Evaluation of a commercial immunochromatographic strip assay (ICT) for rapid detection of bovine, porcine and human Rotavirus A
The most frequent diarrhoeal pathogen “Rotavirus” is commonly detected by Ribo Nucleic Acid- Polyacrylamide Gel Electrophoresis (RNA-PAGE), Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Immunoassay (Enzyme Linked Immunosorbent Assay) in humans. However, use of these tests is limited i...
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Elsevier
2024-12-01
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| Series: | The Microbe |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950194624001493 |
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| author | Bilal Ahmad Malla Zunjar Baburao Dubal Shubham Saini Obli Rajendran VinodhKumar Aquil Mohmad P.T. Waseem Mirsab Mufeeda Beegum Vibha Singh Kiran Narayan Bhilegaonkar |
| author_facet | Bilal Ahmad Malla Zunjar Baburao Dubal Shubham Saini Obli Rajendran VinodhKumar Aquil Mohmad P.T. Waseem Mirsab Mufeeda Beegum Vibha Singh Kiran Narayan Bhilegaonkar |
| author_sort | Bilal Ahmad Malla |
| collection | DOAJ |
| description | The most frequent diarrhoeal pathogen “Rotavirus” is commonly detected by Ribo Nucleic Acid- Polyacrylamide Gel Electrophoresis (RNA-PAGE), Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Immunoassay (Enzyme Linked Immunosorbent Assay) in humans. However, use of these tests is limited in animals therefore, penside test like Immunochromatographic test/Lateral Flow Assay (ICT/LFA) is necessary. The present study was carried out to compare the diagnostic efficacy of rapid commercial ICT strip assay, RNA-PAGE and RT-PCR for specific detection of Rotavirus A (RVA) from stool samples of calves, piglets and children. A total of 313 faecal samples were collected between November 2022 to April 2023 from children below 5years of age (n = 100), calves (n = 100) and piglets (n = 113) ≤ 3 months of age and were screened by RNA-PAGE, RT-PCR and commercial ICT kit for rotavirus A.The overall positivity of RVA from human, calves and piglets using RNA-PAGE, RT-PCR and LFA was found to be 35 % (35/100), 8 % (8/100) and 14.16 % (16/113), respectively. Higher positivity of rotavirus was recorded in male children (44.26 %, 27/61) than in female children (20.51 %, 8/39). The kappa agreement between LFA and RT-PCR was 0.79 (substantial agreement); between LFA and RNA-PAGE was 0.61 (substantially low agreement) and between RNA-PAGE and RT-PCR was found to be 0.75 (substantial agreement). Relative diagnostic sensitivity and specificity of LFA in comparison with RT-PCR was 74.54 % and 98.44 %, respectively and in comparison with RNA-PAGE was 72.97 % and 93.47 %, respectively. RNA-PAGE in comparison with RT-PCR is having diagnostic sensitivity and specificity of 65.45 % and 99.6 %, respectively. In conclusion, the ICT strip assay is a rapid, convenient and effective method with satisfactory efficacy for detection of RVA from children, calves and piglets. ICT fulfilled the WHO ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and robust, Equipment-free and Delivered) criteria for point-of-care testing. It can be useful in determining rotavirus outbreaks in resource-limited settings. |
| format | Article |
| id | doaj-art-0b6ebc70bc3b4d0f8f78bed633138b84 |
| institution | Kabale University |
| issn | 2950-1946 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | The Microbe |
| spelling | doaj-art-0b6ebc70bc3b4d0f8f78bed633138b842024-12-18T08:55:43ZengElsevierThe Microbe2950-19462024-12-015100182Evaluation of a commercial immunochromatographic strip assay (ICT) for rapid detection of bovine, porcine and human Rotavirus ABilal Ahmad Malla0Zunjar Baburao Dubal1Shubham Saini2Obli Rajendran VinodhKumar3Aquil Mohmad4P.T. Waseem Mirsab5Mufeeda Beegum6Vibha Singh7Kiran Narayan Bhilegaonkar8Division of Veterinary Public Health, ICAR, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India; Department of Veterinary Technology, Higher Education Department, Government of Jammu and Kashmir, India; Correspondence to: Veterinary Technology, Higher Education Department, J & K, India.ICAR-NIVEDI, Yelahanka, Bengaluru 560064, India; Corresponding author.Division of Veterinary Public Health, ICAR, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, IndiaDivision of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar 243122, IndiaDepartment of Veterinary Technology, Higher Education Department, Government of Jammu and Kashmir, IndiaDivision of Veterinary Public Health, ICAR, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, IndiaDivision of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar 243122, IndiaDivision of Veterinary Public Health, ICAR, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, IndiaDivision of Veterinary Public Health, ICAR, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, IndiaThe most frequent diarrhoeal pathogen “Rotavirus” is commonly detected by Ribo Nucleic Acid- Polyacrylamide Gel Electrophoresis (RNA-PAGE), Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Immunoassay (Enzyme Linked Immunosorbent Assay) in humans. However, use of these tests is limited in animals therefore, penside test like Immunochromatographic test/Lateral Flow Assay (ICT/LFA) is necessary. The present study was carried out to compare the diagnostic efficacy of rapid commercial ICT strip assay, RNA-PAGE and RT-PCR for specific detection of Rotavirus A (RVA) from stool samples of calves, piglets and children. A total of 313 faecal samples were collected between November 2022 to April 2023 from children below 5years of age (n = 100), calves (n = 100) and piglets (n = 113) ≤ 3 months of age and were screened by RNA-PAGE, RT-PCR and commercial ICT kit for rotavirus A.The overall positivity of RVA from human, calves and piglets using RNA-PAGE, RT-PCR and LFA was found to be 35 % (35/100), 8 % (8/100) and 14.16 % (16/113), respectively. Higher positivity of rotavirus was recorded in male children (44.26 %, 27/61) than in female children (20.51 %, 8/39). The kappa agreement between LFA and RT-PCR was 0.79 (substantial agreement); between LFA and RNA-PAGE was 0.61 (substantially low agreement) and between RNA-PAGE and RT-PCR was found to be 0.75 (substantial agreement). Relative diagnostic sensitivity and specificity of LFA in comparison with RT-PCR was 74.54 % and 98.44 %, respectively and in comparison with RNA-PAGE was 72.97 % and 93.47 %, respectively. RNA-PAGE in comparison with RT-PCR is having diagnostic sensitivity and specificity of 65.45 % and 99.6 %, respectively. In conclusion, the ICT strip assay is a rapid, convenient and effective method with satisfactory efficacy for detection of RVA from children, calves and piglets. ICT fulfilled the WHO ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and robust, Equipment-free and Delivered) criteria for point-of-care testing. It can be useful in determining rotavirus outbreaks in resource-limited settings.http://www.sciencedirect.com/science/article/pii/S2950194624001493FaecesCalvesChildrenPigletsRVAICT/LFA |
| spellingShingle | Bilal Ahmad Malla Zunjar Baburao Dubal Shubham Saini Obli Rajendran VinodhKumar Aquil Mohmad P.T. Waseem Mirsab Mufeeda Beegum Vibha Singh Kiran Narayan Bhilegaonkar Evaluation of a commercial immunochromatographic strip assay (ICT) for rapid detection of bovine, porcine and human Rotavirus A The Microbe Faeces Calves Children Piglets RVA ICT/LFA |
| title | Evaluation of a commercial immunochromatographic strip assay (ICT) for rapid detection of bovine, porcine and human Rotavirus A |
| title_full | Evaluation of a commercial immunochromatographic strip assay (ICT) for rapid detection of bovine, porcine and human Rotavirus A |
| title_fullStr | Evaluation of a commercial immunochromatographic strip assay (ICT) for rapid detection of bovine, porcine and human Rotavirus A |
| title_full_unstemmed | Evaluation of a commercial immunochromatographic strip assay (ICT) for rapid detection of bovine, porcine and human Rotavirus A |
| title_short | Evaluation of a commercial immunochromatographic strip assay (ICT) for rapid detection of bovine, porcine and human Rotavirus A |
| title_sort | evaluation of a commercial immunochromatographic strip assay ict for rapid detection of bovine porcine and human rotavirus a |
| topic | Faeces Calves Children Piglets RVA ICT/LFA |
| url | http://www.sciencedirect.com/science/article/pii/S2950194624001493 |
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