Estimation of Throughfall and Stemflow Bacterial Flux in a Subtropical Oak‐Cedar Forest

Abstract Transport pathways of microbes between ecosystem spheres (atmosphere, phyllosphere, and pedosphere) represent major fluxes in nutrient cycles and have the potential to affect microbially mediated biogeochemical processes. Novel data on bacterial fluxes from the phyllosphere to the pedospher...

Full description

Saved in:
Bibliographic Details
Main Authors: Thais B. Bittar, Preston Pound, Ansley Whitetree, L. Dean Moore, John T. Van Stan II
Format: Article
Language:English
Published: Wiley 2018-02-01
Series:Geophysical Research Letters
Subjects:
Online Access:https://doi.org/10.1002/2017GL075827
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849321379148595200
author Thais B. Bittar
Preston Pound
Ansley Whitetree
L. Dean Moore
John T. Van Stan II
author_facet Thais B. Bittar
Preston Pound
Ansley Whitetree
L. Dean Moore
John T. Van Stan II
author_sort Thais B. Bittar
collection DOAJ
description Abstract Transport pathways of microbes between ecosystem spheres (atmosphere, phyllosphere, and pedosphere) represent major fluxes in nutrient cycles and have the potential to affect microbially mediated biogeochemical processes. Novel data on bacterial fluxes from the phyllosphere to the pedosphere during rainfall via throughfall (rain dripping from/through the canopy) and stemflow (rain funneled down tree stems) are reported. Bacterial concentrations were quantified using flow cytometry and validated with quantitative polymerase chain reaction assays in rainfall samples from an oak‐cedar forest in coastal Georgia (southeastern U.S.). Bacteria concentrations (cells mL−1) and storm‐normalized fluxes (cells m−2 h−1, cells m−2 mm−1) were greater for cedar versus oak. Total bacterial flux was 1.5 × 1016 cells ha−1 yr−1. These previously unexamined bacterial fluxes are interpreted in the context of major elemental pools and fluxes in forests and could represent inoculum‐level sources of bacteria (if alive), and organic matter and inorganic solute inputs (if lysed) to soils.
format Article
id doaj-art-0a1d32a8547d4bcd87c2d26e55017ea6
institution Kabale University
issn 0094-8276
1944-8007
language English
publishDate 2018-02-01
publisher Wiley
record_format Article
series Geophysical Research Letters
spelling doaj-art-0a1d32a8547d4bcd87c2d26e55017ea62025-08-20T03:49:46ZengWileyGeophysical Research Letters0094-82761944-80072018-02-014531410141810.1002/2017GL075827Estimation of Throughfall and Stemflow Bacterial Flux in a Subtropical Oak‐Cedar ForestThais B. Bittar0Preston Pound1Ansley Whitetree2L. Dean Moore3John T. Van Stan II4Skidaway Institute of Oceanography, Marine Sciences Department University of Georgia Savannah GA USADepartment of Geology and Geography Georgia Southern University Statesboro GA USADepartment of Geology and Geography Georgia Southern University Statesboro GA USADepartment of Geology and Geography Georgia Southern University Statesboro GA USADepartment of Geology and Geography Georgia Southern University Statesboro GA USAAbstract Transport pathways of microbes between ecosystem spheres (atmosphere, phyllosphere, and pedosphere) represent major fluxes in nutrient cycles and have the potential to affect microbially mediated biogeochemical processes. Novel data on bacterial fluxes from the phyllosphere to the pedosphere during rainfall via throughfall (rain dripping from/through the canopy) and stemflow (rain funneled down tree stems) are reported. Bacterial concentrations were quantified using flow cytometry and validated with quantitative polymerase chain reaction assays in rainfall samples from an oak‐cedar forest in coastal Georgia (southeastern U.S.). Bacteria concentrations (cells mL−1) and storm‐normalized fluxes (cells m−2 h−1, cells m−2 mm−1) were greater for cedar versus oak. Total bacterial flux was 1.5 × 1016 cells ha−1 yr−1. These previously unexamined bacterial fluxes are interpreted in the context of major elemental pools and fluxes in forests and could represent inoculum‐level sources of bacteria (if alive), and organic matter and inorganic solute inputs (if lysed) to soils.https://doi.org/10.1002/2017GL075827bacteriathroughfallstemflowQuercus virginianaJuniperus virginianaflow cytometry
spellingShingle Thais B. Bittar
Preston Pound
Ansley Whitetree
L. Dean Moore
John T. Van Stan II
Estimation of Throughfall and Stemflow Bacterial Flux in a Subtropical Oak‐Cedar Forest
Geophysical Research Letters
bacteria
throughfall
stemflow
Quercus virginiana
Juniperus virginiana
flow cytometry
title Estimation of Throughfall and Stemflow Bacterial Flux in a Subtropical Oak‐Cedar Forest
title_full Estimation of Throughfall and Stemflow Bacterial Flux in a Subtropical Oak‐Cedar Forest
title_fullStr Estimation of Throughfall and Stemflow Bacterial Flux in a Subtropical Oak‐Cedar Forest
title_full_unstemmed Estimation of Throughfall and Stemflow Bacterial Flux in a Subtropical Oak‐Cedar Forest
title_short Estimation of Throughfall and Stemflow Bacterial Flux in a Subtropical Oak‐Cedar Forest
title_sort estimation of throughfall and stemflow bacterial flux in a subtropical oak cedar forest
topic bacteria
throughfall
stemflow
Quercus virginiana
Juniperus virginiana
flow cytometry
url https://doi.org/10.1002/2017GL075827
work_keys_str_mv AT thaisbbittar estimationofthroughfallandstemflowbacterialfluxinasubtropicaloakcedarforest
AT prestonpound estimationofthroughfallandstemflowbacterialfluxinasubtropicaloakcedarforest
AT ansleywhitetree estimationofthroughfallandstemflowbacterialfluxinasubtropicaloakcedarforest
AT ldeanmoore estimationofthroughfallandstemflowbacterialfluxinasubtropicaloakcedarforest
AT johntvanstanii estimationofthroughfallandstemflowbacterialfluxinasubtropicaloakcedarforest