Effects of Inhaled Dexamethasone/Ciprofloxacin on Acute Subglottic Stenosis in a Rabbit Model
ABSTRACT Objectives Subglottic stenosis (SGS) is a challenging complication post‐airway interventions. Effective preventive strategies lack sufficient evidence. This study investigated the preventive effect of inhalation therapy with dexamethasone/ciprofloxacin on acute SGS in a rabbit model. Method...
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| Format: | Article |
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Wiley
2025-04-01
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| Series: | Laryngoscope Investigative Otolaryngology |
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| Online Access: | https://doi.org/10.1002/lio2.70119 |
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| author | I‐Chun Kuo Catherine K. Hart Isabelle M. Gengler Alessandro deAlarcón |
| author_facet | I‐Chun Kuo Catherine K. Hart Isabelle M. Gengler Alessandro deAlarcón |
| author_sort | I‐Chun Kuo |
| collection | DOAJ |
| description | ABSTRACT Objectives Subglottic stenosis (SGS) is a challenging complication post‐airway interventions. Effective preventive strategies lack sufficient evidence. This study investigated the preventive effect of inhalation therapy with dexamethasone/ciprofloxacin on acute SGS in a rabbit model. Methods Twenty New Zealand White rabbits underwent subglottic injury via endoscopy and received inhalation therapy twice daily starting on the injury day. Group 1 (n = 4) received saline for 5 days and was sacrificed on day 5; Group 2 (n = 4) received dexamethasone/ciprofloxacin for 5 days and was sacrificed on day 5; Group 3 (n = 4) received the same therapy for 5 days and survived for one week, and was sacrificed on day 15; Group 4 (n = 4) received the therapy for 10 days and was sacrificed on day 15; Group 5 (n = 4) received the therapy for 10 days and survived for one week, and was sacrificed on day 22. Rabbits underwent repeat endoscopy and were euthanized at the designated time point. Histological measurements were analyzed statistically. Results Histological analysis revealed median cricoid lumen measurements of 20.01 ± 1.42 mm2 for group 1, 17.94 ± 3.05 mm2 for group 2, 14.84 ± 2.55 mm2 for group 3, 17.18 ± 5.31 mm2 for group 4, and 11.87 ± 5.68 mm2 for group 5. No significant differences were found between treatment and control groups (p = 0.486) or between 5‐day and 10‐day treatments (p = 0.686). Multivariate statistical analysis indicated that cessation of inhalation therapy (p < 0.05) and prolonged survival (p < 0.05) were associated with shorter cricoid lumen measurements. Conclusion Short‐term dexamethasone/ciprofloxacin inhalation does not prevent acute SGS. No improvements in cricoid lumen diameter were found. Extended survival correlated with shorter cricoid lumen, suggesting SGS progression is time dependent. Level of Evidence NA. |
| format | Article |
| id | doaj-art-078a8b0753904b32b91ae4fe64f4a70e |
| institution | Kabale University |
| issn | 2378-8038 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Laryngoscope Investigative Otolaryngology |
| spelling | doaj-art-078a8b0753904b32b91ae4fe64f4a70e2025-08-20T03:53:43ZengWileyLaryngoscope Investigative Otolaryngology2378-80382025-04-01102n/an/a10.1002/lio2.70119Effects of Inhaled Dexamethasone/Ciprofloxacin on Acute Subglottic Stenosis in a Rabbit ModelI‐Chun Kuo0Catherine K. Hart1Isabelle M. Gengler2Alessandro deAlarcón3Department of Otolaryngology‐Head & Neck Surgery Chang Gung Memorial Hospital Taoyuan TaiwanDivision of Pediatric Otolaryngology–Head and Neck Surgery Cincinnati Children's Hospital Medical Center Cincinnati Ohio USADepartment of Otolaryngology–Head and Neck Surgery, College of Medicine University of Cincinnati Cincinnati Ohio USADivision of Pediatric Otolaryngology–Head and Neck Surgery Cincinnati Children's Hospital Medical Center Cincinnati Ohio USAABSTRACT Objectives Subglottic stenosis (SGS) is a challenging complication post‐airway interventions. Effective preventive strategies lack sufficient evidence. This study investigated the preventive effect of inhalation therapy with dexamethasone/ciprofloxacin on acute SGS in a rabbit model. Methods Twenty New Zealand White rabbits underwent subglottic injury via endoscopy and received inhalation therapy twice daily starting on the injury day. Group 1 (n = 4) received saline for 5 days and was sacrificed on day 5; Group 2 (n = 4) received dexamethasone/ciprofloxacin for 5 days and was sacrificed on day 5; Group 3 (n = 4) received the same therapy for 5 days and survived for one week, and was sacrificed on day 15; Group 4 (n = 4) received the therapy for 10 days and was sacrificed on day 15; Group 5 (n = 4) received the therapy for 10 days and survived for one week, and was sacrificed on day 22. Rabbits underwent repeat endoscopy and were euthanized at the designated time point. Histological measurements were analyzed statistically. Results Histological analysis revealed median cricoid lumen measurements of 20.01 ± 1.42 mm2 for group 1, 17.94 ± 3.05 mm2 for group 2, 14.84 ± 2.55 mm2 for group 3, 17.18 ± 5.31 mm2 for group 4, and 11.87 ± 5.68 mm2 for group 5. No significant differences were found between treatment and control groups (p = 0.486) or between 5‐day and 10‐day treatments (p = 0.686). Multivariate statistical analysis indicated that cessation of inhalation therapy (p < 0.05) and prolonged survival (p < 0.05) were associated with shorter cricoid lumen measurements. Conclusion Short‐term dexamethasone/ciprofloxacin inhalation does not prevent acute SGS. No improvements in cricoid lumen diameter were found. Extended survival correlated with shorter cricoid lumen, suggesting SGS progression is time dependent. Level of Evidence NA.https://doi.org/10.1002/lio2.70119animal modeldexamethasone/ciprofloxacininhalationsubglottic stenosis |
| spellingShingle | I‐Chun Kuo Catherine K. Hart Isabelle M. Gengler Alessandro deAlarcón Effects of Inhaled Dexamethasone/Ciprofloxacin on Acute Subglottic Stenosis in a Rabbit Model Laryngoscope Investigative Otolaryngology animal model dexamethasone/ciprofloxacin inhalation subglottic stenosis |
| title | Effects of Inhaled Dexamethasone/Ciprofloxacin on Acute Subglottic Stenosis in a Rabbit Model |
| title_full | Effects of Inhaled Dexamethasone/Ciprofloxacin on Acute Subglottic Stenosis in a Rabbit Model |
| title_fullStr | Effects of Inhaled Dexamethasone/Ciprofloxacin on Acute Subglottic Stenosis in a Rabbit Model |
| title_full_unstemmed | Effects of Inhaled Dexamethasone/Ciprofloxacin on Acute Subglottic Stenosis in a Rabbit Model |
| title_short | Effects of Inhaled Dexamethasone/Ciprofloxacin on Acute Subglottic Stenosis in a Rabbit Model |
| title_sort | effects of inhaled dexamethasone ciprofloxacin on acute subglottic stenosis in a rabbit model |
| topic | animal model dexamethasone/ciprofloxacin inhalation subglottic stenosis |
| url | https://doi.org/10.1002/lio2.70119 |
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