Intracellular dry mass density increases under growth-induced pressure [version 2; peer review: 1 approved, 2 approved with reservations]
Cells that proliferate in confined environments develop mechanical compressive stress, referred to as growth-induced pressure, which inhibits growth and division across various organisms. Recent studies have shown that in these confined spaces, the diffusivity of intracellular nanoparticles decrease...
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2024-12-01
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| Series: | Open Research Europe |
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| Online Access: | https://open-research-europe.ec.europa.eu/articles/4-231/v2 |
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| _version_ | 1841562435744432128 |
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| author | Baptiste Alric Hyojun Kim Julien Roul Nolan Chan Morgan Delarue |
| author_facet | Baptiste Alric Hyojun Kim Julien Roul Nolan Chan Morgan Delarue |
| author_sort | Baptiste Alric |
| collection | DOAJ |
| description | Cells that proliferate in confined environments develop mechanical compressive stress, referred to as growth-induced pressure, which inhibits growth and division across various organisms. Recent studies have shown that in these confined spaces, the diffusivity of intracellular nanoparticles decreases. However, the physical mechanisms behind this reduction remain unclear. In this study, we use quantitative phase imaging to measure the refractive index and dry mass density of Saccharomyces cerevisiae cells proliferating under confinement in a microfluidic bioreactor. Our results indicate that the observed decrease in diffusivity could be attributed to the intracellular accumulation of macromolecules. Furthermore, the linear scaling between cell content and growth-induced pressure suggests that the concentrations of macromolecules and osmolytes are maintained proportionally under such pressure in S. cerevisiae. |
| format | Article |
| id | doaj-art-08397f4091bd442d91bb13ba67ba94c1 |
| institution | Kabale University |
| issn | 2732-5121 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | Open Research Europe |
| spelling | doaj-art-08397f4091bd442d91bb13ba67ba94c12025-01-03T01:00:00ZengF1000 Research LtdOpen Research Europe2732-51212024-12-01420720Intracellular dry mass density increases under growth-induced pressure [version 2; peer review: 1 approved, 2 approved with reservations]Baptiste Alric0Hyojun Kim1Julien Roul2Nolan Chan3Morgan Delarue4Institute of Industrial Science, Tokyo University, Tokyo, JapanLAAS-CNRS, University of Toulouse, Toulouse, FranceLAAS-CNRS, University of Toulouse, Toulouse, FrancePhasics, Bâtiment Mercury I, Espace Technologique, Route de l’Orme des Merisiers, St. Aubin, FranceLAAS-CNRS, University of Toulouse, Toulouse, FranceCells that proliferate in confined environments develop mechanical compressive stress, referred to as growth-induced pressure, which inhibits growth and division across various organisms. Recent studies have shown that in these confined spaces, the diffusivity of intracellular nanoparticles decreases. However, the physical mechanisms behind this reduction remain unclear. In this study, we use quantitative phase imaging to measure the refractive index and dry mass density of Saccharomyces cerevisiae cells proliferating under confinement in a microfluidic bioreactor. Our results indicate that the observed decrease in diffusivity could be attributed to the intracellular accumulation of macromolecules. Furthermore, the linear scaling between cell content and growth-induced pressure suggests that the concentrations of macromolecules and osmolytes are maintained proportionally under such pressure in S. cerevisiae.https://open-research-europe.ec.europa.eu/articles/4-231/v2macromolecular crowding microfluidic growth-induced pressure quantitative phase imaging refractive index dry mass densityeng |
| spellingShingle | Baptiste Alric Hyojun Kim Julien Roul Nolan Chan Morgan Delarue Intracellular dry mass density increases under growth-induced pressure [version 2; peer review: 1 approved, 2 approved with reservations] Open Research Europe macromolecular crowding microfluidic growth-induced pressure quantitative phase imaging refractive index dry mass density eng |
| title | Intracellular dry mass density increases under growth-induced pressure [version 2; peer review: 1 approved, 2 approved with reservations] |
| title_full | Intracellular dry mass density increases under growth-induced pressure [version 2; peer review: 1 approved, 2 approved with reservations] |
| title_fullStr | Intracellular dry mass density increases under growth-induced pressure [version 2; peer review: 1 approved, 2 approved with reservations] |
| title_full_unstemmed | Intracellular dry mass density increases under growth-induced pressure [version 2; peer review: 1 approved, 2 approved with reservations] |
| title_short | Intracellular dry mass density increases under growth-induced pressure [version 2; peer review: 1 approved, 2 approved with reservations] |
| title_sort | intracellular dry mass density increases under growth induced pressure version 2 peer review 1 approved 2 approved with reservations |
| topic | macromolecular crowding microfluidic growth-induced pressure quantitative phase imaging refractive index dry mass density eng |
| url | https://open-research-europe.ec.europa.eu/articles/4-231/v2 |
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