Recent progress in prompt molecular detection of liquid biopsy using Cas enzymes: innovative approaches for cancer diagnosis and analysis
Abstract Creating fast, non-invasive, precise, and specific diagnostic tests is crucial for enhancing cancer treatment outcomes. Among diagnostic methods, those relying on nucleic acid detection are highly sensitive and specific. Recent developments in diagnostic technologies, particularly those lev...
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| Format: | Article |
| Language: | English |
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BMC
2024-12-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | https://doi.org/10.1186/s12967-024-05908-y |
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| author | Zahra Farshchian Yazdi Shahrzad Roshannezhad Samaneh Sharif Mohammad Reza Abbaszadegan |
| author_facet | Zahra Farshchian Yazdi Shahrzad Roshannezhad Samaneh Sharif Mohammad Reza Abbaszadegan |
| author_sort | Zahra Farshchian Yazdi |
| collection | DOAJ |
| description | Abstract Creating fast, non-invasive, precise, and specific diagnostic tests is crucial for enhancing cancer treatment outcomes. Among diagnostic methods, those relying on nucleic acid detection are highly sensitive and specific. Recent developments in diagnostic technologies, particularly those leveraging Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), are revolutionizing cancer detection, providing accurate and timely results. In clinical oncology, liquid biopsy has become a noninvasive and early-detectable alternative to traditional biopsies over the last two decades. Analyzing the nucleic acid content of liquid biopsy samples, which include Circulating Tumor Cells (CTCs), Circulating Tumor DNA (ctDNA), Circulating Cell-Free RNA (cfRNA), and tumor extracellular vesicles, provides a noninvasive method for cancer detection and monitoring. In this review, we explore how the characteristics of various Cas (CRISPR-associated) enzymes have been utilized in diagnostic assays for cancer liquid biopsy and highlight their main applications of innovative approaches in monitoring, as well as early and rapid detection of cancers. |
| format | Article |
| id | doaj-art-0a97efec7ea342f5ad5ad8f64e070c7a |
| institution | Kabale University |
| issn | 1479-5876 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj-art-0a97efec7ea342f5ad5ad8f64e070c7a2025-01-05T12:44:21ZengBMCJournal of Translational Medicine1479-58762024-12-0122112710.1186/s12967-024-05908-yRecent progress in prompt molecular detection of liquid biopsy using Cas enzymes: innovative approaches for cancer diagnosis and analysisZahra Farshchian Yazdi0Shahrzad Roshannezhad1Samaneh Sharif2Mohammad Reza Abbaszadegan3Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical SciencesDepartment of Science, Mashhad Branch, Islamic Azad UniversityMedical Genetics Research Center, Mashhad University of Medical SciencesDepartment of Medical Genetics, Faculty of Medicine, Mashhad University of Medical SciencesAbstract Creating fast, non-invasive, precise, and specific diagnostic tests is crucial for enhancing cancer treatment outcomes. Among diagnostic methods, those relying on nucleic acid detection are highly sensitive and specific. Recent developments in diagnostic technologies, particularly those leveraging Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), are revolutionizing cancer detection, providing accurate and timely results. In clinical oncology, liquid biopsy has become a noninvasive and early-detectable alternative to traditional biopsies over the last two decades. Analyzing the nucleic acid content of liquid biopsy samples, which include Circulating Tumor Cells (CTCs), Circulating Tumor DNA (ctDNA), Circulating Cell-Free RNA (cfRNA), and tumor extracellular vesicles, provides a noninvasive method for cancer detection and monitoring. In this review, we explore how the characteristics of various Cas (CRISPR-associated) enzymes have been utilized in diagnostic assays for cancer liquid biopsy and highlight their main applications of innovative approaches in monitoring, as well as early and rapid detection of cancers.https://doi.org/10.1186/s12967-024-05908-yCRISPR-CasCancerLiquid biopsyDiagnosis |
| spellingShingle | Zahra Farshchian Yazdi Shahrzad Roshannezhad Samaneh Sharif Mohammad Reza Abbaszadegan Recent progress in prompt molecular detection of liquid biopsy using Cas enzymes: innovative approaches for cancer diagnosis and analysis Journal of Translational Medicine CRISPR-Cas Cancer Liquid biopsy Diagnosis |
| title | Recent progress in prompt molecular detection of liquid biopsy using Cas enzymes: innovative approaches for cancer diagnosis and analysis |
| title_full | Recent progress in prompt molecular detection of liquid biopsy using Cas enzymes: innovative approaches for cancer diagnosis and analysis |
| title_fullStr | Recent progress in prompt molecular detection of liquid biopsy using Cas enzymes: innovative approaches for cancer diagnosis and analysis |
| title_full_unstemmed | Recent progress in prompt molecular detection of liquid biopsy using Cas enzymes: innovative approaches for cancer diagnosis and analysis |
| title_short | Recent progress in prompt molecular detection of liquid biopsy using Cas enzymes: innovative approaches for cancer diagnosis and analysis |
| title_sort | recent progress in prompt molecular detection of liquid biopsy using cas enzymes innovative approaches for cancer diagnosis and analysis |
| topic | CRISPR-Cas Cancer Liquid biopsy Diagnosis |
| url | https://doi.org/10.1186/s12967-024-05908-y |
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