LungVis 1.0: an automatic AI-powered 3D imaging ecosystem unveils spatial profiling of nanoparticle delivery and acinar migration of lung macrophages
Abstract Targeted (nano-)drug delivery is essential for treating respiratory diseases, which are often confined to distinct lung regions. However, spatio-temporal profiling of drugs or nanoparticles (NPs) and their interactions with lung macrophages remains unresolved. Here, we present LungVis 1.0,...
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| Format: | Article |
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Nature Portfolio
2024-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-54267-1 |
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| author | Lin Yang Qiongliang Liu Pramod Kumar Arunima Sengupta Ali Farnoud Ruolin Shen Darya Trofimova Sebastian Ziegler Neda Davoudi Ali Doryab Ali Önder Yildirim Markus E. Diefenbacher Herbert B. Schiller Daniel Razansky Marie Piraud Gerald Burgstaller Wolfgang G. Kreyling Fabian Isensee Markus Rehberg Tobias Stoeger Otmar Schmid |
| author_facet | Lin Yang Qiongliang Liu Pramod Kumar Arunima Sengupta Ali Farnoud Ruolin Shen Darya Trofimova Sebastian Ziegler Neda Davoudi Ali Doryab Ali Önder Yildirim Markus E. Diefenbacher Herbert B. Schiller Daniel Razansky Marie Piraud Gerald Burgstaller Wolfgang G. Kreyling Fabian Isensee Markus Rehberg Tobias Stoeger Otmar Schmid |
| author_sort | Lin Yang |
| collection | DOAJ |
| description | Abstract Targeted (nano-)drug delivery is essential for treating respiratory diseases, which are often confined to distinct lung regions. However, spatio-temporal profiling of drugs or nanoparticles (NPs) and their interactions with lung macrophages remains unresolved. Here, we present LungVis 1.0, an AI-powered imaging ecosystem that integrates light sheet fluorescence microscopy with deep learning-based image analysis pipelines to map NP deposition and dosage holistically and quantitatively across bronchial and alveolar (acinar) regions in murine lungs for widely-used bulk-liquid and aerosol-based delivery methods. We demonstrate that bulk-liquid delivery results in patchy NP distribution with elevated bronchial doses, whereas aerosols achieve uniform deposition reaching distal alveoli. Furthermore, we reveal that lung tissue-resident macrophages (TRMs) are dynamic, actively patrolling and redistributing NPs within alveoli, contesting the conventional paradigm of TRMs as static entities. LungVis 1.0 provides an advanced framework for exploring pulmonary delivery dynamics and deepening insights into TRM-mediated lung immunity. |
| format | Article |
| id | doaj-art-09230b937a684643ae6ba3af933a693d |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-09230b937a684643ae6ba3af933a693d2024-12-01T12:34:19ZengNature PortfolioNature Communications2041-17232024-11-0115112210.1038/s41467-024-54267-1LungVis 1.0: an automatic AI-powered 3D imaging ecosystem unveils spatial profiling of nanoparticle delivery and acinar migration of lung macrophagesLin Yang0Qiongliang Liu1Pramod Kumar2Arunima Sengupta3Ali Farnoud4Ruolin Shen5Darya Trofimova6Sebastian Ziegler7Neda Davoudi8Ali Doryab9Ali Önder Yildirim10Markus E. Diefenbacher11Herbert B. Schiller12Daniel Razansky13Marie Piraud14Gerald Burgstaller15Wolfgang G. Kreyling16Fabian Isensee17Markus Rehberg18Tobias Stoeger19Otmar Schmid20Institute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL)Institute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL)Institute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL)Institute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL)Institute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL)Helmholtz AI, Helmholtz MunichHelmholtz Imaging, German Cancer Research Center (DKFZ)Helmholtz Imaging, German Cancer Research Center (DKFZ)Institute of Pharmacology and Toxicology and Institute for Biomedical Engineering, Faculty of Medicine, University of ZurichInstitute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL)Institute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL)Institute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL)Institute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL)Institute of Pharmacology and Toxicology and Institute for Biomedical Engineering, Faculty of Medicine, University of ZurichHelmholtz AI, Helmholtz MunichInstitute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL)Institute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL)Helmholtz Imaging, German Cancer Research Center (DKFZ)Institute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL)Institute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL)Institute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL)Abstract Targeted (nano-)drug delivery is essential for treating respiratory diseases, which are often confined to distinct lung regions. However, spatio-temporal profiling of drugs or nanoparticles (NPs) and their interactions with lung macrophages remains unresolved. Here, we present LungVis 1.0, an AI-powered imaging ecosystem that integrates light sheet fluorescence microscopy with deep learning-based image analysis pipelines to map NP deposition and dosage holistically and quantitatively across bronchial and alveolar (acinar) regions in murine lungs for widely-used bulk-liquid and aerosol-based delivery methods. We demonstrate that bulk-liquid delivery results in patchy NP distribution with elevated bronchial doses, whereas aerosols achieve uniform deposition reaching distal alveoli. Furthermore, we reveal that lung tissue-resident macrophages (TRMs) are dynamic, actively patrolling and redistributing NPs within alveoli, contesting the conventional paradigm of TRMs as static entities. LungVis 1.0 provides an advanced framework for exploring pulmonary delivery dynamics and deepening insights into TRM-mediated lung immunity.https://doi.org/10.1038/s41467-024-54267-1 |
| spellingShingle | Lin Yang Qiongliang Liu Pramod Kumar Arunima Sengupta Ali Farnoud Ruolin Shen Darya Trofimova Sebastian Ziegler Neda Davoudi Ali Doryab Ali Önder Yildirim Markus E. Diefenbacher Herbert B. Schiller Daniel Razansky Marie Piraud Gerald Burgstaller Wolfgang G. Kreyling Fabian Isensee Markus Rehberg Tobias Stoeger Otmar Schmid LungVis 1.0: an automatic AI-powered 3D imaging ecosystem unveils spatial profiling of nanoparticle delivery and acinar migration of lung macrophages Nature Communications |
| title | LungVis 1.0: an automatic AI-powered 3D imaging ecosystem unveils spatial profiling of nanoparticle delivery and acinar migration of lung macrophages |
| title_full | LungVis 1.0: an automatic AI-powered 3D imaging ecosystem unveils spatial profiling of nanoparticle delivery and acinar migration of lung macrophages |
| title_fullStr | LungVis 1.0: an automatic AI-powered 3D imaging ecosystem unveils spatial profiling of nanoparticle delivery and acinar migration of lung macrophages |
| title_full_unstemmed | LungVis 1.0: an automatic AI-powered 3D imaging ecosystem unveils spatial profiling of nanoparticle delivery and acinar migration of lung macrophages |
| title_short | LungVis 1.0: an automatic AI-powered 3D imaging ecosystem unveils spatial profiling of nanoparticle delivery and acinar migration of lung macrophages |
| title_sort | lungvis 1 0 an automatic ai powered 3d imaging ecosystem unveils spatial profiling of nanoparticle delivery and acinar migration of lung macrophages |
| url | https://doi.org/10.1038/s41467-024-54267-1 |
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