Clay Minerals Change the Toxic Effect of Cadmium on the Activities of Leucine Aminopeptidase
Soil leucine aminopeptidase (LAP) is a hydrolytic enzyme involved in the acquisition of nitrogen by microorganisms. In contaminated soils, LAP activity is affected not only by the type and concentration of heavy metals but also by the form of enzyme. Here, we investigated the degree and mechanism of...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2021-01-01
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| Series: | Adsorption Science & Technology |
| Online Access: | http://dx.doi.org/10.1155/2021/1024085 |
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| author | Shunyu Huang Jingji Li Jipeng Wang |
| author_facet | Shunyu Huang Jingji Li Jipeng Wang |
| author_sort | Shunyu Huang |
| collection | DOAJ |
| description | Soil leucine aminopeptidase (LAP) is a hydrolytic enzyme involved in the acquisition of nitrogen by microorganisms. In contaminated soils, LAP activity is affected not only by the type and concentration of heavy metals but also by the form of enzyme. Here, we investigated the degree and mechanism of cadmium (Cd) inhibition of soil LAP and purified LAP. We also examined the effect of montmorillonite and kaolinite on LAP and LAP contaminated with Cd. The results showed that Cd inhibition of LAP activity increased with increasing Cd concentration and that Cd exerted noncompetitive inhibition of LAP. The addition of clay minerals decreases LAP activity and the maximum reaction rate (Vmax), regardless of the presence of Cd. Montmorillonite decreases the affinity of LAP to the substrate (Km), while kaolinite increases the affinity of LAP to the substrate. The clay mineral-immobilized LAP showed an increase in resistance to Cd contamination compared with the free LAP. The results obtained in this study may aid in understanding the toxic effects of heavy metals on soil enzymes. |
| format | Article |
| id | doaj-art-090eabbe9af84950b2b98b43631deed9 |
| institution | Kabale University |
| issn | 0263-6174 2048-4038 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Adsorption Science & Technology |
| spelling | doaj-art-090eabbe9af84950b2b98b43631deed92025-01-02T22:54:09ZengSAGE PublishingAdsorption Science & Technology0263-61742048-40382021-01-01202110.1155/2021/10240851024085Clay Minerals Change the Toxic Effect of Cadmium on the Activities of Leucine AminopeptidaseShunyu Huang0Jingji Li1Jipeng Wang2State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, Chengdu University of Technology, 610059 Chengdu, ChinaState Key Laboratory of Geohazard Prevention and Geoenvironment Protection, Chengdu University of Technology, 610059 Chengdu, ChinaState Key Laboratory of Geohazard Prevention and Geoenvironment Protection, Chengdu University of Technology, 610059 Chengdu, ChinaSoil leucine aminopeptidase (LAP) is a hydrolytic enzyme involved in the acquisition of nitrogen by microorganisms. In contaminated soils, LAP activity is affected not only by the type and concentration of heavy metals but also by the form of enzyme. Here, we investigated the degree and mechanism of cadmium (Cd) inhibition of soil LAP and purified LAP. We also examined the effect of montmorillonite and kaolinite on LAP and LAP contaminated with Cd. The results showed that Cd inhibition of LAP activity increased with increasing Cd concentration and that Cd exerted noncompetitive inhibition of LAP. The addition of clay minerals decreases LAP activity and the maximum reaction rate (Vmax), regardless of the presence of Cd. Montmorillonite decreases the affinity of LAP to the substrate (Km), while kaolinite increases the affinity of LAP to the substrate. The clay mineral-immobilized LAP showed an increase in resistance to Cd contamination compared with the free LAP. The results obtained in this study may aid in understanding the toxic effects of heavy metals on soil enzymes.http://dx.doi.org/10.1155/2021/1024085 |
| spellingShingle | Shunyu Huang Jingji Li Jipeng Wang Clay Minerals Change the Toxic Effect of Cadmium on the Activities of Leucine Aminopeptidase Adsorption Science & Technology |
| title | Clay Minerals Change the Toxic Effect of Cadmium on the Activities of Leucine Aminopeptidase |
| title_full | Clay Minerals Change the Toxic Effect of Cadmium on the Activities of Leucine Aminopeptidase |
| title_fullStr | Clay Minerals Change the Toxic Effect of Cadmium on the Activities of Leucine Aminopeptidase |
| title_full_unstemmed | Clay Minerals Change the Toxic Effect of Cadmium on the Activities of Leucine Aminopeptidase |
| title_short | Clay Minerals Change the Toxic Effect of Cadmium on the Activities of Leucine Aminopeptidase |
| title_sort | clay minerals change the toxic effect of cadmium on the activities of leucine aminopeptidase |
| url | http://dx.doi.org/10.1155/2021/1024085 |
| work_keys_str_mv | AT shunyuhuang claymineralschangethetoxiceffectofcadmiumontheactivitiesofleucineaminopeptidase AT jingjili claymineralschangethetoxiceffectofcadmiumontheactivitiesofleucineaminopeptidase AT jipengwang claymineralschangethetoxiceffectofcadmiumontheactivitiesofleucineaminopeptidase |