<i>L. plantarum</i> UBLP-40 Versus the Combined Formula of <i>L. rhamnosus</i> UBLP-58 and <i>B. longum</i> UBBL-64 in Excisional Wound Healing: A Cellular Perspective
Introduction: The utilization of probiotics in enhancing the active healing of skin wounds represents a burgeoning trend in contemporary medicine. Previous research has extensively explored wound healing mechanisms involving the strains of <i>Lactiplantibacillus plantarum</i>, <i>L...
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2024-10-01
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| author | Moysis Moysidis Angeliki Chorti Angeliki Cheva Ioanna Abba Deka Georgios Tzikos Christoforos Kosmidis Ioannis Koutelidakis Joulia K. Tsetis Theodossis Papavramidis Katerina Kotzampassi |
| author_facet | Moysis Moysidis Angeliki Chorti Angeliki Cheva Ioanna Abba Deka Georgios Tzikos Christoforos Kosmidis Ioannis Koutelidakis Joulia K. Tsetis Theodossis Papavramidis Katerina Kotzampassi |
| author_sort | Moysis Moysidis |
| collection | DOAJ |
| description | Introduction: The utilization of probiotics in enhancing the active healing of skin wounds represents a burgeoning trend in contemporary medicine. Previous research has extensively explored wound healing mechanisms involving the strains of <i>Lactiplantibacillus plantarum</i>, <i>Lacticaseibacillus rhamnosus,</i> and <i>Bifidobacterium longum</i>. This study seeks to compare and interpret cellular findings derived from immunohistochemical and pathological applications. Methods: Three groups (the control, <i>Lactiplantibacillus plantarum</i> (RO1) group, and <i>Lacticaseibacillus rhamnosus</i> and <i>Bifidobacterium longum</i> (PRO2) group) underwent histological analysis, and microscopic cell counting were employed, offering insights into dynamic changes among neutrophils, lymphocytes, plasmacytes, mast cells, fibroblasts, and newly formed vessels across distinct treatment groups and temporal intervals. Results: The neutrophil count was found to be elevated in PRO2 on day 2, while the same group resulted in the highest decline on day 15. The number of fibroblasts peaked on day 4 for the PRO1 group, compared to the other two groups, which peaked on day 8. The lymphocyte count was the highest in the control group, while they peaked on day 4 in PRO2. The mast cells and plasmacytes were variable and sparse among all groups and time frames. Neovascularization was promoted by PRO1 and PRO2 groups on day 4 and remained high on day 8 for PRO2. Conclusions: Probiotic strains can be beneficial to the human population and in assisting skin wound healing, each strain working differently and more effectively in different healing phases. Thus, a combined formula containing different probiotics to modulate various healing phases is desirable. |
| format | Article |
| id | doaj-art-09646622609945e4a78dd8984e529e7f |
| institution | Kabale University |
| issn | 1424-8247 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Pharmaceuticals |
| spelling | doaj-art-09646622609945e4a78dd8984e529e7f2024-11-26T18:17:04ZengMDPI AGPharmaceuticals1424-82472024-10-011711141410.3390/ph17111414<i>L. plantarum</i> UBLP-40 Versus the Combined Formula of <i>L. rhamnosus</i> UBLP-58 and <i>B. longum</i> UBBL-64 in Excisional Wound Healing: A Cellular PerspectiveMoysis Moysidis0Angeliki Chorti1Angeliki Cheva2Ioanna Abba Deka3Georgios Tzikos4Christoforos Kosmidis5Ioannis Koutelidakis6Joulia K. Tsetis7Theodossis Papavramidis8Katerina Kotzampassi9Department of Surgery, Aristotle University of Thessaloniki, 54636 Thessaloniki, GreeceDepartment of Surgery, Aristotle University of Thessaloniki, 54636 Thessaloniki, GreeceDepartment of Pathology, Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceDepartment of Pathology, Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceDepartment of Surgery, Aristotle University of Thessaloniki, 54636 Thessaloniki, Greece3th Department of Surgery, AHEPA University Hospital, Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece2nd Department of Surgery, G. Gennimatas University Hospital, Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceUni-Pharma S.A., 14564 Athens, GreeceDepartment of Surgery, Aristotle University of Thessaloniki, 54636 Thessaloniki, GreeceDepartment of Surgery, Aristotle University of Thessaloniki, 54636 Thessaloniki, GreeceIntroduction: The utilization of probiotics in enhancing the active healing of skin wounds represents a burgeoning trend in contemporary medicine. Previous research has extensively explored wound healing mechanisms involving the strains of <i>Lactiplantibacillus plantarum</i>, <i>Lacticaseibacillus rhamnosus,</i> and <i>Bifidobacterium longum</i>. This study seeks to compare and interpret cellular findings derived from immunohistochemical and pathological applications. Methods: Three groups (the control, <i>Lactiplantibacillus plantarum</i> (RO1) group, and <i>Lacticaseibacillus rhamnosus</i> and <i>Bifidobacterium longum</i> (PRO2) group) underwent histological analysis, and microscopic cell counting were employed, offering insights into dynamic changes among neutrophils, lymphocytes, plasmacytes, mast cells, fibroblasts, and newly formed vessels across distinct treatment groups and temporal intervals. Results: The neutrophil count was found to be elevated in PRO2 on day 2, while the same group resulted in the highest decline on day 15. The number of fibroblasts peaked on day 4 for the PRO1 group, compared to the other two groups, which peaked on day 8. The lymphocyte count was the highest in the control group, while they peaked on day 4 in PRO2. The mast cells and plasmacytes were variable and sparse among all groups and time frames. Neovascularization was promoted by PRO1 and PRO2 groups on day 4 and remained high on day 8 for PRO2. Conclusions: Probiotic strains can be beneficial to the human population and in assisting skin wound healing, each strain working differently and more effectively in different healing phases. Thus, a combined formula containing different probiotics to modulate various healing phases is desirable.https://www.mdpi.com/1424-8247/17/11/1414probioticswound healing<i>L. plantarum</i><i>L. rhamnosum</i><i>B. longum</i> |
| spellingShingle | Moysis Moysidis Angeliki Chorti Angeliki Cheva Ioanna Abba Deka Georgios Tzikos Christoforos Kosmidis Ioannis Koutelidakis Joulia K. Tsetis Theodossis Papavramidis Katerina Kotzampassi <i>L. plantarum</i> UBLP-40 Versus the Combined Formula of <i>L. rhamnosus</i> UBLP-58 and <i>B. longum</i> UBBL-64 in Excisional Wound Healing: A Cellular Perspective Pharmaceuticals probiotics wound healing <i>L. plantarum</i> <i>L. rhamnosum</i> <i>B. longum</i> |
| title | <i>L. plantarum</i> UBLP-40 Versus the Combined Formula of <i>L. rhamnosus</i> UBLP-58 and <i>B. longum</i> UBBL-64 in Excisional Wound Healing: A Cellular Perspective |
| title_full | <i>L. plantarum</i> UBLP-40 Versus the Combined Formula of <i>L. rhamnosus</i> UBLP-58 and <i>B. longum</i> UBBL-64 in Excisional Wound Healing: A Cellular Perspective |
| title_fullStr | <i>L. plantarum</i> UBLP-40 Versus the Combined Formula of <i>L. rhamnosus</i> UBLP-58 and <i>B. longum</i> UBBL-64 in Excisional Wound Healing: A Cellular Perspective |
| title_full_unstemmed | <i>L. plantarum</i> UBLP-40 Versus the Combined Formula of <i>L. rhamnosus</i> UBLP-58 and <i>B. longum</i> UBBL-64 in Excisional Wound Healing: A Cellular Perspective |
| title_short | <i>L. plantarum</i> UBLP-40 Versus the Combined Formula of <i>L. rhamnosus</i> UBLP-58 and <i>B. longum</i> UBBL-64 in Excisional Wound Healing: A Cellular Perspective |
| title_sort | i l plantarum i ublp 40 versus the combined formula of i l rhamnosus i ublp 58 and i b longum i ubbl 64 in excisional wound healing a cellular perspective |
| topic | probiotics wound healing <i>L. plantarum</i> <i>L. rhamnosum</i> <i>B. longum</i> |
| url | https://www.mdpi.com/1424-8247/17/11/1414 |
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