A lysosome-targeted fluorescent probe with large Stokes shift for visualizing biothiols in vivo and in vitro
Summary: Lysosomal biothiols play critical roles in numerous cellular processes and diseases. Researching an effective method for real-time labeling biothiols in lysosomes is of great significance and urgency, as it could provide essential information for the diagnosis of relevant diseases. In this...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224025598 |
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| author | Taotao Zhao Tong Zhang Zijun Tao Zhe Zhou Xiaofeng Xia Zhengjun Wu Feiyi Wang Jun Ren Erfei Wang |
| author_facet | Taotao Zhao Tong Zhang Zijun Tao Zhe Zhou Xiaofeng Xia Zhengjun Wu Feiyi Wang Jun Ren Erfei Wang |
| author_sort | Taotao Zhao |
| collection | DOAJ |
| description | Summary: Lysosomal biothiols play critical roles in numerous cellular processes and diseases. Researching an effective method for real-time labeling biothiols in lysosomes is of great significance and urgency, as it could provide essential information for the diagnosis of relevant diseases. In this study, we developed a lysosome-targeted fluorescent probe (LY-DCM-P) with a large Stokes shift of 150 nm for the sensitive and selective detection of biothiols in vivo and in vitro. Additionally, LY-DCM-P showed low cytotoxicity and excellent lysosome-targeted ability. The probe was successfully employed to monitor fluctuations in lysosomal biothiols in various living systems, enabling enormous potential to accurately monitor the occurrence and progress of biothiol-related diseases. |
| format | Article |
| id | doaj-art-0b3d4f35053a4f96a70ed8b2bbce8f9e |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-0b3d4f35053a4f96a70ed8b2bbce8f9e2024-12-22T05:28:58ZengElsevieriScience2589-00422024-12-012712111334A lysosome-targeted fluorescent probe with large Stokes shift for visualizing biothiols in vivo and in vitroTaotao Zhao0Tong Zhang1Zijun Tao2Zhe Zhou3Xiaofeng Xia4Zhengjun Wu5Feiyi Wang6Jun Ren7Erfei Wang8Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, P.R. ChinaHubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, P.R. ChinaHubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, P.R. ChinaHubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, P.R. ChinaHubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, P.R. ChinaHubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, P.R. China; Corresponding authorHubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, P.R. ChinaHubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, P.R. China; Corresponding authorHubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, P.R. China; Corresponding authorSummary: Lysosomal biothiols play critical roles in numerous cellular processes and diseases. Researching an effective method for real-time labeling biothiols in lysosomes is of great significance and urgency, as it could provide essential information for the diagnosis of relevant diseases. In this study, we developed a lysosome-targeted fluorescent probe (LY-DCM-P) with a large Stokes shift of 150 nm for the sensitive and selective detection of biothiols in vivo and in vitro. Additionally, LY-DCM-P showed low cytotoxicity and excellent lysosome-targeted ability. The probe was successfully employed to monitor fluctuations in lysosomal biothiols in various living systems, enabling enormous potential to accurately monitor the occurrence and progress of biothiol-related diseases.http://www.sciencedirect.com/science/article/pii/S2589004224025598ChemistryPhysicsBiological sciences |
| spellingShingle | Taotao Zhao Tong Zhang Zijun Tao Zhe Zhou Xiaofeng Xia Zhengjun Wu Feiyi Wang Jun Ren Erfei Wang A lysosome-targeted fluorescent probe with large Stokes shift for visualizing biothiols in vivo and in vitro iScience Chemistry Physics Biological sciences |
| title | A lysosome-targeted fluorescent probe with large Stokes shift for visualizing biothiols in vivo and in vitro |
| title_full | A lysosome-targeted fluorescent probe with large Stokes shift for visualizing biothiols in vivo and in vitro |
| title_fullStr | A lysosome-targeted fluorescent probe with large Stokes shift for visualizing biothiols in vivo and in vitro |
| title_full_unstemmed | A lysosome-targeted fluorescent probe with large Stokes shift for visualizing biothiols in vivo and in vitro |
| title_short | A lysosome-targeted fluorescent probe with large Stokes shift for visualizing biothiols in vivo and in vitro |
| title_sort | lysosome targeted fluorescent probe with large stokes shift for visualizing biothiols in vivo and in vitro |
| topic | Chemistry Physics Biological sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224025598 |
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