Characterization of Elovl2/5 in abalone Haliotis discus hannai Ino
Elongation of very long-chain fatty acids protein (Elovl) plays vital role in the biosynthesis of long chain polyunsaturated fatty acids (LC-PUFA) in aquatic animals. However, there is very limited research on Elovl in abalone. In this study, an elongase gene encoding 303 amino acids was found to ex...
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Elsevier
2025-09-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425002467 |
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| author | Mingli Shao Wei Chen Lina Zhu Shikai Liu Óscar Monroig Juan Carlos Navarro Chunnuan Zhao Junsong YangLi Mingzhu Li Xiaotong Wang |
| author_facet | Mingli Shao Wei Chen Lina Zhu Shikai Liu Óscar Monroig Juan Carlos Navarro Chunnuan Zhao Junsong YangLi Mingzhu Li Xiaotong Wang |
| author_sort | Mingli Shao |
| collection | DOAJ |
| description | Elongation of very long-chain fatty acids protein (Elovl) plays vital role in the biosynthesis of long chain polyunsaturated fatty acids (LC-PUFA) in aquatic animals. However, there is very limited research on Elovl in abalone. In this study, an elongase gene encoding 303 amino acids was found to exhibit higher homology with the Elovl2/5 sequences of mollusks (60 %) than the Elovl5 of fish and mammals (about 43.30 %). Heterologous expression in yeast showed that abalone Elovl could efficiently catalyze the extension of C18:3n-3, C18:3n-6 and C18:4n-3, moderately elongate C18:2n-6, C20:4n-6 and C20:5n-3 to corresponding products. However, it has extremely weak ability to extend C20:3n-6 and C20:4n-3 and no affinity for C22 PUFA. Phylogenetic tree analysis showed that the sequence of abalone Elovl is closely related to Elovl2/5 of the cuttlefish (Sepia officinalis) and octopus (Octopus vulgaris). Tissue distribution revealed that the Elovl was highly expressed in female and male gonads, moderately in hepatopancreas, and minimally in other tested tissues. The expression levels of Elovl in hepatopancreas of abalone fed 50 % FO and 75 % FO diets were significantly higher than that in the 100 % FO control group. And no significant differences were found in the expressions of 0 % FO, 25 % FO and control groups. The EPA in 75 % FO group is significantly higher than those of other groups. This study provides the first systematic characterization of the dual substrate specificity of Elovl in abalone, bridging a critical knowledge gap in the evolutionary and functional investigation of elongases within gastropods. |
| format | Article |
| id | doaj-art-0a383d9e19d44d169b46aa1ef3480f4c |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-0a383d9e19d44d169b46aa1ef3480f4c2025-08-20T03:45:10ZengElsevierAquaculture Reports2352-51342025-09-014310286010.1016/j.aqrep.2025.102860Characterization of Elovl2/5 in abalone Haliotis discus hannai InoMingli Shao0Wei Chen1Lina Zhu2Shikai Liu3Óscar Monroig4Juan Carlos Navarro5Chunnuan Zhao6Junsong YangLi7Mingzhu Li8Xiaotong Wang9School of Fisheries, Ludong University, Yantai, PR ChinaYantai Marine Economic Research Institute, Yantai, PR ChinaSchool of Fisheries, Ludong University, Yantai, PR ChinaKey Laboratory of Mariculture (Ocean University of China), Ministry of Education, and College of Fisheries, Ocean University of China, Qingdao 266003, PR ChinaInstituto de Acuicultura Torre de la Sal (IATS), CSIC, Ribera de Cabanes, Castellón 12595, SpainInstituto de Acuicultura Torre de la Sal (IATS), CSIC, Ribera de Cabanes, Castellón 12595, SpainChangdao Enhancement and Experiment Station, Changdao, PR ChinaSchool of Fisheries, Ludong University, Yantai, PR ChinaSchool of Fisheries, Ludong University, Yantai, PR China; Corresponding authors.School of Fisheries, Ludong University, Yantai, PR China; Corresponding authors.Elongation of very long-chain fatty acids protein (Elovl) plays vital role in the biosynthesis of long chain polyunsaturated fatty acids (LC-PUFA) in aquatic animals. However, there is very limited research on Elovl in abalone. In this study, an elongase gene encoding 303 amino acids was found to exhibit higher homology with the Elovl2/5 sequences of mollusks (60 %) than the Elovl5 of fish and mammals (about 43.30 %). Heterologous expression in yeast showed that abalone Elovl could efficiently catalyze the extension of C18:3n-3, C18:3n-6 and C18:4n-3, moderately elongate C18:2n-6, C20:4n-6 and C20:5n-3 to corresponding products. However, it has extremely weak ability to extend C20:3n-6 and C20:4n-3 and no affinity for C22 PUFA. Phylogenetic tree analysis showed that the sequence of abalone Elovl is closely related to Elovl2/5 of the cuttlefish (Sepia officinalis) and octopus (Octopus vulgaris). Tissue distribution revealed that the Elovl was highly expressed in female and male gonads, moderately in hepatopancreas, and minimally in other tested tissues. The expression levels of Elovl in hepatopancreas of abalone fed 50 % FO and 75 % FO diets were significantly higher than that in the 100 % FO control group. And no significant differences were found in the expressions of 0 % FO, 25 % FO and control groups. The EPA in 75 % FO group is significantly higher than those of other groups. This study provides the first systematic characterization of the dual substrate specificity of Elovl in abalone, bridging a critical knowledge gap in the evolutionary and functional investigation of elongases within gastropods.http://www.sciencedirect.com/science/article/pii/S2352513425002467ElongaseAbaloneLC-PUFANutritional regulationFatty acid |
| spellingShingle | Mingli Shao Wei Chen Lina Zhu Shikai Liu Óscar Monroig Juan Carlos Navarro Chunnuan Zhao Junsong YangLi Mingzhu Li Xiaotong Wang Characterization of Elovl2/5 in abalone Haliotis discus hannai Ino Aquaculture Reports Elongase Abalone LC-PUFA Nutritional regulation Fatty acid |
| title | Characterization of Elovl2/5 in abalone Haliotis discus hannai Ino |
| title_full | Characterization of Elovl2/5 in abalone Haliotis discus hannai Ino |
| title_fullStr | Characterization of Elovl2/5 in abalone Haliotis discus hannai Ino |
| title_full_unstemmed | Characterization of Elovl2/5 in abalone Haliotis discus hannai Ino |
| title_short | Characterization of Elovl2/5 in abalone Haliotis discus hannai Ino |
| title_sort | characterization of elovl2 5 in abalone haliotis discus hannai ino |
| topic | Elongase Abalone LC-PUFA Nutritional regulation Fatty acid |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425002467 |
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