Histochemical Localization and Cytotoxic Potential of Alkaloids in <i>Phaedranassa lehmannii</i>
Plants of the subfamily Amaryllidoideae are a source of unique and bioactive alkaloids called Amaryllidaceae alkaloids. The study of their anticancer potential has intensified in recent years. This work aims to locate and characterize the profile of cytotoxic alkaloids biosynthesized and stored in d...
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2024-11-01
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| author | Lina M. Trujillo Chacón Hawer Leiva José M. Rojas Isabel C. Zapata Vahos Dagoberto Castro María Domínguez Edison Osorio |
| author_facet | Lina M. Trujillo Chacón Hawer Leiva José M. Rojas Isabel C. Zapata Vahos Dagoberto Castro María Domínguez Edison Osorio |
| author_sort | Lina M. Trujillo Chacón |
| collection | DOAJ |
| description | Plants of the subfamily Amaryllidoideae are a source of unique and bioactive alkaloids called Amaryllidaceae alkaloids. The study of their anticancer potential has intensified in recent years. This work aims to locate and characterize the profile of cytotoxic alkaloids biosynthesized and stored in different tissues of <i>Phaedranassa lehmannii</i> Regel using different histochemical methods and chromatographic analysis. The histochemical analysis in the bulbs revealed the presence of alkaloids at the basal edge of the scale-like leaves and bud apical zone. The GC-MS analysis indicated that the bulbs biosynthesize crinane- (9.80 µg/g DW), galanthamine- (8.04 µg/g DW), lycorine- (7.38 µg/g DW), and narciclasine-type (3.75 µg/g DW) alkaloids. The root biosynthesizes alkaloids that are mainly distributed mostly in lycorine- (225.29 µg/g DW) and galanthamine-type (72.35 µg/g DW) alkaloids. The total alkaloids biosynthesized by the root (324.93 µg/g DW) exceeded eleven times the abundance of the alkaloids identified in the bulbs (28.97 µg/g DW). In addition, the total alkaloid fractions exhibited a dose-dependent cytotoxic effect in the evaluated concentrations, with IC<sub>50</sub> values of 11.76 ± 0.99 µg/mL and 2.59 ± 0.56 µg/mL against human lung (A549) cancer cells and 8.00 ± 1.35 µg/mL and 18.74 ± 1.99 µg/mL against gastric (AGS) cancer cells. The present study provided evidence to locate and characterize the alkaloids of <i>P. lehmannii</i> grown under nursery conditions as a species producing potential antiproliferative alkaloids. |
| format | Article |
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| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-0941c00ec6b842b588cc1d6664baf3b22024-11-26T18:19:07ZengMDPI AGPlants2223-77472024-11-011322325110.3390/plants13223251Histochemical Localization and Cytotoxic Potential of Alkaloids in <i>Phaedranassa lehmannii</i>Lina M. Trujillo Chacón0Hawer Leiva1José M. Rojas2Isabel C. Zapata Vahos3Dagoberto Castro4María Domínguez5Edison Osorio6Grupo de Investigación en Sustancias Bioactivas GISB, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia, Calle 70 No. 52-21, Medellín 050010, ColombiaUnidad de Biotecnología Vegetal, Facultad de Ingeniería, Universidad Católica de Oriente, Rionegro 054040, ColombiaGrupo de Investigación Estudios Florísticos, Facultad de Ingeniería, Universidad Católica de Oriente, Apartado Aéreo 008, Rionegro 054040, ColombiaGrupo de Investigación Atención Primaria en Salud, Facultad de Ciencias de la Salud, Universidad Católica de Oriente, Rionegro 054040, ColombiaUnidad de Biotecnología Vegetal, Facultad de Ingeniería, Universidad Católica de Oriente, Rionegro 054040, ColombiaUnidad de Biotecnología Vegetal, Facultad de Ingeniería, Universidad Católica de Oriente, Rionegro 054040, ColombiaGrupo de Investigación en Sustancias Bioactivas GISB, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia, Calle 70 No. 52-21, Medellín 050010, ColombiaPlants of the subfamily Amaryllidoideae are a source of unique and bioactive alkaloids called Amaryllidaceae alkaloids. The study of their anticancer potential has intensified in recent years. This work aims to locate and characterize the profile of cytotoxic alkaloids biosynthesized and stored in different tissues of <i>Phaedranassa lehmannii</i> Regel using different histochemical methods and chromatographic analysis. The histochemical analysis in the bulbs revealed the presence of alkaloids at the basal edge of the scale-like leaves and bud apical zone. The GC-MS analysis indicated that the bulbs biosynthesize crinane- (9.80 µg/g DW), galanthamine- (8.04 µg/g DW), lycorine- (7.38 µg/g DW), and narciclasine-type (3.75 µg/g DW) alkaloids. The root biosynthesizes alkaloids that are mainly distributed mostly in lycorine- (225.29 µg/g DW) and galanthamine-type (72.35 µg/g DW) alkaloids. The total alkaloids biosynthesized by the root (324.93 µg/g DW) exceeded eleven times the abundance of the alkaloids identified in the bulbs (28.97 µg/g DW). In addition, the total alkaloid fractions exhibited a dose-dependent cytotoxic effect in the evaluated concentrations, with IC<sub>50</sub> values of 11.76 ± 0.99 µg/mL and 2.59 ± 0.56 µg/mL against human lung (A549) cancer cells and 8.00 ± 1.35 µg/mL and 18.74 ± 1.99 µg/mL against gastric (AGS) cancer cells. The present study provided evidence to locate and characterize the alkaloids of <i>P. lehmannii</i> grown under nursery conditions as a species producing potential antiproliferative alkaloids.https://www.mdpi.com/2223-7747/13/22/3251<i>Phaedranassa lehmannii</i>Amaryllidaceae alkaloidscytotoxicityhistochemical localization |
| spellingShingle | Lina M. Trujillo Chacón Hawer Leiva José M. Rojas Isabel C. Zapata Vahos Dagoberto Castro María Domínguez Edison Osorio Histochemical Localization and Cytotoxic Potential of Alkaloids in <i>Phaedranassa lehmannii</i> Plants <i>Phaedranassa lehmannii</i> Amaryllidaceae alkaloids cytotoxicity histochemical localization |
| title | Histochemical Localization and Cytotoxic Potential of Alkaloids in <i>Phaedranassa lehmannii</i> |
| title_full | Histochemical Localization and Cytotoxic Potential of Alkaloids in <i>Phaedranassa lehmannii</i> |
| title_fullStr | Histochemical Localization and Cytotoxic Potential of Alkaloids in <i>Phaedranassa lehmannii</i> |
| title_full_unstemmed | Histochemical Localization and Cytotoxic Potential of Alkaloids in <i>Phaedranassa lehmannii</i> |
| title_short | Histochemical Localization and Cytotoxic Potential of Alkaloids in <i>Phaedranassa lehmannii</i> |
| title_sort | histochemical localization and cytotoxic potential of alkaloids in i phaedranassa lehmannii i |
| topic | <i>Phaedranassa lehmannii</i> Amaryllidaceae alkaloids cytotoxicity histochemical localization |
| url | https://www.mdpi.com/2223-7747/13/22/3251 |
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