Evaluation of amoebicidal potential of Paneth cell cryptdin-2 against Entamoeba histolytica.
<h4>Background</h4>Amoebiasis is a major public health problem in tropical and subtropical countries. Currently, metronidazole is the gold choice medication for the treatment of this disease. However, reports have indicated towards the possibility of development of metronidazole-resistan...
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Public Library of Science (PLoS)
2011-12-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0001386&type=printable |
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| author | Simran Preet Sanjay Bharati Geeta Shukla Ashwani Koul Praveen Rishi |
| author_facet | Simran Preet Sanjay Bharati Geeta Shukla Ashwani Koul Praveen Rishi |
| author_sort | Simran Preet |
| collection | DOAJ |
| description | <h4>Background</h4>Amoebiasis is a major public health problem in tropical and subtropical countries. Currently, metronidazole is the gold choice medication for the treatment of this disease. However, reports have indicated towards the possibility of development of metronidazole-resistance in Entamoeba strains in near future. In view of the emergence of this possibility, in addition to the associated side effects and mutagenic ability of the currently available anti-amoebic drugs, there is a need to explore newer therapeutics against this disease. In this context, the present study evaluated the amoebicidal potential of cryptdin-2 against E. histolytica.<h4>Methods/principal findings</h4>In the present study, cryptdin-2 exhibited potent in-vitro amoebicidal activity against E. histolytica in a concentration dependent manner at a minimum amoebicidal concentration (MAC) of 4 mg/L. Scanning electron microscopy as well as phase contrast microscopic investigations of cryptdin-2 treated trophozoites revealed that the peptide was able to induce significant morphological alterations in terms of membrane wrinkling, leakage of the cytoplasmic contents and damaged plasma membrane suggesting a possible membrane dependent amoebicidal activity. N-phenyl napthylamine (NPN) uptake assay in presence of sulethal, lethal as well as twice the lethal concentrations further confirmed the membrane-dependent mode of action of cryptdin-2 and suggested that the peptide could permeabilize the plasma membrane of E. histolytica. It was also found that cryptdin-2 interfered with DNA, RNA as well as protein synthesis of E. histolytica exerting the highest effect against DNA synthesis. Thus, the macromolecular synthesis studies correlated well with the observations of membrane permeabilization studies.<h4>Significance/conclusions</h4>The amoebicidal efficacy of cryptdin-2 suggests that it may be exploited as a promising option to combat amoebiasis or, at least, may act as an adjunct to metronidazole and/or other available anti-amoebic drugs. |
| format | Article |
| id | doaj-art-0a62f2b2a2254fb6a085c2e10e1ab0ec |
| institution | Kabale University |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2011-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-0a62f2b2a2254fb6a085c2e10e1ab0ec2025-01-16T05:32:28ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352011-12-01512e138610.1371/journal.pntd.0001386Evaluation of amoebicidal potential of Paneth cell cryptdin-2 against Entamoeba histolytica.Simran PreetSanjay BharatiGeeta ShuklaAshwani KoulPraveen Rishi<h4>Background</h4>Amoebiasis is a major public health problem in tropical and subtropical countries. Currently, metronidazole is the gold choice medication for the treatment of this disease. However, reports have indicated towards the possibility of development of metronidazole-resistance in Entamoeba strains in near future. In view of the emergence of this possibility, in addition to the associated side effects and mutagenic ability of the currently available anti-amoebic drugs, there is a need to explore newer therapeutics against this disease. In this context, the present study evaluated the amoebicidal potential of cryptdin-2 against E. histolytica.<h4>Methods/principal findings</h4>In the present study, cryptdin-2 exhibited potent in-vitro amoebicidal activity against E. histolytica in a concentration dependent manner at a minimum amoebicidal concentration (MAC) of 4 mg/L. Scanning electron microscopy as well as phase contrast microscopic investigations of cryptdin-2 treated trophozoites revealed that the peptide was able to induce significant morphological alterations in terms of membrane wrinkling, leakage of the cytoplasmic contents and damaged plasma membrane suggesting a possible membrane dependent amoebicidal activity. N-phenyl napthylamine (NPN) uptake assay in presence of sulethal, lethal as well as twice the lethal concentrations further confirmed the membrane-dependent mode of action of cryptdin-2 and suggested that the peptide could permeabilize the plasma membrane of E. histolytica. It was also found that cryptdin-2 interfered with DNA, RNA as well as protein synthesis of E. histolytica exerting the highest effect against DNA synthesis. Thus, the macromolecular synthesis studies correlated well with the observations of membrane permeabilization studies.<h4>Significance/conclusions</h4>The amoebicidal efficacy of cryptdin-2 suggests that it may be exploited as a promising option to combat amoebiasis or, at least, may act as an adjunct to metronidazole and/or other available anti-amoebic drugs.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0001386&type=printable |
| spellingShingle | Simran Preet Sanjay Bharati Geeta Shukla Ashwani Koul Praveen Rishi Evaluation of amoebicidal potential of Paneth cell cryptdin-2 against Entamoeba histolytica. PLoS Neglected Tropical Diseases |
| title | Evaluation of amoebicidal potential of Paneth cell cryptdin-2 against Entamoeba histolytica. |
| title_full | Evaluation of amoebicidal potential of Paneth cell cryptdin-2 against Entamoeba histolytica. |
| title_fullStr | Evaluation of amoebicidal potential of Paneth cell cryptdin-2 against Entamoeba histolytica. |
| title_full_unstemmed | Evaluation of amoebicidal potential of Paneth cell cryptdin-2 against Entamoeba histolytica. |
| title_short | Evaluation of amoebicidal potential of Paneth cell cryptdin-2 against Entamoeba histolytica. |
| title_sort | evaluation of amoebicidal potential of paneth cell cryptdin 2 against entamoeba histolytica |
| url | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0001386&type=printable |
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