<i>OsABT</i> Negatively Regulates the Abscisic Acid Signal Transduction Pathway in Rice Seedling Roots
Rice (<i>Oryza sativa</i> L.) is a main food crop in China and is crucial for the maintenance of national food security. The growth of rice seedling roots is regulated by a variety of genes and is closely related to abscisic acid (ABA) metabolism and ABA signaling pathways. In this study...
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MDPI AG
2024-11-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/14/11/2683 |
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| author | Lingran Bao Yi Shen Yijie Yan Xuanzhu Huang Danni Wen Bo Shen |
| author_facet | Lingran Bao Yi Shen Yijie Yan Xuanzhu Huang Danni Wen Bo Shen |
| author_sort | Lingran Bao |
| collection | DOAJ |
| description | Rice (<i>Oryza sativa</i> L.) is a main food crop in China and is crucial for the maintenance of national food security. The growth of rice seedling roots is regulated by a variety of genes and is closely related to abscisic acid (ABA) metabolism and ABA signaling pathways. In this study, we found that <i>OsABT</i> could increase the length of rice root tip meristem cells and upregulate root development-related genes, thereby alleviating ABA’s inhibitory effects on rice root growth and seed germination. The overexpression of <i>OsABT</i> reduced the ABA content by downregulating ABA synthesis genes (<i>OsNCED3</i> and <i>OsNCED5</i>) and upregulating the ABA catabolic gene (<i>OsABA8ox2</i>). In addition, <i>OsABT</i> interacted with OsPYL4, OsPYL10, and OsABIL2 via the ABA signal transduction pathway. By inhibiting the expression of positive regulatory genes (<i>OsPYL9</i> and <i>Rab16a</i>) and increasing the expression of a negative regulatory gene (<i>OsABIL1</i>), <i>OsABT</i> negatively regulates the ABA signal transduction pathway. Transcriptome analysis revealed that <i>OsABT</i> inhibited the activity of Gene Ontology entries in response to ABA. Thus, <i>OsABT</i> increased the length of the rice root meristem, reduced the accumulation of ABA in the roots, and negatively regulated the ABA signal transduction pathway by interacting with key proteins in this pathway, ultimately relieving the inhibitory effect of ABA on rice root length and seed germination. |
| format | Article |
| id | doaj-art-0a189a3397544d928f1a17d4716f636f |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-0a189a3397544d928f1a17d4716f636f2024-11-26T17:44:59ZengMDPI AGAgronomy2073-43952024-11-011411268310.3390/agronomy14112683<i>OsABT</i> Negatively Regulates the Abscisic Acid Signal Transduction Pathway in Rice Seedling RootsLingran Bao0Yi Shen1Yijie Yan2Xuanzhu Huang3Danni Wen4Bo Shen5College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaRice (<i>Oryza sativa</i> L.) is a main food crop in China and is crucial for the maintenance of national food security. The growth of rice seedling roots is regulated by a variety of genes and is closely related to abscisic acid (ABA) metabolism and ABA signaling pathways. In this study, we found that <i>OsABT</i> could increase the length of rice root tip meristem cells and upregulate root development-related genes, thereby alleviating ABA’s inhibitory effects on rice root growth and seed germination. The overexpression of <i>OsABT</i> reduced the ABA content by downregulating ABA synthesis genes (<i>OsNCED3</i> and <i>OsNCED5</i>) and upregulating the ABA catabolic gene (<i>OsABA8ox2</i>). In addition, <i>OsABT</i> interacted with OsPYL4, OsPYL10, and OsABIL2 via the ABA signal transduction pathway. By inhibiting the expression of positive regulatory genes (<i>OsPYL9</i> and <i>Rab16a</i>) and increasing the expression of a negative regulatory gene (<i>OsABIL1</i>), <i>OsABT</i> negatively regulates the ABA signal transduction pathway. Transcriptome analysis revealed that <i>OsABT</i> inhibited the activity of Gene Ontology entries in response to ABA. Thus, <i>OsABT</i> increased the length of the rice root meristem, reduced the accumulation of ABA in the roots, and negatively regulated the ABA signal transduction pathway by interacting with key proteins in this pathway, ultimately relieving the inhibitory effect of ABA on rice root length and seed germination.https://www.mdpi.com/2073-4395/14/11/2683ricerootABAfluridonesignal transduction pathwaytranscriptome |
| spellingShingle | Lingran Bao Yi Shen Yijie Yan Xuanzhu Huang Danni Wen Bo Shen <i>OsABT</i> Negatively Regulates the Abscisic Acid Signal Transduction Pathway in Rice Seedling Roots Agronomy rice root ABA fluridone signal transduction pathway transcriptome |
| title | <i>OsABT</i> Negatively Regulates the Abscisic Acid Signal Transduction Pathway in Rice Seedling Roots |
| title_full | <i>OsABT</i> Negatively Regulates the Abscisic Acid Signal Transduction Pathway in Rice Seedling Roots |
| title_fullStr | <i>OsABT</i> Negatively Regulates the Abscisic Acid Signal Transduction Pathway in Rice Seedling Roots |
| title_full_unstemmed | <i>OsABT</i> Negatively Regulates the Abscisic Acid Signal Transduction Pathway in Rice Seedling Roots |
| title_short | <i>OsABT</i> Negatively Regulates the Abscisic Acid Signal Transduction Pathway in Rice Seedling Roots |
| title_sort | i osabt i negatively regulates the abscisic acid signal transduction pathway in rice seedling roots |
| topic | rice root ABA fluridone signal transduction pathway transcriptome |
| url | https://www.mdpi.com/2073-4395/14/11/2683 |
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