Genes associated with cell modelling provides new insights into spermiation mechanism in Cyprinus carpio
Spermiation, an act of sperm release, depends on several molecular factors. Despite hormonal administration, spermiation failure is a primary concern in certain fishes. In this study, the molecular mechanisms of spermiation have been analyzed in Cyprinus carpio by comparative transcriptomics. Unigen...
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Elsevier
2024-01-01
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| Series: | Current Research in Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590262824000881 |
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| author | Ananya Khatei Janmejay Parhi Dibyajyoti Uttameswar Behera Partha Sarathi Tripathy Sagar Chandra Mandal Bijay Kumar Behera |
| author_facet | Ananya Khatei Janmejay Parhi Dibyajyoti Uttameswar Behera Partha Sarathi Tripathy Sagar Chandra Mandal Bijay Kumar Behera |
| author_sort | Ananya Khatei |
| collection | DOAJ |
| description | Spermiation, an act of sperm release, depends on several molecular factors. Despite hormonal administration, spermiation failure is a primary concern in certain fishes. In this study, the molecular mechanisms of spermiation have been analyzed in Cyprinus carpio by comparative transcriptomics. Unigenes for C. carpio control (CCC), which were injected with PBS (Phosphate-buffered saline), and C. carpio treated (CCT), which were injected with ovatide, were 107,616 and 133,435, respectively. A total of 93 genes were identified as involved in the spermiation process, including those related to gonadal steroidogenesis, cell growth, cell adhesion, and cytoplasmic matrix formation. The cd63, CENPS, rasa1a, and genes for gonad steroidogenesis, cell growth, cell adhesion, and cytoplasmic matrix formation were analyzed. Gene expression analysis revealed tubulobulbar complexes mediated disengagement of spermatozoa and JAK2 signaling regulated cyst breakage in teleost for the first time. Analysis was done from the changes at the molecular level to the final act of spermiation. Tissue histology analysis was conducted in accordance with the molecular study, which showed structural changes. Induced breeding in fish plays a key role in seed production in aquaculture sector. However, there are several constraints the sector is still facing due to lack of extensive knowledge regarding the mechanisms of spermiation and species-specific response to hormonal dosage. This study is relevant to understand the molecular mechanisms involved in spermiation and the stages which mark as critical point of sperm release after administrating the inducing agent. This study also lays the groundwork for further exploration of species-specific responses to hormonal treatments, aiding sustainable seed production in the fisheries sector. |
| format | Article |
| id | doaj-art-08fb725635a543f893e043b8f18a5635 |
| institution | Kabale University |
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| language | English |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
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| series | Current Research in Biotechnology |
| spelling | doaj-art-08fb725635a543f893e043b8f18a56352024-12-04T05:13:47ZengElsevierCurrent Research in Biotechnology2590-26282024-01-018100262Genes associated with cell modelling provides new insights into spermiation mechanism in Cyprinus carpioAnanya Khatei0Janmejay Parhi1Dibyajyoti Uttameswar Behera2Partha Sarathi Tripathy3Sagar Chandra Mandal4Bijay Kumar Behera5Faculty of Biosciences and Aquaculture, Nord University, Bodø 8026, NorwayCollege of Fisheries, Central Agricultural University (Imphal), Tripura 799210, India; Corresponding author.Shiksha ’O’ Anusandhan, Bhubaneswar, Odisha 751030, IndiaFaculty of Biosciences and Aquaculture, Nord University, Bodø 8026, Norway; College of Fisheries, Rani Lakshmibai Central Agricultural University, Jhansi, Uttar Pradesh 284003, India; Corresponding author.College of Fisheries, Central Agricultural University (Imphal), Tripura 799210, IndiaNational Fisheries Development Board, Hyderabad, Telangana 500052, India; Corresponding author.Spermiation, an act of sperm release, depends on several molecular factors. Despite hormonal administration, spermiation failure is a primary concern in certain fishes. In this study, the molecular mechanisms of spermiation have been analyzed in Cyprinus carpio by comparative transcriptomics. Unigenes for C. carpio control (CCC), which were injected with PBS (Phosphate-buffered saline), and C. carpio treated (CCT), which were injected with ovatide, were 107,616 and 133,435, respectively. A total of 93 genes were identified as involved in the spermiation process, including those related to gonadal steroidogenesis, cell growth, cell adhesion, and cytoplasmic matrix formation. The cd63, CENPS, rasa1a, and genes for gonad steroidogenesis, cell growth, cell adhesion, and cytoplasmic matrix formation were analyzed. Gene expression analysis revealed tubulobulbar complexes mediated disengagement of spermatozoa and JAK2 signaling regulated cyst breakage in teleost for the first time. Analysis was done from the changes at the molecular level to the final act of spermiation. Tissue histology analysis was conducted in accordance with the molecular study, which showed structural changes. Induced breeding in fish plays a key role in seed production in aquaculture sector. However, there are several constraints the sector is still facing due to lack of extensive knowledge regarding the mechanisms of spermiation and species-specific response to hormonal dosage. This study is relevant to understand the molecular mechanisms involved in spermiation and the stages which mark as critical point of sperm release after administrating the inducing agent. This study also lays the groundwork for further exploration of species-specific responses to hormonal treatments, aiding sustainable seed production in the fisheries sector.http://www.sciencedirect.com/science/article/pii/S2590262824000881Blood-Testis-BarrierEndoplasmic reticulumIntegrinSertoliSpermatidSteroids |
| spellingShingle | Ananya Khatei Janmejay Parhi Dibyajyoti Uttameswar Behera Partha Sarathi Tripathy Sagar Chandra Mandal Bijay Kumar Behera Genes associated with cell modelling provides new insights into spermiation mechanism in Cyprinus carpio Current Research in Biotechnology Blood-Testis-Barrier Endoplasmic reticulum Integrin Sertoli Spermatid Steroids |
| title | Genes associated with cell modelling provides new insights into spermiation mechanism in Cyprinus carpio |
| title_full | Genes associated with cell modelling provides new insights into spermiation mechanism in Cyprinus carpio |
| title_fullStr | Genes associated with cell modelling provides new insights into spermiation mechanism in Cyprinus carpio |
| title_full_unstemmed | Genes associated with cell modelling provides new insights into spermiation mechanism in Cyprinus carpio |
| title_short | Genes associated with cell modelling provides new insights into spermiation mechanism in Cyprinus carpio |
| title_sort | genes associated with cell modelling provides new insights into spermiation mechanism in cyprinus carpio |
| topic | Blood-Testis-Barrier Endoplasmic reticulum Integrin Sertoli Spermatid Steroids |
| url | http://www.sciencedirect.com/science/article/pii/S2590262824000881 |
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