Quantitative real-time PCR analysis of gut microbiota in rheumatoid arthritis patients compared to healthy controls
Abstract Rheumatoid arthritis (RA) is an autoimmune disorder with synovial inflammation of joints and extra articular manifestations. The results of recent researches consider the relationship between microbiota and the immune system as a double-edged sword. Considering that the relationship between...
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SpringerOpen
2024-12-01
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| Online Access: | https://doi.org/10.1186/s13568-024-01785-2 |
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| author | Azin Masroor Abolfazl Gholipour Milad Shahini Shams Abadi Mohammad Mousavi Moluk Hadi Alijanvand Behnam Zamanzad |
| author_facet | Azin Masroor Abolfazl Gholipour Milad Shahini Shams Abadi Mohammad Mousavi Moluk Hadi Alijanvand Behnam Zamanzad |
| author_sort | Azin Masroor |
| collection | DOAJ |
| description | Abstract Rheumatoid arthritis (RA) is an autoimmune disorder with synovial inflammation of joints and extra articular manifestations. The results of recent researches consider the relationship between microbiota and the immune system as a double-edged sword. Considering that the relationship between the composition of intestinal microbiota and the immunological and clinical status of the body has been confirmed, it is very important to investigate the effect of each genus and species of bacteria on the state of the immune system. The current study was determined using 16S rRNA gene sequencing to explore the 4 selected gut microbiota from 25 people suffering from rheumatism (RA group) with a time interval of at least 3 years from the onset of the disease and 25 Healthy people by real time PCR. Gut dysbiosis in patients with rheumatoid arthritis (RA) is identified alongside key serological and clinical markers such as C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), immunofluorescence (IF), anti-cyclic citrullinated peptide (Anti-CCP), white blood cell count (WBC), mean corpuscular volume (MCV), aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), Platelet count (PLT), thyroid-stimulating hormone (TSH), creatinine (Cr), and hemoglobin (Hb). Additionally, data from individuals with incomplete or unverified records were excluded from the study to ensure accuracy and reliability. Bacteroides fragilis, Roseburia faecis and Fusobacterium nucleatum genera showed a much lower median in Rheumatoid arthritis patients in comparison with healthy people (P > 0.001, p = 0.002, P < 0.001 respectively). While the difference in the median of E. coli genera was not significant in the two studied groups (p = 0.31). In such a way that the change in the Gut normal flora homeostasis and the reduction of beneficial bacteria such as Bacteroides fragilis, Roseburia faecis, Fusobacterium nucleatum genera, may stimulate the immune system to initiate autoimmunity. |
| format | Article |
| id | doaj-art-09dbbcd7092542b58eb75f1cb2e8d3a6 |
| institution | Kabale University |
| issn | 2191-0855 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj-art-09dbbcd7092542b58eb75f1cb2e8d3a62024-12-22T12:49:43ZengSpringerOpenAMB Express2191-08552024-12-0114111210.1186/s13568-024-01785-2Quantitative real-time PCR analysis of gut microbiota in rheumatoid arthritis patients compared to healthy controlsAzin Masroor0Abolfazl Gholipour1Milad Shahini Shams Abadi2Mohammad Mousavi3Moluk Hadi Alijanvand4Behnam Zamanzad5Department of Medical Microbiology and Immunology, Faculty of Medicine, Shahrekord Medical UniversityDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Shahrekord Medical UniversityDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Shahrekord Medical UniversityDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Shahrekord Medical UniversityDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Shahrekord Medical UniversityDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Shahrekord Medical UniversityAbstract Rheumatoid arthritis (RA) is an autoimmune disorder with synovial inflammation of joints and extra articular manifestations. The results of recent researches consider the relationship between microbiota and the immune system as a double-edged sword. Considering that the relationship between the composition of intestinal microbiota and the immunological and clinical status of the body has been confirmed, it is very important to investigate the effect of each genus and species of bacteria on the state of the immune system. The current study was determined using 16S rRNA gene sequencing to explore the 4 selected gut microbiota from 25 people suffering from rheumatism (RA group) with a time interval of at least 3 years from the onset of the disease and 25 Healthy people by real time PCR. Gut dysbiosis in patients with rheumatoid arthritis (RA) is identified alongside key serological and clinical markers such as C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), immunofluorescence (IF), anti-cyclic citrullinated peptide (Anti-CCP), white blood cell count (WBC), mean corpuscular volume (MCV), aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), Platelet count (PLT), thyroid-stimulating hormone (TSH), creatinine (Cr), and hemoglobin (Hb). Additionally, data from individuals with incomplete or unverified records were excluded from the study to ensure accuracy and reliability. Bacteroides fragilis, Roseburia faecis and Fusobacterium nucleatum genera showed a much lower median in Rheumatoid arthritis patients in comparison with healthy people (P > 0.001, p = 0.002, P < 0.001 respectively). While the difference in the median of E. coli genera was not significant in the two studied groups (p = 0.31). In such a way that the change in the Gut normal flora homeostasis and the reduction of beneficial bacteria such as Bacteroides fragilis, Roseburia faecis, Fusobacterium nucleatum genera, may stimulate the immune system to initiate autoimmunity.https://doi.org/10.1186/s13568-024-01785-2MicrobiotaDysbiosisRheumatoid arthritisAuto-immune |
| spellingShingle | Azin Masroor Abolfazl Gholipour Milad Shahini Shams Abadi Mohammad Mousavi Moluk Hadi Alijanvand Behnam Zamanzad Quantitative real-time PCR analysis of gut microbiota in rheumatoid arthritis patients compared to healthy controls AMB Express Microbiota Dysbiosis Rheumatoid arthritis Auto-immune |
| title | Quantitative real-time PCR analysis of gut microbiota in rheumatoid arthritis patients compared to healthy controls |
| title_full | Quantitative real-time PCR analysis of gut microbiota in rheumatoid arthritis patients compared to healthy controls |
| title_fullStr | Quantitative real-time PCR analysis of gut microbiota in rheumatoid arthritis patients compared to healthy controls |
| title_full_unstemmed | Quantitative real-time PCR analysis of gut microbiota in rheumatoid arthritis patients compared to healthy controls |
| title_short | Quantitative real-time PCR analysis of gut microbiota in rheumatoid arthritis patients compared to healthy controls |
| title_sort | quantitative real time pcr analysis of gut microbiota in rheumatoid arthritis patients compared to healthy controls |
| topic | Microbiota Dysbiosis Rheumatoid arthritis Auto-immune |
| url | https://doi.org/10.1186/s13568-024-01785-2 |
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