Metabolomic profiling identifies metabolites in the pheromone glands of Agriophara rhombata associated with the synthesis and release of female pheromone signals

The tea moth pest, Agriophara rhombata is an economically important and highly damaging pest that drastically affects tea plant leaves. The chemical composition of its pheromone glands metabolites before and during calling period has not been reported yet. Therefore, the present study aimed at the m...

Full description

Saved in:
Bibliographic Details
Main Authors: Hao Qu, Yaqin Long, Long Chen, Ziwen Luo, Hongyun Chen, Xuesong Wang, Lixue Long, Jun Tian, Tingting Jing, Linbo Chen
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Heliyon
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844024167990
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1846125369966460928
author Hao Qu
Yaqin Long
Long Chen
Ziwen Luo
Hongyun Chen
Xuesong Wang
Lixue Long
Jun Tian
Tingting Jing
Linbo Chen
author_facet Hao Qu
Yaqin Long
Long Chen
Ziwen Luo
Hongyun Chen
Xuesong Wang
Lixue Long
Jun Tian
Tingting Jing
Linbo Chen
author_sort Hao Qu
collection DOAJ
description The tea moth pest, Agriophara rhombata is an economically important and highly damaging pest that drastically affects tea plant leaves. The chemical composition of its pheromone glands metabolites before and during calling period has not been reported yet. Therefore, the present study aimed at the metabolomic profiling of female moths’ sex pheromones glands before and during calling period using gas chromatography time-of-flight mass spectrometry. A total of 114 significant differentially expressed metabolites were identified including 54 up- and 70 down-regulated metabolites in pheromone glands of the female moth. Two of the important previously recognized moth pheromones were identified including E,Z-5,7-dodecadien-1-ol acetate and Z-12-Octadecen-1-ol acetate, which were downregulated. The top ten up-regulated metabolites were “dodecanamide”, “tetradecanamide”, “2-propyn-1-amine, N,N-dimethyl”, “cyclohexane, (1-methylethyl)”, “tetradecane, 2-methyl”, “1-cyclopentyleicosane”, “cyclohexane, octyl”, “1-decan-3-one”, “cyclopentane, decyl” and “cyclopentadecane”. In conclusion, while most of the identified compounds have not previously been identified as primary pheromones in moths, their differential expression in A. rhombata's pheromone glands during the calling period strongly suggests their supporting roles in the synthesis, stabilization, or release of the active pheromone components.
format Article
id doaj-art-087b611404604dba8e26814100c6b1aa
institution Kabale University
issn 2405-8440
language English
publishDate 2024-12-01
publisher Elsevier
record_format Article
series Heliyon
spelling doaj-art-087b611404604dba8e26814100c6b1aa2024-12-13T10:59:45ZengElsevierHeliyon2405-84402024-12-011023e40768Metabolomic profiling identifies metabolites in the pheromone glands of Agriophara rhombata associated with the synthesis and release of female pheromone signalsHao Qu0Yaqin Long1Long Chen2Ziwen Luo3Hongyun Chen4Xuesong Wang5Lixue Long6Jun Tian7Tingting Jing8Linbo Chen9Tea Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650000, China; Yunnan Provincial Key Laboratory of Tea Science, Menghai, 666201, ChinaTea Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650000, China; Yunnan Provincial Key Laboratory of Tea Science, Menghai, 666201, ChinaTea Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650000, China; Yunnan Provincial Key Laboratory of Tea Science, Menghai, 666201, ChinaTea Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650000, China; Yunnan Provincial Key Laboratory of Tea Science, Menghai, 666201, ChinaTea Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650000, China; Yunnan Provincial Key Laboratory of Tea Science, Menghai, 666201, ChinaTea Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650000, China; Yunnan Provincial Key Laboratory of Tea Science, Menghai, 666201, ChinaTea Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650000, China; Yunnan Provincial Key Laboratory of Tea Science, Menghai, 666201, ChinaKunming Colourful Yunnan King-shine Tea Industry Co., Ltd, Kunming, 650500, ChinaState Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects, Anhui Agricultural University, 230036, Hefei, Anhui, China; Corresponding author.Tea Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650000, China; Yunnan Provincial Key Laboratory of Tea Science, Menghai, 666201, China; Corresponding author. Tea Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650000, China.The tea moth pest, Agriophara rhombata is an economically important and highly damaging pest that drastically affects tea plant leaves. The chemical composition of its pheromone glands metabolites before and during calling period has not been reported yet. Therefore, the present study aimed at the metabolomic profiling of female moths’ sex pheromones glands before and during calling period using gas chromatography time-of-flight mass spectrometry. A total of 114 significant differentially expressed metabolites were identified including 54 up- and 70 down-regulated metabolites in pheromone glands of the female moth. Two of the important previously recognized moth pheromones were identified including E,Z-5,7-dodecadien-1-ol acetate and Z-12-Octadecen-1-ol acetate, which were downregulated. The top ten up-regulated metabolites were “dodecanamide”, “tetradecanamide”, “2-propyn-1-amine, N,N-dimethyl”, “cyclohexane, (1-methylethyl)”, “tetradecane, 2-methyl”, “1-cyclopentyleicosane”, “cyclohexane, octyl”, “1-decan-3-one”, “cyclopentane, decyl” and “cyclopentadecane”. In conclusion, while most of the identified compounds have not previously been identified as primary pheromones in moths, their differential expression in A. rhombata's pheromone glands during the calling period strongly suggests their supporting roles in the synthesis, stabilization, or release of the active pheromone components.http://www.sciencedirect.com/science/article/pii/S2405844024167990Tea mothPheromone glandsMetabolomic profilingSex pheromones
spellingShingle Hao Qu
Yaqin Long
Long Chen
Ziwen Luo
Hongyun Chen
Xuesong Wang
Lixue Long
Jun Tian
Tingting Jing
Linbo Chen
Metabolomic profiling identifies metabolites in the pheromone glands of Agriophara rhombata associated with the synthesis and release of female pheromone signals
Heliyon
Tea moth
Pheromone glands
Metabolomic profiling
Sex pheromones
title Metabolomic profiling identifies metabolites in the pheromone glands of Agriophara rhombata associated with the synthesis and release of female pheromone signals
title_full Metabolomic profiling identifies metabolites in the pheromone glands of Agriophara rhombata associated with the synthesis and release of female pheromone signals
title_fullStr Metabolomic profiling identifies metabolites in the pheromone glands of Agriophara rhombata associated with the synthesis and release of female pheromone signals
title_full_unstemmed Metabolomic profiling identifies metabolites in the pheromone glands of Agriophara rhombata associated with the synthesis and release of female pheromone signals
title_short Metabolomic profiling identifies metabolites in the pheromone glands of Agriophara rhombata associated with the synthesis and release of female pheromone signals
title_sort metabolomic profiling identifies metabolites in the pheromone glands of agriophara rhombata associated with the synthesis and release of female pheromone signals
topic Tea moth
Pheromone glands
Metabolomic profiling
Sex pheromones
url http://www.sciencedirect.com/science/article/pii/S2405844024167990
work_keys_str_mv AT haoqu metabolomicprofilingidentifiesmetabolitesinthepheromoneglandsofagriophararhombataassociatedwiththesynthesisandreleaseoffemalepheromonesignals
AT yaqinlong metabolomicprofilingidentifiesmetabolitesinthepheromoneglandsofagriophararhombataassociatedwiththesynthesisandreleaseoffemalepheromonesignals
AT longchen metabolomicprofilingidentifiesmetabolitesinthepheromoneglandsofagriophararhombataassociatedwiththesynthesisandreleaseoffemalepheromonesignals
AT ziwenluo metabolomicprofilingidentifiesmetabolitesinthepheromoneglandsofagriophararhombataassociatedwiththesynthesisandreleaseoffemalepheromonesignals
AT hongyunchen metabolomicprofilingidentifiesmetabolitesinthepheromoneglandsofagriophararhombataassociatedwiththesynthesisandreleaseoffemalepheromonesignals
AT xuesongwang metabolomicprofilingidentifiesmetabolitesinthepheromoneglandsofagriophararhombataassociatedwiththesynthesisandreleaseoffemalepheromonesignals
AT lixuelong metabolomicprofilingidentifiesmetabolitesinthepheromoneglandsofagriophararhombataassociatedwiththesynthesisandreleaseoffemalepheromonesignals
AT juntian metabolomicprofilingidentifiesmetabolitesinthepheromoneglandsofagriophararhombataassociatedwiththesynthesisandreleaseoffemalepheromonesignals
AT tingtingjing metabolomicprofilingidentifiesmetabolitesinthepheromoneglandsofagriophararhombataassociatedwiththesynthesisandreleaseoffemalepheromonesignals
AT linbochen metabolomicprofilingidentifiesmetabolitesinthepheromoneglandsofagriophararhombataassociatedwiththesynthesisandreleaseoffemalepheromonesignals