F) retrievals at canopy level remain challenging due to canopy reabsorption and scattering that affect the F’ spectra at the top of canopy (TOC). This study presents FTOC retrievals over an alfalfa crop in Italy, based on field spectroscopy data collected from...

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Main Authors: Luis Miguel da Costa, Sergio Cogliati, Ilaria Cesana, Tommaso Julitta, Marco Celesti, Marin Tudoroiu, Lorenzo Genesio, Franco Miglietta, Newton La Scala, Roberto Colombo
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:European Journal of Remote Sensing
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Online Access:https://www.tandfonline.com/doi/10.1080/22797254.2025.2540962
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author Luis Miguel da Costa
Sergio Cogliati
Ilaria Cesana
Tommaso Julitta
Marco Celesti
Marin Tudoroiu
Lorenzo Genesio
Franco Miglietta
Newton La Scala
Roberto Colombo
author_facet Luis Miguel da Costa
Sergio Cogliati
Ilaria Cesana
Tommaso Julitta
Marco Celesti
Marin Tudoroiu
Lorenzo Genesio
Franco Miglietta
Newton La Scala
Roberto Colombo
author_sort Luis Miguel da Costa
collection DOAJ
description Solar-induced chlorophyll Fluorescence (F) retrievals at canopy level remain challenging due to canopy reabsorption and scattering that affect the F’ spectra at the top of canopy (TOC). This study presents FTOC retrievals over an alfalfa crop in Italy, based on field spectroscopy data collected from May to July 2018 A novel, simple parametric correction for reabsorption, based on radiative transfer model, was applied to obtain a corrected spectrum (FRC). We also investigated the relationships of FTOC and FRC spectra with Gross Primary Production (GPP) estimated from CO2 fluxes measured through Eddy Covariance. Fluorescence at selected wavelengths was analyzed and combined to estimate GPP using linear, stepwise, and Random Forest approaches. The Fluorescence yield (Fy) and Light Use Efficiency (LUE) relationship was also investigated. The correlation between GPP and FTOC peaked on the red-edge and far-red spectral regions, furthermore, combined wavelengths improved GPP estimates around 10%. Meanwhile, GPP – FRC correlation was constant across spectra. Fy explained 90% of LUE variability. Overall, our results indicate that FTOC was efficiently correct for reabsorption and that using full FTOC spectrum enhances GPP estimation in alfalfa and can be seen as a precursor for a better characterization of the link F-photosynthesis for other crops..
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spelling doaj-art-0ca6db37f3d44ad8b08c4dab4e5cb12f2025-08-20T03:56:17ZengTaylor & Francis GroupEuropean Journal of Remote Sensing2279-72542025-12-0158110.1080/22797254.2025.2540962Development of a simple parametric approach to correct fluorescence signal for reabsorption and its implications for GPP estimates: a case study for alfalfa cropLuis Miguel da Costa0Sergio Cogliati1Ilaria Cesana2Tommaso Julitta3Marco Celesti4Marin Tudoroiu5Lorenzo Genesio6Franco Miglietta7Newton La Scala8Roberto Colombo9Department of Engineering and Exact Sciences, São Paulo State University Faculty of Agrarian and Veterinary Sciences (FCAV–UNESP), Jaboticabal, BrazilRemote Sensing of Environmental Dynamics Laborator DISAT, University of Milano-Bicocca, Milano, ItalyRemote Sensing of Environmental Dynamics Laborator DISAT, University of Milano-Bicocca, Milano, ItalyJB-Hyperspectral Devices GmbH, Düsseldorf, GermanyHE Space for ESA – European Space Agency, ESTEC, Noordwijk, the NetherlandsEuropean Space Agency, ESRIN, Frascati (Rome), ItalyInstitute of Bioeconomy – IBE, National Research Council - CNR, Florence, ItalyInstitute of Bioeconomy – IBE, National Research Council - CNR, Florence, ItalyDepartment of Engineering and Exact Sciences, São Paulo State University Faculty of Agrarian and Veterinary Sciences (FCAV–UNESP), Jaboticabal, BrazilRemote Sensing of Environmental Dynamics Laborator DISAT, University of Milano-Bicocca, Milano, ItalySolar-induced chlorophyll Fluorescence (F) retrievals at canopy level remain challenging due to canopy reabsorption and scattering that affect the F’ spectra at the top of canopy (TOC). This study presents FTOC retrievals over an alfalfa crop in Italy, based on field spectroscopy data collected from May to July 2018 A novel, simple parametric correction for reabsorption, based on radiative transfer model, was applied to obtain a corrected spectrum (FRC). We also investigated the relationships of FTOC and FRC spectra with Gross Primary Production (GPP) estimated from CO2 fluxes measured through Eddy Covariance. Fluorescence at selected wavelengths was analyzed and combined to estimate GPP using linear, stepwise, and Random Forest approaches. The Fluorescence yield (Fy) and Light Use Efficiency (LUE) relationship was also investigated. The correlation between GPP and FTOC peaked on the red-edge and far-red spectral regions, furthermore, combined wavelengths improved GPP estimates around 10%. Meanwhile, GPP – FRC correlation was constant across spectra. Fy explained 90% of LUE variability. Overall, our results indicate that FTOC was efficiently correct for reabsorption and that using full FTOC spectrum enhances GPP estimation in alfalfa and can be seen as a precursor for a better characterization of the link F-photosynthesis for other crops..https://www.tandfonline.com/doi/10.1080/22797254.2025.2540962Solar-induced chlorophyll fluorescencereabsorption correctiongross primary productionlight use efficiencyremote sensing
spellingShingle Luis Miguel da Costa
Sergio Cogliati
Ilaria Cesana
Tommaso Julitta
Marco Celesti
Marin Tudoroiu
Lorenzo Genesio
Franco Miglietta
Newton La Scala
Roberto Colombo
Development of a simple parametric approach to correct fluorescence signal for reabsorption and its implications for GPP estimates: a case study for alfalfa crop
European Journal of Remote Sensing
Solar-induced chlorophyll fluorescence
reabsorption correction
gross primary production
light use efficiency
remote sensing
title Development of a simple parametric approach to correct fluorescence signal for reabsorption and its implications for GPP estimates: a case study for alfalfa crop
title_full Development of a simple parametric approach to correct fluorescence signal for reabsorption and its implications for GPP estimates: a case study for alfalfa crop
title_fullStr Development of a simple parametric approach to correct fluorescence signal for reabsorption and its implications for GPP estimates: a case study for alfalfa crop
title_full_unstemmed Development of a simple parametric approach to correct fluorescence signal for reabsorption and its implications for GPP estimates: a case study for alfalfa crop
title_short Development of a simple parametric approach to correct fluorescence signal for reabsorption and its implications for GPP estimates: a case study for alfalfa crop
title_sort development of a simple parametric approach to correct fluorescence signal for reabsorption and its implications for gpp estimates a case study for alfalfa crop
topic Solar-induced chlorophyll fluorescence
reabsorption correction
gross primary production
light use efficiency
remote sensing
url https://www.tandfonline.com/doi/10.1080/22797254.2025.2540962
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