Cellular response to short-time mechanical stimuli: mediating Ca2+ influx via PIEZO1
Physical activity shows a positive correlation with overall health, and vigorous intermittent lifestyle physical activity (VILPA) similarly offers advantages in reducing the risk of all-cause mortality. Might the short-time mechanical stimuli be discernible to cells, eliciting commensurate physiolog...
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| Format: | Article |
| Language: | English |
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Forum Multimedia Publishing LLC
2024-07-01
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| Series: | European Cells & Materials |
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| Online Access: | https://www.ecmjournal.org/papers/vol048/vol048a01.php |
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| author | Fuan Wang Hongkun Chen Zhongyuan He Jianfeng Li Zhengya Zhu Tao Tang Junhong Li Jiaxiang Zhou Qiuxiao Tan Zhen Li Martin J. Stoddart Xizhe Liu Manman Gao Zhiyu Zhou Shaoyu Liu |
| author_facet | Fuan Wang Hongkun Chen Zhongyuan He Jianfeng Li Zhengya Zhu Tao Tang Junhong Li Jiaxiang Zhou Qiuxiao Tan Zhen Li Martin J. Stoddart Xizhe Liu Manman Gao Zhiyu Zhou Shaoyu Liu |
| author_sort | Fuan Wang |
| collection | DOAJ |
| description | Physical activity shows a positive correlation with overall health, and vigorous intermittent lifestyle physical activity (VILPA) similarly offers advantages in reducing the risk of all-cause mortality. Might the short-time mechanical stimuli be discernible to cells, eliciting commensurate physiological responses? The study's objective was to investigate the cellular response to short-time mechanical stimuli. Human umbilical cord-derived mesenchymal stem cells (hUCMSCs), isolated and thoroughly characterized, were subjected to various stimuli, including activation and mechanical stretching, with Ca2+ influx assessed through alterations in fluorescence intensity. Further validation of these findings was confirmed through short hairpin RNA (shRNA) and inhibitors. In addition, a comprehensive examination of PIEZO1 alterations was conducted through quantitative real-time polymerase chain reaction (qRT-PCR) and western blot (WB) techniques. The results shown different frequencies of stretching stimulation and durations induced varying degrees of Ca2+ influx. The most substantial increase occurred within 2–3 minutes in the group subjected to 0.5 Hz stretching for 2 minutes (p < 0.05). Stretching at 0.5 Hz resulted in significant elevation in PIEZO1 mRNA expression at 15 minutes and 1 hour. Additionally, stretching cause a gradual rise in PIEZO1 protein levels, with a notable peak observed at 2 hours. In conclusion, cells primarily sense short-time mechanical stimuli through PIEZO1, predominantly mediated by regulated Ca2+ influx. This underscores PIEZO1's crucial role in cellular responsiveness to transient mechanical cues, advancing our understanding of mechanosensory mechanisms in cellular physiology. |
| format | Article |
| id | doaj-art-0854d11bca4a4a3f950faddb5e7257b5 |
| institution | Kabale University |
| issn | 1473-2262 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Forum Multimedia Publishing LLC |
| record_format | Article |
| series | European Cells & Materials |
| spelling | doaj-art-0854d11bca4a4a3f950faddb5e7257b52024-12-17T09:33:20ZengForum Multimedia Publishing LLCEuropean Cells & Materials1473-22622024-07-014811610.22203/eCM.v048a01Cellular response to short-time mechanical stimuli: mediating Ca2+ influx via PIEZO1Fuan WangHongkun ChenZhongyuan HeJianfeng LiZhengya ZhuTao TangJunhong LiJiaxiang ZhouQiuxiao TanZhen LiMartin J. StoddartXizhe LiuManman GaoZhiyu ZhouShaoyu LiuPhysical activity shows a positive correlation with overall health, and vigorous intermittent lifestyle physical activity (VILPA) similarly offers advantages in reducing the risk of all-cause mortality. Might the short-time mechanical stimuli be discernible to cells, eliciting commensurate physiological responses? The study's objective was to investigate the cellular response to short-time mechanical stimuli. Human umbilical cord-derived mesenchymal stem cells (hUCMSCs), isolated and thoroughly characterized, were subjected to various stimuli, including activation and mechanical stretching, with Ca2+ influx assessed through alterations in fluorescence intensity. Further validation of these findings was confirmed through short hairpin RNA (shRNA) and inhibitors. In addition, a comprehensive examination of PIEZO1 alterations was conducted through quantitative real-time polymerase chain reaction (qRT-PCR) and western blot (WB) techniques. The results shown different frequencies of stretching stimulation and durations induced varying degrees of Ca2+ influx. The most substantial increase occurred within 2–3 minutes in the group subjected to 0.5 Hz stretching for 2 minutes (p < 0.05). Stretching at 0.5 Hz resulted in significant elevation in PIEZO1 mRNA expression at 15 minutes and 1 hour. Additionally, stretching cause a gradual rise in PIEZO1 protein levels, with a notable peak observed at 2 hours. In conclusion, cells primarily sense short-time mechanical stimuli through PIEZO1, predominantly mediated by regulated Ca2+ influx. This underscores PIEZO1's crucial role in cellular responsiveness to transient mechanical cues, advancing our understanding of mechanosensory mechanisms in cellular physiology.https://www.ecmjournal.org/papers/vol048/vol048a01.phpvigorous intermittent lifestyle physical activityshort-time mechanical stimulipiezo1ca2+ influxyoda1 |
| spellingShingle | Fuan Wang Hongkun Chen Zhongyuan He Jianfeng Li Zhengya Zhu Tao Tang Junhong Li Jiaxiang Zhou Qiuxiao Tan Zhen Li Martin J. Stoddart Xizhe Liu Manman Gao Zhiyu Zhou Shaoyu Liu Cellular response to short-time mechanical stimuli: mediating Ca2+ influx via PIEZO1 European Cells & Materials vigorous intermittent lifestyle physical activity short-time mechanical stimuli piezo1 ca2+ influx yoda1 |
| title | Cellular response to short-time mechanical stimuli: mediating Ca2+ influx via PIEZO1 |
| title_full | Cellular response to short-time mechanical stimuli: mediating Ca2+ influx via PIEZO1 |
| title_fullStr | Cellular response to short-time mechanical stimuli: mediating Ca2+ influx via PIEZO1 |
| title_full_unstemmed | Cellular response to short-time mechanical stimuli: mediating Ca2+ influx via PIEZO1 |
| title_short | Cellular response to short-time mechanical stimuli: mediating Ca2+ influx via PIEZO1 |
| title_sort | cellular response to short time mechanical stimuli mediating ca2 influx via piezo1 |
| topic | vigorous intermittent lifestyle physical activity short-time mechanical stimuli piezo1 ca2+ influx yoda1 |
| url | https://www.ecmjournal.org/papers/vol048/vol048a01.php |
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