Comparative analysis of improved m6A sequencing based on antibody optimization for low-input samples

Comparative analysis of improved m6A sequencing based on antibody optimization for low-input samples

Abstract The most effective method for mapping N6-methyladenosine (m6A) is m6A RNA immunoprecipitation sequencing (MeRIP-seq). The quality of MeRIP-seq relies on various factors, with the anti-m6A antibody being a crucial determinant. However, comprehensive research on anti-m6A antibody selection an...

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Bibliographic Details
Main Authors: Jiafeng Lu, Wenjuan Xia, Jincheng Li, Liya Zhang, Chunfeng Qian, Hong Li, Boxian Huang
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Scientific Reports
Subjects:
m6A antibody
MeRIP Seq
Human fetal brain
Human fetal liver
Mouse liver
Mouse brain
Online Access:https://doi.org/10.1038/s41598-025-85150-8
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