Hydrolysis and Oxidation of Recalcitrant Substrates by Microbial Isolates from Hot Springs
Traditional energy sources pollute the environment. Microbial fuel cells are an alternative energy source that can reduce the environmental burden. Microbial fuel cells also remove recalcitrant wastes from wastewater. This research featured the enzymatic potential of microbial isolates obtained from...
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| Format: | Article |
| Language: | English |
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Kemerovo State University
2024-03-01
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| Series: | Техника и технология пищевых производств |
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| Online Access: | https://fptt.ru/en/issues/22328/22336/ |
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| author | Anastasiya I. Dmitrieva Elizaveta R. Faskhutdinova Timothy A. Larichev Natalia S. Velichkovich Veronika Yu. Boger Larisa M. Aksenova |
| author_facet | Anastasiya I. Dmitrieva Elizaveta R. Faskhutdinova Timothy A. Larichev Natalia S. Velichkovich Veronika Yu. Boger Larisa M. Aksenova |
| author_sort | Anastasiya I. Dmitrieva |
| collection | DOAJ |
| description | Traditional energy sources pollute the environment. Microbial fuel cells are an alternative energy source that can reduce the environmental burden. Microbial fuel cells also remove recalcitrant wastes from wastewater. This research featured the enzymatic potential of microbial isolates obtained from the Abakan Arzhan thermal spring.
The study involved isolates of the genera Geobacter, Thermomonas, and Rhodopseudomonas. The keratinolytic analysis was in line with State Standard R 55987-2014. The chitinolytic activity was determined by injecting a bacterial suspension on Petri dishes with a chitin-containing medium. The lipolytic analysis involved cultivating the isolates in Stern’s glycerol fuchsin broth. The xylan hydrolysis depended on the reducing sugars. The cellulase activity was measured according to the standard method recommended by the International Union of Pure and Applied Chemistry (IUPAC). The catalase potential was evaluated by the gasometric method on 1% gasoline media. The optimal parameters of consortium cultivation were determined by the voltage generated.
The Geobacter isolate had the maximal keratinolytic activity while the Thermomonas isolate demonstrated the maximal protein hydrolysis (80.1 ± 1.5%). Both Geobacter and Rhodopseudomonas showed good lytic activity against chitin with the lysis zone of ≥ 3 mm. The Geobacter isolate demonstrated as many as 350 units of xylanase activity and 365 units of cellulase activity; Thermomonas had 350 units of xylanase activity and 360 units of cellulase activity; Rhodopseudomonas showed 310 units of xylanase activity and 304 units of cellulase activity. The maximal catalase properties belonged to Geobacter (1.40 units) and Thermomonas (1.38 units). The maximal energy generation by bacterial consortia occurred at pH 8 and 45°C after 48 h of cultivation.
In this research, isolates of the genera Geobacter, Thermomonas, and Rhodopseudomonas from the Abakan Arzhan thermal spring were able to remove recalcitrant components, thus demonstrating good prospects for biological treatment of industrial wastewater. |
| format | Article |
| id | doaj-art-095dcad8ce254d1f803575e155c154e8 |
| institution | Kabale University |
| issn | 2074-9414 2313-1748 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Техника и технология пищевых производств |
| spelling | doaj-art-095dcad8ce254d1f803575e155c154e82025-01-02T23:19:26ZengKemerovo State UniversityТехника и технология пищевых производств2074-94142313-17482024-03-01541273610.21603/2074-9414-2024-1-2486Hydrolysis and Oxidation of Recalcitrant Substrates by Microbial Isolates from Hot SpringsAnastasiya I. Dmitrieva0https://orcid.org/0000-0002-8764-4049Elizaveta R. Faskhutdinova1https://orcid.org/0000-0001-9711-2145Timothy A. Larichev2https://orcid.org/0000-0002-3801-0023Natalia S. Velichkovich3https://orcid.org/0000-0002-9061-1256Veronika Yu. Boger4 Larisa M. Aksenova5Kemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaAll-Russian Scientific Research Institute of Confectionery Industry, Moscow, RussiaTraditional energy sources pollute the environment. Microbial fuel cells are an alternative energy source that can reduce the environmental burden. Microbial fuel cells also remove recalcitrant wastes from wastewater. This research featured the enzymatic potential of microbial isolates obtained from the Abakan Arzhan thermal spring. The study involved isolates of the genera Geobacter, Thermomonas, and Rhodopseudomonas. The keratinolytic analysis was in line with State Standard R 55987-2014. The chitinolytic activity was determined by injecting a bacterial suspension on Petri dishes with a chitin-containing medium. The lipolytic analysis involved cultivating the isolates in Stern’s glycerol fuchsin broth. The xylan hydrolysis depended on the reducing sugars. The cellulase activity was measured according to the standard method recommended by the International Union of Pure and Applied Chemistry (IUPAC). The catalase potential was evaluated by the gasometric method on 1% gasoline media. The optimal parameters of consortium cultivation were determined by the voltage generated. The Geobacter isolate had the maximal keratinolytic activity while the Thermomonas isolate demonstrated the maximal protein hydrolysis (80.1 ± 1.5%). Both Geobacter and Rhodopseudomonas showed good lytic activity against chitin with the lysis zone of ≥ 3 mm. The Geobacter isolate demonstrated as many as 350 units of xylanase activity and 365 units of cellulase activity; Thermomonas had 350 units of xylanase activity and 360 units of cellulase activity; Rhodopseudomonas showed 310 units of xylanase activity and 304 units of cellulase activity. The maximal catalase properties belonged to Geobacter (1.40 units) and Thermomonas (1.38 units). The maximal energy generation by bacterial consortia occurred at pH 8 and 45°C after 48 h of cultivation. In this research, isolates of the genera Geobacter, Thermomonas, and Rhodopseudomonas from the Abakan Arzhan thermal spring were able to remove recalcitrant components, thus demonstrating good prospects for biological treatment of industrial wastewater.https://fptt.ru/en/issues/22328/22336/environmentally friendly energyextremophilic microorganismsenzymatic activitymicrobial fuel cellhard-to-decompose substrates |
| spellingShingle | Anastasiya I. Dmitrieva Elizaveta R. Faskhutdinova Timothy A. Larichev Natalia S. Velichkovich Veronika Yu. Boger Larisa M. Aksenova Hydrolysis and Oxidation of Recalcitrant Substrates by Microbial Isolates from Hot Springs Техника и технология пищевых производств environmentally friendly energy extremophilic microorganisms enzymatic activity microbial fuel cell hard-to-decompose substrates |
| title | Hydrolysis and Oxidation of Recalcitrant Substrates by Microbial Isolates from Hot Springs |
| title_full | Hydrolysis and Oxidation of Recalcitrant Substrates by Microbial Isolates from Hot Springs |
| title_fullStr | Hydrolysis and Oxidation of Recalcitrant Substrates by Microbial Isolates from Hot Springs |
| title_full_unstemmed | Hydrolysis and Oxidation of Recalcitrant Substrates by Microbial Isolates from Hot Springs |
| title_short | Hydrolysis and Oxidation of Recalcitrant Substrates by Microbial Isolates from Hot Springs |
| title_sort | hydrolysis and oxidation of recalcitrant substrates by microbial isolates from hot springs |
| topic | environmentally friendly energy extremophilic microorganisms enzymatic activity microbial fuel cell hard-to-decompose substrates |
| url | https://fptt.ru/en/issues/22328/22336/ |
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