Profile of miRNAs induced during sheep fat tail development and roles of four key miRNAs in proliferation and differentiation of sheep preadipocytes
BackgroundThe fat tail of sheep is an adaptive trait that facilitates their adaptation to harsh natural environments. MicroRNAs (miRNAs) have been demonstrated to play crucial roles in the regulation of tail fat deposition.MethodsIn this study, miRNA-Seq was employed to investigate the expression pr...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-12-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1491160/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846142168188583936 |
|---|---|
| author | Wei Zhang Shiyin Wang Liwei Yang Li Gao Chengcheng Ning Mengsi Xu Shuangyi Deng Shangquan Gan |
| author_facet | Wei Zhang Shiyin Wang Liwei Yang Li Gao Chengcheng Ning Mengsi Xu Shuangyi Deng Shangquan Gan |
| author_sort | Wei Zhang |
| collection | DOAJ |
| description | BackgroundThe fat tail of sheep is an adaptive trait that facilitates their adaptation to harsh natural environments. MicroRNAs (miRNAs) have been demonstrated to play crucial roles in the regulation of tail fat deposition.MethodsIn this study, miRNA-Seq was employed to investigate the expression profiles of miRNAs during different developmental stages of sheep fat tails and elucidate the functions of differentially expressed miRNAs (DE miRNAs).ResultsA total of 350 DE miRNAs were identified, among which 191, 60, 26, and 21 were significantly upregulated in tail fat tissues of fetal, lamb, hogget Altay sheep, and adult Xinjiang fine wool (XFW) sheep but downregulated in other stages. Furthermore, we predicted a set of candidate target genes (4,476) for the top 20 DE miRNAs. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that they involve in several adipogenesis-related pathways. Subsequent investigations indicated that four DE miRNAs, miR-433-3p, miR-485-3p, miR-409-3p, and miR-495-3p, could suppress the expression of peroxisome proliferator-activated receptor gamma (PPARγ) and phosphoinositide-3-kinase regulatory subunit 3 (PIK3R3) and regulate the preadipocyte development in sheep. Meanwhile, the lipid metabolism-related genes, fatty acid-binding protein (FABP3), perilipin 1 (PLIN1), adiponectin C1Q and collagen domain containing (ADIPOQ), and lipoprotein lipase (LPL), were significantly downregulated (p < 0.01).ConclusionThe expression patterns of miRNAs exhibited significant fluctuations during different development periods of the fat tail, and some of them may participate in the regulation of tail fat deposition by modulating the proliferation and differentiation of preadipocytes. |
| format | Article |
| id | doaj-art-081134c2f302408f86b47313e1b4eb11 |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-081134c2f302408f86b47313e1b4eb112024-12-03T15:58:48ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-12-011110.3389/fvets.2024.14911601491160Profile of miRNAs induced during sheep fat tail development and roles of four key miRNAs in proliferation and differentiation of sheep preadipocytesWei Zhang0Shiyin Wang1Liwei Yang2Li Gao3Chengcheng Ning4Mengsi Xu5Shuangyi Deng6Shangquan Gan7Key Laboratory of Livestock and Poultry Healthy Breeding Technology in Northwest China, Xinjiang Agricultural Vocational and Technical University, Changji, ChinaKey Laboratory of Livestock and Poultry Healthy Breeding Technology in Northwest China, Xinjiang Agricultural Vocational and Technical University, Changji, ChinaKey Laboratory of Livestock and Poultry Healthy Breeding Technology in Northwest China, Xinjiang Agricultural Vocational and Technical University, Changji, ChinaKey Laboratory of Livestock and Poultry Healthy Breeding Technology in Northwest China, Xinjiang Agricultural Vocational and Technical University, Changji, ChinaKey Laboratory of Livestock and Poultry Healthy Breeding Technology in Northwest China, Xinjiang Agricultural Vocational and Technical University, Changji, ChinaState Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, ChinaKey Laboratory of Livestock and Poultry Healthy Breeding Technology in Northwest China, Xinjiang Agricultural Vocational and Technical University, Changji, ChinaCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, ChinaBackgroundThe fat tail of sheep is an adaptive trait that facilitates their adaptation to harsh natural environments. MicroRNAs (miRNAs) have been demonstrated to play crucial roles in the regulation of tail fat deposition.MethodsIn this study, miRNA-Seq was employed to investigate the expression profiles of miRNAs during different developmental stages of sheep fat tails and elucidate the functions of differentially expressed miRNAs (DE miRNAs).ResultsA total of 350 DE miRNAs were identified, among which 191, 60, 26, and 21 were significantly upregulated in tail fat tissues of fetal, lamb, hogget Altay sheep, and adult Xinjiang fine wool (XFW) sheep but downregulated in other stages. Furthermore, we predicted a set of candidate target genes (4,476) for the top 20 DE miRNAs. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that they involve in several adipogenesis-related pathways. Subsequent investigations indicated that four DE miRNAs, miR-433-3p, miR-485-3p, miR-409-3p, and miR-495-3p, could suppress the expression of peroxisome proliferator-activated receptor gamma (PPARγ) and phosphoinositide-3-kinase regulatory subunit 3 (PIK3R3) and regulate the preadipocyte development in sheep. Meanwhile, the lipid metabolism-related genes, fatty acid-binding protein (FABP3), perilipin 1 (PLIN1), adiponectin C1Q and collagen domain containing (ADIPOQ), and lipoprotein lipase (LPL), were significantly downregulated (p < 0.01).ConclusionThe expression patterns of miRNAs exhibited significant fluctuations during different development periods of the fat tail, and some of them may participate in the regulation of tail fat deposition by modulating the proliferation and differentiation of preadipocytes.https://www.frontiersin.org/articles/10.3389/fvets.2024.1491160/fullmiRNAsheepfat tissuetailpreadipocyteadipogenesis |
| spellingShingle | Wei Zhang Shiyin Wang Liwei Yang Li Gao Chengcheng Ning Mengsi Xu Shuangyi Deng Shangquan Gan Profile of miRNAs induced during sheep fat tail development and roles of four key miRNAs in proliferation and differentiation of sheep preadipocytes Frontiers in Veterinary Science miRNA sheep fat tissue tail preadipocyte adipogenesis |
| title | Profile of miRNAs induced during sheep fat tail development and roles of four key miRNAs in proliferation and differentiation of sheep preadipocytes |
| title_full | Profile of miRNAs induced during sheep fat tail development and roles of four key miRNAs in proliferation and differentiation of sheep preadipocytes |
| title_fullStr | Profile of miRNAs induced during sheep fat tail development and roles of four key miRNAs in proliferation and differentiation of sheep preadipocytes |
| title_full_unstemmed | Profile of miRNAs induced during sheep fat tail development and roles of four key miRNAs in proliferation and differentiation of sheep preadipocytes |
| title_short | Profile of miRNAs induced during sheep fat tail development and roles of four key miRNAs in proliferation and differentiation of sheep preadipocytes |
| title_sort | profile of mirnas induced during sheep fat tail development and roles of four key mirnas in proliferation and differentiation of sheep preadipocytes |
| topic | miRNA sheep fat tissue tail preadipocyte adipogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1491160/full |
| work_keys_str_mv | AT weizhang profileofmirnasinducedduringsheepfattaildevelopmentandrolesoffourkeymirnasinproliferationanddifferentiationofsheeppreadipocytes AT shiyinwang profileofmirnasinducedduringsheepfattaildevelopmentandrolesoffourkeymirnasinproliferationanddifferentiationofsheeppreadipocytes AT liweiyang profileofmirnasinducedduringsheepfattaildevelopmentandrolesoffourkeymirnasinproliferationanddifferentiationofsheeppreadipocytes AT ligao profileofmirnasinducedduringsheepfattaildevelopmentandrolesoffourkeymirnasinproliferationanddifferentiationofsheeppreadipocytes AT chengchengning profileofmirnasinducedduringsheepfattaildevelopmentandrolesoffourkeymirnasinproliferationanddifferentiationofsheeppreadipocytes AT mengsixu profileofmirnasinducedduringsheepfattaildevelopmentandrolesoffourkeymirnasinproliferationanddifferentiationofsheeppreadipocytes AT shuangyideng profileofmirnasinducedduringsheepfattaildevelopmentandrolesoffourkeymirnasinproliferationanddifferentiationofsheeppreadipocytes AT shangquangan profileofmirnasinducedduringsheepfattaildevelopmentandrolesoffourkeymirnasinproliferationanddifferentiationofsheeppreadipocytes |