Identification and bioinformatics analysis of the DUS gene in Eimeria media
Abstract This study aims to explore the coding sequence (CDS) of the putative DUS gene in Eimeria media and assess its potential biological functions during the parasite's lifecycle. Initially, oocysts were isolated from fecal samples of rabbits infected with E. media, from which DNA and RNA we...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-024-04362-8 |
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| author | Yijin Zou Yiyan Wang Diyi Duan Xun Suo Yuanyuan Zhang |
| author_facet | Yijin Zou Yiyan Wang Diyi Duan Xun Suo Yuanyuan Zhang |
| author_sort | Yijin Zou |
| collection | DOAJ |
| description | Abstract This study aims to explore the coding sequence (CDS) of the putative DUS gene in Eimeria media and assess its potential biological functions during the parasite's lifecycle. Initially, oocysts were isolated from fecal samples of rabbits infected with E. media, from which DNA and RNA were extracted. These extractions were used as templates for PCR to successfully amplify the CDS of the DUS gene, confirming its presence within the E. media genome. Further analysis using quantitative PCR (qPCR) demonstrated significantly higher expression of the DUS gene in the precocious line (PL) compared to the wild type (WT). This differential expression highlights a potential functional role for the DUS gene in influencing the development and sporulation processes of E. media, which may contribute to the precocious phenotype. Additionally, bioinformatics analysis provided insights into the evolutionary trends and structural characteristics of the DUS gene across different species, suggesting a broader biological significance. The elevated expression of the DUS gene in the PL suggests its critical involvement in the growth and reproductive processes of the parasite. This finding opens new avenues for research aimed at controlling E. media infection through targeted interventions in the DUS gene expression pathways. |
| format | Article |
| id | doaj-art-0a7f8da742744d0cac811da7103e87f5 |
| institution | Kabale University |
| issn | 1746-6148 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj-art-0a7f8da742744d0cac811da7103e87f52025-01-12T12:27:55ZengBMCBMC Veterinary Research1746-61482025-01-0121111510.1186/s12917-024-04362-8Identification and bioinformatics analysis of the DUS gene in Eimeria mediaYijin Zou0Yiyan Wang1Diyi Duan2Xun Suo3Yuanyuan Zhang4National Key Laboratory of Veterinary Public Health Security, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural UniversityNational Key Laboratory of Veterinary Public Health Security, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural UniversityNational Key Laboratory of Veterinary Public Health Security, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural UniversityNational Key Laboratory of Veterinary Public Health Security, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural UniversityNational Key Laboratory of Veterinary Public Health Security, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural UniversityAbstract This study aims to explore the coding sequence (CDS) of the putative DUS gene in Eimeria media and assess its potential biological functions during the parasite's lifecycle. Initially, oocysts were isolated from fecal samples of rabbits infected with E. media, from which DNA and RNA were extracted. These extractions were used as templates for PCR to successfully amplify the CDS of the DUS gene, confirming its presence within the E. media genome. Further analysis using quantitative PCR (qPCR) demonstrated significantly higher expression of the DUS gene in the precocious line (PL) compared to the wild type (WT). This differential expression highlights a potential functional role for the DUS gene in influencing the development and sporulation processes of E. media, which may contribute to the precocious phenotype. Additionally, bioinformatics analysis provided insights into the evolutionary trends and structural characteristics of the DUS gene across different species, suggesting a broader biological significance. The elevated expression of the DUS gene in the PL suggests its critical involvement in the growth and reproductive processes of the parasite. This finding opens new avenues for research aimed at controlling E. media infection through targeted interventions in the DUS gene expression pathways.https://doi.org/10.1186/s12917-024-04362-8DUS geneEimeria mediaPrecocious phenotypeBioinformatics |
| spellingShingle | Yijin Zou Yiyan Wang Diyi Duan Xun Suo Yuanyuan Zhang Identification and bioinformatics analysis of the DUS gene in Eimeria media BMC Veterinary Research DUS gene Eimeria media Precocious phenotype Bioinformatics |
| title | Identification and bioinformatics analysis of the DUS gene in Eimeria media |
| title_full | Identification and bioinformatics analysis of the DUS gene in Eimeria media |
| title_fullStr | Identification and bioinformatics analysis of the DUS gene in Eimeria media |
| title_full_unstemmed | Identification and bioinformatics analysis of the DUS gene in Eimeria media |
| title_short | Identification and bioinformatics analysis of the DUS gene in Eimeria media |
| title_sort | identification and bioinformatics analysis of the dus gene in eimeria media |
| topic | DUS gene Eimeria media Precocious phenotype Bioinformatics |
| url | https://doi.org/10.1186/s12917-024-04362-8 |
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