Anti-influenza effects of Aloe vera gel on MDCK cells: protective efficacy against ultraviolet-C exposure and analysis of biochemical characteristics and microbial activities
The main objective of the current study was to examine the anti-influenza effects of Aloe vera gel on the Madin–Darby Canine Kidney (MDCK) cell line, as well as its protective efficacy against ultraviolet-C exposure, by studying its biochemical characterization and microbial activities. The lowest I...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Sustainable Food Systems |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsufs.2024.1508809/full |
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| author | Rasha A. Al-Eisa Rokayya Sami Ruqaiah I. Bedaiwi Roqayah H. Kadi Suzan A. Abushal Doaa Mahmoud Johari Afnan M. Alnajeebi Reham M. Algheshairy Abeer A. Abu-Zaid Sami A. Al-Dhumri |
| author_facet | Rasha A. Al-Eisa Rokayya Sami Ruqaiah I. Bedaiwi Roqayah H. Kadi Suzan A. Abushal Doaa Mahmoud Johari Afnan M. Alnajeebi Reham M. Algheshairy Abeer A. Abu-Zaid Sami A. Al-Dhumri |
| author_sort | Rasha A. Al-Eisa |
| collection | DOAJ |
| description | The main objective of the current study was to examine the anti-influenza effects of Aloe vera gel on the Madin–Darby Canine Kidney (MDCK) cell line, as well as its protective efficacy against ultraviolet-C exposure, by studying its biochemical characterization and microbial activities. The lowest IC50 value (4.23 mg/mL) was reported for the treated cells, with a concentration of 8 mg/ML of Aloe vera extract after contamination with the AP/R/8 virus. After 2 h of ultraviolet-C exposure, all cells in the control group and those with single-sided gel application died, while the cells treated with the gel on both sides showed 29.000 viable cells. The lowest inhibition activity was detected against Bacillus subtilis (2.20 mm) at a concentration of 20 μL. In comparison, the highest inhibition activity was observed against Pseudomonas aeruginosa (21.29 mm) at a concentration of 100 μL. The highest values were detected in the Aloe vera gel samples in the following sequence: magnesium (28.43 mg/100 g), phosphorus (2.98 mg/100 g), zinc (143.01 μg/100 g), selenium (137.18 μg/100 g), iron (19.78 μg/100 g), chromium (15.40 μg/100 g), aluminum (12.35 μg/100 g), and vanadium (8.70 μg/100 g). On the other hand, trace elements such as cadmium, cobalt, and nickel showed the lowest concentrations (0.05, 0.12, and 0.23 μg/100 g, respectively). The plant contained high quantities of phenols, flavonoids, tannins, saponins, and anthraquinones, along with high antioxidant activity (1,1-diphenyl-2picrylhydrazyl, DPPH) and ascorbic acid content. Based on these observations, it can be concluded that Aloe vera plants contain variable compounds that may be responsible for their therapeutic and pharmacological uses. |
| format | Article |
| id | doaj-art-0b994da1ffc4461e96e2cc92c7bebba8 |
| institution | Kabale University |
| issn | 2571-581X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Sustainable Food Systems |
| spelling | doaj-art-0b994da1ffc4461e96e2cc92c7bebba82025-01-06T12:49:25ZengFrontiers Media S.A.Frontiers in Sustainable Food Systems2571-581X2025-01-01810.3389/fsufs.2024.15088091508809Anti-influenza effects of Aloe vera gel on MDCK cells: protective efficacy against ultraviolet-C exposure and analysis of biochemical characteristics and microbial activitiesRasha A. Al-Eisa0Rokayya Sami1Ruqaiah I. Bedaiwi2Roqayah H. Kadi3Suzan A. Abushal4Doaa Mahmoud Johari5Afnan M. Alnajeebi6Reham M. Algheshairy7Abeer A. Abu-Zaid8Sami A. Al-Dhumri9Department of Biology, College of Sciences, Taif University, Taif, Saudi ArabiaDepartment of Food Science and Nutrition, College of Sciences, Taif University, Taif, Saudi ArabiaDepartment of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, Saudi ArabiaDepartment of Biological Sciences, College of Science, University of Jeddah, Jeddah, Saudi ArabiaProgram of Food Sciences and Nutrition, Turabah University College, Taif University, Taif, Saudi ArabiaPharmacy Administration Department, Taif Health Cluster, Ministry of Health, Taif, Saudi ArabiaDepartment of Biochemistry, College of Science, University of Jeddah, Jeddah, Saudi ArabiaDepartment of Food Science and Human Nutrition, College of Agriculture and Food, Qassim University, Buraydah, Saudi ArabiaDepartment of Food Science and Nutrition, Alkhurmah University College, Taif University, Taif, Saudi Arabia0Department of Biology, Al Khurma University College, Taif University, Taif, Saudi ArabiaThe main objective of the current study was to examine the anti-influenza effects of Aloe vera gel on the Madin–Darby Canine Kidney (MDCK) cell line, as well as its protective efficacy against ultraviolet-C exposure, by studying its biochemical characterization and microbial activities. The lowest IC50 value (4.23 mg/mL) was reported for the treated cells, with a concentration of 8 mg/ML of Aloe vera extract after contamination with the AP/R/8 virus. After 2 h of ultraviolet-C exposure, all cells in the control group and those with single-sided gel application died, while the cells treated with the gel on both sides showed 29.000 viable cells. The lowest inhibition activity was detected against Bacillus subtilis (2.20 mm) at a concentration of 20 μL. In comparison, the highest inhibition activity was observed against Pseudomonas aeruginosa (21.29 mm) at a concentration of 100 μL. The highest values were detected in the Aloe vera gel samples in the following sequence: magnesium (28.43 mg/100 g), phosphorus (2.98 mg/100 g), zinc (143.01 μg/100 g), selenium (137.18 μg/100 g), iron (19.78 μg/100 g), chromium (15.40 μg/100 g), aluminum (12.35 μg/100 g), and vanadium (8.70 μg/100 g). On the other hand, trace elements such as cadmium, cobalt, and nickel showed the lowest concentrations (0.05, 0.12, and 0.23 μg/100 g, respectively). The plant contained high quantities of phenols, flavonoids, tannins, saponins, and anthraquinones, along with high antioxidant activity (1,1-diphenyl-2picrylhydrazyl, DPPH) and ascorbic acid content. Based on these observations, it can be concluded that Aloe vera plants contain variable compounds that may be responsible for their therapeutic and pharmacological uses.https://www.frontiersin.org/articles/10.3389/fsufs.2024.1508809/fullAloe vera gelanti-influenzaultraviolet-Cmicrobial activitiesnutrientsphytochemical analysis |
| spellingShingle | Rasha A. Al-Eisa Rokayya Sami Ruqaiah I. Bedaiwi Roqayah H. Kadi Suzan A. Abushal Doaa Mahmoud Johari Afnan M. Alnajeebi Reham M. Algheshairy Abeer A. Abu-Zaid Sami A. Al-Dhumri Anti-influenza effects of Aloe vera gel on MDCK cells: protective efficacy against ultraviolet-C exposure and analysis of biochemical characteristics and microbial activities Frontiers in Sustainable Food Systems Aloe vera gel anti-influenza ultraviolet-C microbial activities nutrients phytochemical analysis |
| title | Anti-influenza effects of Aloe vera gel on MDCK cells: protective efficacy against ultraviolet-C exposure and analysis of biochemical characteristics and microbial activities |
| title_full | Anti-influenza effects of Aloe vera gel on MDCK cells: protective efficacy against ultraviolet-C exposure and analysis of biochemical characteristics and microbial activities |
| title_fullStr | Anti-influenza effects of Aloe vera gel on MDCK cells: protective efficacy against ultraviolet-C exposure and analysis of biochemical characteristics and microbial activities |
| title_full_unstemmed | Anti-influenza effects of Aloe vera gel on MDCK cells: protective efficacy against ultraviolet-C exposure and analysis of biochemical characteristics and microbial activities |
| title_short | Anti-influenza effects of Aloe vera gel on MDCK cells: protective efficacy against ultraviolet-C exposure and analysis of biochemical characteristics and microbial activities |
| title_sort | anti influenza effects of aloe vera gel on mdck cells protective efficacy against ultraviolet c exposure and analysis of biochemical characteristics and microbial activities |
| topic | Aloe vera gel anti-influenza ultraviolet-C microbial activities nutrients phytochemical analysis |
| url | https://www.frontiersin.org/articles/10.3389/fsufs.2024.1508809/full |
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