Identification of 2,4-Di-<i>tert</i>-Butylphenol as an Antimicrobial Agent Against <i>Cutibacterium acnes</i> Bacteria from Rwandan Propolis
Background/Objectives: Acne is the most prevalent dermatological condition among humans, affecting approximately 80% of adolescents during puberty. To date, numerous compounds have been used for acne treatment, including erythromycin ointments and antiseptics, with varying degrees of success. The em...
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2024-11-01
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| Series: | Antibiotics |
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| author | Florent Rouvier Lydia Abou Emmanuel Wafo Perrine Andre Julien Cheyrol Mohamed-Mohsen Khacef Claude Nappez Hubert Lepidi Jean Michel Brunel |
| author_facet | Florent Rouvier Lydia Abou Emmanuel Wafo Perrine Andre Julien Cheyrol Mohamed-Mohsen Khacef Claude Nappez Hubert Lepidi Jean Michel Brunel |
| author_sort | Florent Rouvier |
| collection | DOAJ |
| description | Background/Objectives: Acne is the most prevalent dermatological condition among humans, affecting approximately 80% of adolescents during puberty. To date, numerous compounds have been used for acne treatment, including erythromycin ointments and antiseptics, with varying degrees of success. The emergence of erythromycin-resistant <i>C. acnes</i> strains has spurred the search for new antimicrobial agents, particularly from natural sources. Methods: Propolis collected in Rwanda was extracted and fractionated by flash chromatography and tested against <i>C. acnes</i> growth by using NCLSI recommendations. Results: In our research, we identified a molecule, 2,4-Di-<i>tert</i>-butylphenol (2,4-DTBP) which inhivbited the <i>C. acnes</i> growth at a concentration of 16 µg/mL. Based on these results, we formulated an ointment (1%) using OFAP18 and petroleum jelly for the potential treatment of acne using a mouse model. Conclusions: In vitro and in vivo evidence suggests that 2,4-DTBP has anti-inflammatory properties and could effectively manage the overgrowth of <i>C. acnes</i> as well as serve as a potent alternative for the formulation of an active propolis ointment for acne treatment. |
| format | Article |
| id | doaj-art-0b5c9d0e250f48068eb0b8ea2a8de1cc |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-0b5c9d0e250f48068eb0b8ea2a8de1cc2024-11-26T17:46:39ZengMDPI AGAntibiotics2079-63822024-11-011311108010.3390/antibiotics13111080Identification of 2,4-Di-<i>tert</i>-Butylphenol as an Antimicrobial Agent Against <i>Cutibacterium acnes</i> Bacteria from Rwandan PropolisFlorent Rouvier0Lydia Abou1Emmanuel Wafo2Perrine Andre3Julien Cheyrol4Mohamed-Mohsen Khacef5Claude Nappez6Hubert Lepidi7Jean Michel Brunel8Aix Marseille Université, INSERM, SSA, MCT, 13385 Marseille, FranceC2VN, Aix Marseille Université, INSERM 1263, INRAE 1260, 13385 Marseille, FranceAix Marseille Université, INSERM, SSA, MCT, 13385 Marseille, FranceCEFOS, UAR2020, Aix Marseille Université, Campus Timone, 27 Boulevard Jean Moulin, 13005 Marseille, FranceCEFOS, UAR2020, Aix Marseille Université, Campus Timone, 27 Boulevard Jean Moulin, 13005 Marseille, FranceCEFOS, UAR2020, Aix Marseille Université, Campus Timone, 27 Boulevard Jean Moulin, 13005 Marseille, FranceCEFOS, UAR2020, Aix Marseille Université, Campus Timone, 27 Boulevard Jean Moulin, 13005 Marseille, FranceLaboratoire d’Anatomie Pathologique, Hôpital de la Timone, 13005 Marseille, FranceAix Marseille Université, INSERM, SSA, MCT, 13385 Marseille, FranceBackground/Objectives: Acne is the most prevalent dermatological condition among humans, affecting approximately 80% of adolescents during puberty. To date, numerous compounds have been used for acne treatment, including erythromycin ointments and antiseptics, with varying degrees of success. The emergence of erythromycin-resistant <i>C. acnes</i> strains has spurred the search for new antimicrobial agents, particularly from natural sources. Methods: Propolis collected in Rwanda was extracted and fractionated by flash chromatography and tested against <i>C. acnes</i> growth by using NCLSI recommendations. Results: In our research, we identified a molecule, 2,4-Di-<i>tert</i>-butylphenol (2,4-DTBP) which inhivbited the <i>C. acnes</i> growth at a concentration of 16 µg/mL. Based on these results, we formulated an ointment (1%) using OFAP18 and petroleum jelly for the potential treatment of acne using a mouse model. Conclusions: In vitro and in vivo evidence suggests that 2,4-DTBP has anti-inflammatory properties and could effectively manage the overgrowth of <i>C. acnes</i> as well as serve as a potent alternative for the formulation of an active propolis ointment for acne treatment.https://www.mdpi.com/2079-6382/13/11/1080acneantibacterial activity<i>Cutibacterium acnes</i>2,4-Di-<i>tert</i>-butylphenolointmentpropolis |
| spellingShingle | Florent Rouvier Lydia Abou Emmanuel Wafo Perrine Andre Julien Cheyrol Mohamed-Mohsen Khacef Claude Nappez Hubert Lepidi Jean Michel Brunel Identification of 2,4-Di-<i>tert</i>-Butylphenol as an Antimicrobial Agent Against <i>Cutibacterium acnes</i> Bacteria from Rwandan Propolis Antibiotics acne antibacterial activity <i>Cutibacterium acnes</i> 2,4-Di-<i>tert</i>-butylphenol ointment propolis |
| title | Identification of 2,4-Di-<i>tert</i>-Butylphenol as an Antimicrobial Agent Against <i>Cutibacterium acnes</i> Bacteria from Rwandan Propolis |
| title_full | Identification of 2,4-Di-<i>tert</i>-Butylphenol as an Antimicrobial Agent Against <i>Cutibacterium acnes</i> Bacteria from Rwandan Propolis |
| title_fullStr | Identification of 2,4-Di-<i>tert</i>-Butylphenol as an Antimicrobial Agent Against <i>Cutibacterium acnes</i> Bacteria from Rwandan Propolis |
| title_full_unstemmed | Identification of 2,4-Di-<i>tert</i>-Butylphenol as an Antimicrobial Agent Against <i>Cutibacterium acnes</i> Bacteria from Rwandan Propolis |
| title_short | Identification of 2,4-Di-<i>tert</i>-Butylphenol as an Antimicrobial Agent Against <i>Cutibacterium acnes</i> Bacteria from Rwandan Propolis |
| title_sort | identification of 2 4 di i tert i butylphenol as an antimicrobial agent against i cutibacterium acnes i bacteria from rwandan propolis |
| topic | acne antibacterial activity <i>Cutibacterium acnes</i> 2,4-Di-<i>tert</i>-butylphenol ointment propolis |
| url | https://www.mdpi.com/2079-6382/13/11/1080 |
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