In Situ Phytoremediation of Mine Tailings with High Concentrations of Cadmium and Lead Using <i>Dodonaea viscosa</i> (Sapindaceae)
The waste generated during metal mining activities contains mixtures of heavy metals (HM) that are not biodegradable and can accumulate in the surrounding biota, increasing risk to human and environmental health. Plant species with the capacity to grow and develop on mine tailings can be used as a m...
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2024-12-01
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| author | Luis Fernando Acosta-Núñez Patricia Mussali-Galante María Luisa Castrejón-Godínez Alexis Rodríguez-Solís Joel Daniel Castañeda-Espinoza Efraín Tovar-Sánchez |
| author_facet | Luis Fernando Acosta-Núñez Patricia Mussali-Galante María Luisa Castrejón-Godínez Alexis Rodríguez-Solís Joel Daniel Castañeda-Espinoza Efraín Tovar-Sánchez |
| author_sort | Luis Fernando Acosta-Núñez |
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| description | The waste generated during metal mining activities contains mixtures of heavy metals (HM) that are not biodegradable and can accumulate in the surrounding biota, increasing risk to human and environmental health. Plant species with the capacity to grow and develop on mine tailings can be used as a model system in phytoremediation studies. <i>Dodonaea viscosa</i> (L.) Jacq. is a shrub with wide geographical distribution and the ability to establish itself in mine tailings. The Sierra de Huautla Biosphere Reserve in Mexico contains a metallurgic district where mining activities have generated 780 million kg of waste with large concentrations of toxic heavy metals, mainly cadmium and lead. The present study evaluated the phytoremediation potential of <i>D. viscosa</i> in in situ conditions on soils contaminated with HMs (exposed) and reference sites (non-exposed) for one year. Also, the effects of cadmium (Cd) and lead (Pb) exposure in <i>D. viscosa</i> were analyzed via DNA damage (comet assay) morphological and physiological characters in exposed <i>vs</i> non-exposed individuals. The concentration of Cd and Pb was measured through atomic absorption spectrophotometry in the roots and leaves of plants. In total, 120 <i>D. viscosa</i> individuals were established, 60 growing in exposed and 60 in non-exposed soils. Exposed individuals of <i>D. viscosa</i> hyperaccumulated Cd and Pb in roots and leaves. At the end of the experiment, eight out of twelve characters under evaluation decreased significantly in HM-exposed plants in relation to individuals growing in non-exposed soils, except for stomatal index, stomatal coverage, and fresh leaf biomass. The micro-morphological and physiological traits of <i>D. viscosa</i> were not influenced by Cd and Pb bioaccumulation. In contrast, the bioaccumulation of Cd and Pb significantly influenced the macro-morphological characters and genetic damage; this last biomarker was 3.2 times higher in plants growing in exposed sites. The bioconcentration factor (BCF) of Cd and Pb in root and leaf tissue increased significantly over time. The mean BCF in root and leaf tissue was higher for Pb (877.58 and 798.77) than for Cd (50.86 and 23.02). After 12 months of exposure, <i>D. viscosa</i> individuals growing on mine tailing substrate showed that the total HM phytoextraction capacity was 7.56 kg∙ha<sup>−1</sup> for Pb and 0.307 kg∙ha<sup>−1</sup> for Cd. <i>D. viscosa</i> shows potential for phytoremediation of soils contaminated with Cd and Pb, given its capacity for establishing and developing naturally in contaminated soils with HM. Along with its bioaccumulation, biomass production, abundance, and high levels of bioconcentration factors, but without affecting plant development and not registering associated herbivores, it may incorporate HM into the trophic chain. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-080632ae16b244f6a666bcc643a8198b2025-01-10T13:19:39ZengMDPI AGPlants2223-77472024-12-011416910.3390/plants14010069In Situ Phytoremediation of Mine Tailings with High Concentrations of Cadmium and Lead Using <i>Dodonaea viscosa</i> (Sapindaceae)Luis Fernando Acosta-Núñez0Patricia Mussali-Galante1María Luisa Castrejón-Godínez2Alexis Rodríguez-Solís3Joel Daniel Castañeda-Espinoza4Efraín Tovar-Sánchez5Maestría en Manejo de Recursos Naturales, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, MexicoLaboratorio de Investigaciones Ambientales, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, MexicoFacultad de Ciencias Biológicas, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, MexicoLaboratorio de Investigaciones Ambientales, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, MexicoDepartamento de Interacción Planta-Insecto, Laboratorio de Entomología, Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Carretera Yautepec-Jojutla Km 6, Calle Ceprobi No. 8, Col. San Isidro, Yautepec 62731, Morelos, MexicoCentro de Investigación en Biodiversidad y Conservación, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, MexicoThe waste generated during metal mining activities contains mixtures of heavy metals (HM) that are not biodegradable and can accumulate in the surrounding biota, increasing risk to human and environmental health. Plant species with the capacity to grow and develop on mine tailings can be used as a model system in phytoremediation studies. <i>Dodonaea viscosa</i> (L.) Jacq. is a shrub with wide geographical distribution and the ability to establish itself in mine tailings. The Sierra de Huautla Biosphere Reserve in Mexico contains a metallurgic district where mining activities have generated 780 million kg of waste with large concentrations of toxic heavy metals, mainly cadmium and lead. The present study evaluated the phytoremediation potential of <i>D. viscosa</i> in in situ conditions on soils contaminated with HMs (exposed) and reference sites (non-exposed) for one year. Also, the effects of cadmium (Cd) and lead (Pb) exposure in <i>D. viscosa</i> were analyzed via DNA damage (comet assay) morphological and physiological characters in exposed <i>vs</i> non-exposed individuals. The concentration of Cd and Pb was measured through atomic absorption spectrophotometry in the roots and leaves of plants. In total, 120 <i>D. viscosa</i> individuals were established, 60 growing in exposed and 60 in non-exposed soils. Exposed individuals of <i>D. viscosa</i> hyperaccumulated Cd and Pb in roots and leaves. At the end of the experiment, eight out of twelve characters under evaluation decreased significantly in HM-exposed plants in relation to individuals growing in non-exposed soils, except for stomatal index, stomatal coverage, and fresh leaf biomass. The micro-morphological and physiological traits of <i>D. viscosa</i> were not influenced by Cd and Pb bioaccumulation. In contrast, the bioaccumulation of Cd and Pb significantly influenced the macro-morphological characters and genetic damage; this last biomarker was 3.2 times higher in plants growing in exposed sites. The bioconcentration factor (BCF) of Cd and Pb in root and leaf tissue increased significantly over time. The mean BCF in root and leaf tissue was higher for Pb (877.58 and 798.77) than for Cd (50.86 and 23.02). After 12 months of exposure, <i>D. viscosa</i> individuals growing on mine tailing substrate showed that the total HM phytoextraction capacity was 7.56 kg∙ha<sup>−1</sup> for Pb and 0.307 kg∙ha<sup>−1</sup> for Cd. <i>D. viscosa</i> shows potential for phytoremediation of soils contaminated with Cd and Pb, given its capacity for establishing and developing naturally in contaminated soils with HM. Along with its bioaccumulation, biomass production, abundance, and high levels of bioconcentration factors, but without affecting plant development and not registering associated herbivores, it may incorporate HM into the trophic chain.https://www.mdpi.com/2223-7747/14/1/69biocharmine tailingphytostabilizationgenetic damagehyperaccumulator |
| spellingShingle | Luis Fernando Acosta-Núñez Patricia Mussali-Galante María Luisa Castrejón-Godínez Alexis Rodríguez-Solís Joel Daniel Castañeda-Espinoza Efraín Tovar-Sánchez In Situ Phytoremediation of Mine Tailings with High Concentrations of Cadmium and Lead Using <i>Dodonaea viscosa</i> (Sapindaceae) Plants biochar mine tailing phytostabilization genetic damage hyperaccumulator |
| title | In Situ Phytoremediation of Mine Tailings with High Concentrations of Cadmium and Lead Using <i>Dodonaea viscosa</i> (Sapindaceae) |
| title_full | In Situ Phytoremediation of Mine Tailings with High Concentrations of Cadmium and Lead Using <i>Dodonaea viscosa</i> (Sapindaceae) |
| title_fullStr | In Situ Phytoremediation of Mine Tailings with High Concentrations of Cadmium and Lead Using <i>Dodonaea viscosa</i> (Sapindaceae) |
| title_full_unstemmed | In Situ Phytoremediation of Mine Tailings with High Concentrations of Cadmium and Lead Using <i>Dodonaea viscosa</i> (Sapindaceae) |
| title_short | In Situ Phytoremediation of Mine Tailings with High Concentrations of Cadmium and Lead Using <i>Dodonaea viscosa</i> (Sapindaceae) |
| title_sort | in situ phytoremediation of mine tailings with high concentrations of cadmium and lead using i dodonaea viscosa i sapindaceae |
| topic | biochar mine tailing phytostabilization genetic damage hyperaccumulator |
| url | https://www.mdpi.com/2223-7747/14/1/69 |
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