Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen

<b>Aim:</b> The aim of this study was to investigate the effects of BME on Kangal dog sperm parameters following the freezethawing process.<p> <b>Materials and Methods:</b> Ejaculates were collected by digital manipulation twice a week. Ejaculates divided into six equal...

Full description

Saved in:
Bibliographic Details
Main Authors: Sukru Gungor, Mustafa Numan Bucak
Format: Article
Language:English
Published: Selcuk University Press
Series:Eurasian Journal of Veterinary Sciences
Subjects:
Online Access:http://eurasianjvetsci.org/pdf.php3?id=1133
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1841564658237964288
author Sukru Gungor
Mustafa Numan Bucak
author_facet Sukru Gungor
Mustafa Numan Bucak
author_sort Sukru Gungor
collection DOAJ
description <b>Aim:</b> The aim of this study was to investigate the effects of BME on Kangal dog sperm parameters following the freezethawing process.<p> <b>Materials and Methods:</b> Ejaculates were collected by digital manipulation twice a week. Ejaculates divided into six equal aliquots, and diluted with Tris-based extender (T) containing 5% glycerol (G) + BME 2.5% (TGB2.5), TG + BME 10% (TGB10), 5% ethylene glycol (E) + BME 2.5% (TEB2.5), TE + BME 10% (TEB10), TG and TE. Subsequently, the ejaculates were cooled to 4°C at 1.5 hours, and stored in liquid nitrogen (~-196°C).<p> <b>Results:</b> The extender supplemented with 10% BME + 5% G resulted in higher progressive motility (23.19%), in comparison to the extenders supplemented with 5% EG (14.08%). Better sperm acrosome integrity was achieved with the use of the extender containing 2.5% BME + 5% G (44.99%), when compared to the use of the extender supplemented with 5% G (33.63%) (P<0.05).<p> <b>Conclusion:</b> BME added to extender, may improve the postthawed sperm parameters on freezing of Kangal dog semen.
format Article
id doaj-art-0a803141aed84c4398ad306e14637fe5
institution Kabale University
issn 1309-6958
2146-1953
language English
publisher Selcuk University Press
record_format Article
series Eurasian Journal of Veterinary Sciences
spelling doaj-art-0a803141aed84c4398ad306e14637fe52025-01-02T22:36:24ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19533231931991133Basal medium eagle solution may improve the post-thaw parameters of kangal dog semenSukru Gungor0Mustafa Numan Bucak1Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, TurkeyDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey<b>Aim:</b> The aim of this study was to investigate the effects of BME on Kangal dog sperm parameters following the freezethawing process.<p> <b>Materials and Methods:</b> Ejaculates were collected by digital manipulation twice a week. Ejaculates divided into six equal aliquots, and diluted with Tris-based extender (T) containing 5% glycerol (G) + BME 2.5% (TGB2.5), TG + BME 10% (TGB10), 5% ethylene glycol (E) + BME 2.5% (TEB2.5), TE + BME 10% (TEB10), TG and TE. Subsequently, the ejaculates were cooled to 4°C at 1.5 hours, and stored in liquid nitrogen (~-196°C).<p> <b>Results:</b> The extender supplemented with 10% BME + 5% G resulted in higher progressive motility (23.19%), in comparison to the extenders supplemented with 5% EG (14.08%). Better sperm acrosome integrity was achieved with the use of the extender containing 2.5% BME + 5% G (44.99%), when compared to the use of the extender supplemented with 5% G (33.63%) (P<0.05).<p> <b>Conclusion:</b> BME added to extender, may improve the postthawed sperm parameters on freezing of Kangal dog semen.http://eurasianjvetsci.org/pdf.php3?id=1133bmeethylene glycolglycerolkangal dog semen
spellingShingle Sukru Gungor
Mustafa Numan Bucak
Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen
Eurasian Journal of Veterinary Sciences
bme
ethylene glycol
glycerol
kangal dog semen
title Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen
title_full Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen
title_fullStr Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen
title_full_unstemmed Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen
title_short Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen
title_sort basal medium eagle solution may improve the post thaw parameters of kangal dog semen
topic bme
ethylene glycol
glycerol
kangal dog semen
url http://eurasianjvetsci.org/pdf.php3?id=1133
work_keys_str_mv AT sukrugungor basalmediumeaglesolutionmayimprovethepostthawparametersofkangaldogsemen
AT mustafanumanbucak basalmediumeaglesolutionmayimprovethepostthawparametersofkangaldogsemen