Effect of Every-other-day Fasting on Level of Serum TNF-α in Rats after Acute Spinal Cord Injury
Objective:To observe the effect of every-other-day fasting (EODF) on serum tumor necrosis factor-α (TNF-α) level in rats after acute spinal cord injury.Methods:A total of 48 female adult Wistar rats were randomly divided into EODF group (group A), normal diet control group (group B) and sham-operate...
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Editorial Office of Rehabilitation Medicine
2015-12-01
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| Series: | 康复学报 |
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| Online Access: | http://kfxb.publish.founderss.cn/thesisDetails#10.13261/j.issn.2096-0328.2015.04.004 |
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| author | Jiancheng LIU Wenchun WANG Shaojie LUO Yanbo LI Yunhui ZHAO Rizhao PANG Chao DONG Shanshan ZHANG Anren ZHANG |
| author_facet | Jiancheng LIU Wenchun WANG Shaojie LUO Yanbo LI Yunhui ZHAO Rizhao PANG Chao DONG Shanshan ZHANG Anren ZHANG |
| author_sort | Jiancheng LIU |
| collection | DOAJ |
| description | Objective:To observe the effect of every-other-day fasting (EODF) on serum tumor necrosis factor-α (TNF-α) level in rats after acute spinal cord injury.Methods:A total of 48 female adult Wistar rats were randomly divided into EODF group (group A), normal diet control group (group B) and sham-operated group (group C). Thoracic spinal cord injury models were established in rats of group A and group B by modified Allen contusion method, and laminectomy was performed in rats of group C. Then group A received EODF, group B and group C were given a normal diet immediately after operation.BBB scores were calculated in each group at 6 hours, 1st day, 3rd day and 7th day after operation to observe the motor function changes, then the rats were killed and their serum was taken. The level of TNF-α in serum was detected by ELISA.Results:Severe hindlimb motor dysfunction of rats was found in group A and group B after operation, and there were significant differences in BBB scores between the two groups at 3rd day and 7th day after operation (<italic>P</italic><0.05). The level of serum TNF-α was increased in group A and group B after operation, and there were significant differences in the index between the two groups at 1st day, 3rd day and 7th day after operation (<italic>P</italic><0.05).Conclusion:EODF can improve the hind limb motor function of rats after acute spinal cord injury. The mechanism may be related with suppressing expression of inflammatory cytokines TNF-α, inhibiting inflammatory response and reducing secondary injury. |
| format | Article |
| id | doaj-art-085f99c3c0fe45f6bd806c13d7e5da22 |
| institution | Kabale University |
| issn | 2096-0328 |
| language | English |
| publishDate | 2015-12-01 |
| publisher | Editorial Office of Rehabilitation Medicine |
| record_format | Article |
| series | 康复学报 |
| spelling | doaj-art-085f99c3c0fe45f6bd806c13d7e5da222025-01-14T10:06:35ZengEditorial Office of Rehabilitation Medicine康复学报2096-03282015-12-0125141723094854Effect of Every-other-day Fasting on Level of Serum TNF-α in Rats after Acute Spinal Cord InjuryJiancheng LIUWenchun WANGShaojie LUOYanbo LIYunhui ZHAORizhao PANGChao DONGShanshan ZHANGAnren ZHANGObjective:To observe the effect of every-other-day fasting (EODF) on serum tumor necrosis factor-α (TNF-α) level in rats after acute spinal cord injury.Methods:A total of 48 female adult Wistar rats were randomly divided into EODF group (group A), normal diet control group (group B) and sham-operated group (group C). Thoracic spinal cord injury models were established in rats of group A and group B by modified Allen contusion method, and laminectomy was performed in rats of group C. Then group A received EODF, group B and group C were given a normal diet immediately after operation.BBB scores were calculated in each group at 6 hours, 1st day, 3rd day and 7th day after operation to observe the motor function changes, then the rats were killed and their serum was taken. The level of TNF-α in serum was detected by ELISA.Results:Severe hindlimb motor dysfunction of rats was found in group A and group B after operation, and there were significant differences in BBB scores between the two groups at 3rd day and 7th day after operation (<italic>P</italic><0.05). The level of serum TNF-α was increased in group A and group B after operation, and there were significant differences in the index between the two groups at 1st day, 3rd day and 7th day after operation (<italic>P</italic><0.05).Conclusion:EODF can improve the hind limb motor function of rats after acute spinal cord injury. The mechanism may be related with suppressing expression of inflammatory cytokines TNF-α, inhibiting inflammatory response and reducing secondary injury.http://kfxb.publish.founderss.cn/thesisDetails#10.13261/j.issn.2096-0328.2015.04.004spinal cord injuryevery-other-day fastingTNF-α |
| spellingShingle | Jiancheng LIU Wenchun WANG Shaojie LUO Yanbo LI Yunhui ZHAO Rizhao PANG Chao DONG Shanshan ZHANG Anren ZHANG Effect of Every-other-day Fasting on Level of Serum TNF-α in Rats after Acute Spinal Cord Injury 康复学报 spinal cord injury every-other-day fasting TNF-α |
| title | Effect of Every-other-day Fasting on Level of Serum TNF-α in Rats after Acute Spinal Cord Injury |
| title_full | Effect of Every-other-day Fasting on Level of Serum TNF-α in Rats after Acute Spinal Cord Injury |
| title_fullStr | Effect of Every-other-day Fasting on Level of Serum TNF-α in Rats after Acute Spinal Cord Injury |
| title_full_unstemmed | Effect of Every-other-day Fasting on Level of Serum TNF-α in Rats after Acute Spinal Cord Injury |
| title_short | Effect of Every-other-day Fasting on Level of Serum TNF-α in Rats after Acute Spinal Cord Injury |
| title_sort | effect of every other day fasting on level of serum tnf α in rats after acute spinal cord injury |
| topic | spinal cord injury every-other-day fasting TNF-α |
| url | http://kfxb.publish.founderss.cn/thesisDetails#10.13261/j.issn.2096-0328.2015.04.004 |
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