Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen
<b>Aim:</b> The aim of this study was to investigate the effects of BME on Kangal dog sperm parameters following the freezethawing process.<p> <b>Materials and Methods:</b> Ejaculates were collected by digital manipulation twice a week. Ejaculates divided into six equal...
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Language: | English |
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Selcuk University Press
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Series: | Eurasian Journal of Veterinary Sciences |
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Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1133 |
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author | Sukru Gungor Mustafa Numan Bucak |
author_facet | Sukru Gungor Mustafa Numan Bucak |
author_sort | Sukru Gungor |
collection | DOAJ |
description | <b>Aim:</b> The aim of this study was to investigate the effects of
BME on Kangal dog sperm parameters following the freezethawing
process.<p>
<b>Materials and Methods:</b> Ejaculates were collected by digital
manipulation twice a week. Ejaculates divided into six equal
aliquots, and diluted with Tris-based extender (T) containing
5% glycerol (G) + BME 2.5% (TGB2.5), TG + BME 10%
(TGB10), 5% ethylene glycol (E) + BME 2.5% (TEB2.5), TE +
BME 10% (TEB10), TG and TE. Subsequently, the ejaculates
were cooled to 4°C at 1.5 hours, and stored in liquid nitrogen
(~-196°C).<p>
<b>Results:</b> The extender supplemented with 10% BME + 5% G
resulted in higher progressive motility (23.19%), in comparison
to the extenders supplemented with 5% EG (14.08%).
Better sperm acrosome integrity was achieved with the use
of the extender containing 2.5% BME + 5% G (44.99%), when
compared to the use of the extender supplemented with 5%
G (33.63%) (P<0.05).<p>
<b>Conclusion:</b> BME added to extender, may improve the postthawed
sperm parameters on freezing of Kangal dog semen. |
format | Article |
id | doaj-art-0a803141aed84c4398ad306e14637fe5 |
institution | Kabale University |
issn | 1309-6958 2146-1953 |
language | English |
publisher | Selcuk University Press |
record_format | Article |
series | Eurasian Journal of Veterinary Sciences |
spelling | doaj-art-0a803141aed84c4398ad306e14637fe52025-01-02T22:36:24ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19533231931991133Basal medium eagle solution may improve the post-thaw parameters of kangal dog semenSukru Gungor0Mustafa Numan Bucak1Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, TurkeyDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey<b>Aim:</b> The aim of this study was to investigate the effects of BME on Kangal dog sperm parameters following the freezethawing process.<p> <b>Materials and Methods:</b> Ejaculates were collected by digital manipulation twice a week. Ejaculates divided into six equal aliquots, and diluted with Tris-based extender (T) containing 5% glycerol (G) + BME 2.5% (TGB2.5), TG + BME 10% (TGB10), 5% ethylene glycol (E) + BME 2.5% (TEB2.5), TE + BME 10% (TEB10), TG and TE. Subsequently, the ejaculates were cooled to 4°C at 1.5 hours, and stored in liquid nitrogen (~-196°C).<p> <b>Results:</b> The extender supplemented with 10% BME + 5% G resulted in higher progressive motility (23.19%), in comparison to the extenders supplemented with 5% EG (14.08%). Better sperm acrosome integrity was achieved with the use of the extender containing 2.5% BME + 5% G (44.99%), when compared to the use of the extender supplemented with 5% G (33.63%) (P<0.05).<p> <b>Conclusion:</b> BME added to extender, may improve the postthawed sperm parameters on freezing of Kangal dog semen.http://eurasianjvetsci.org/pdf.php3?id=1133bmeethylene glycolglycerolkangal dog semen |
spellingShingle | Sukru Gungor Mustafa Numan Bucak Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen Eurasian Journal of Veterinary Sciences bme ethylene glycol glycerol kangal dog semen |
title | Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen |
title_full | Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen |
title_fullStr | Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen |
title_full_unstemmed | Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen |
title_short | Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen |
title_sort | basal medium eagle solution may improve the post thaw parameters of kangal dog semen |
topic | bme ethylene glycol glycerol kangal dog semen |
url | http://eurasianjvetsci.org/pdf.php3?id=1133 |
work_keys_str_mv | AT sukrugungor basalmediumeaglesolutionmayimprovethepostthawparametersofkangaldogsemen AT mustafanumanbucak basalmediumeaglesolutionmayimprovethepostthawparametersofkangaldogsemen |